Isolation and study of active ATP-dependent phosphofructokinase from apple fruits Pyrus domestica Borkh

Isolation and study of active ATP-dependent phosphofructokinase from apple fruits Pyrus domestica Borkh

The activity of ATP-dependent phosphofructokinase (PFK) from subepidermal tissue of apple fruits was studied. The enzyme extracted under optimal conditions was stable for 14 h at room temperature. The enzyme was partially purified by ammonium sulfate fractionation and dialysis. PFK from apple fruits...

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Bibliographic Details
Published in:Applied biochemistry and microbiology Vol. 42; no. 5; pp. 468 - 471
Main Authors: Gyulakhmedov, S. G., Omarov, Y. A., Mamedov, Z. M., Kuliev, A. A.
Format: Journal Article
Language:English
Published: Dordrecht Springer Nature B.V 01-09-2006
Subjects:
Adenosine diphosphate
Ammonium
Apples
ATP
Cations
Cultivars
Dialysis
Fractionation
Fruits
Magnesium
Malus
Pyrus
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Summary:The activity of ATP-dependent phosphofructokinase (PFK) from subepidermal tissue of apple fruits was studied. The enzyme extracted under optimal conditions was stable for 14 h at room temperature. The enzyme was partially purified by ammonium sulfate fractionation and dialysis. PFK from apple fruits was found to be ATP-, UTP-, and CTP-specific. It was inhibited by PEP, Gly-2-P, Gly-1,3-DP, and ADP. The effect of the listed inhibitors was diminished by the presence of phosphate. The activity of PFK was stimulated by magnesium cations. The activity of the enzyme in fruits of an Antonovka cultivar was higher than in the Simirenko rennet cultivar by a factor of 1.3.[PUBLICATION ABSTRACT]
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ISSN:0003-6838
1608-3024
DOI:10.1134/S0003683806050048