Evaluation of Different Molecular Markers for Genotyping Non-Saccharomyces Wine Yeast Species

Evaluation of Different Molecular Markers for Genotyping Non-Saccharomyces Wine Yeast Species

Wine quality is determined by the particular yeast strains prevailing at various stages of fermentation. Therefore, the ability to make an easy, fast, and unambiguous discrimination of yeasts at the strain level is of great importance. Here, the tandem repeat-tRNA (TRtRNA) method with the 5GAC or IS...

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Bibliographic Details
Published in:Microbiology research Vol. 13; no. 3; pp. 643 - 654
Main Authors: Nisiotou, Aspasia, Gyftogianni, Emanouela, Banilas, Georgios
Format: Journal Article
Language:English
Published: Perugia MDPI AG 01-09-2022
Subjects:
Ethanol
Fermentation
Fingerprinting
Genotyping
Methods
molecular fingerprinting
non-Saccharomyces yeasts
Oligonucleotides
Polymerase chain reaction
Random amplified polymorphic DNA
RAPD
Saccharomyces
Species
Transfer RNA
tRNA
TRtRNA
Wine
wine fermentation
Wineries & vineyards
Wines
Yeast
Yeasts
United Kingdom > UK
England
Crete
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Summary:Wine quality is determined by the particular yeast strains prevailing at various stages of fermentation. Therefore, the ability to make an easy, fast, and unambiguous discrimination of yeasts at the strain level is of great importance. Here, the tandem repeat-tRNA (TRtRNA) method with the 5GAC or ISSR-MB primer sets and random amplified polymorphic DNA (RAPD) analysis with (GTG)3, R5, and RF2 oligonucleotides were tested on various non-Saccharomyces wine yeast species. The TRtRNA-PCR employing ISSR-MB showed the highest capacity in discriminating Lachancea thermotolerans and Metschnikowia pulcherrima isolates. RAPD with RF2 was the most efficient method in resolving Starmerella bacillaris isolates, although it produced few polymorphic bands. RAPD with R5 showed the highest capacity to discriminate among the Issatchenkia orientalis, Hanseniaspora guilliermondii, and Pichia anomala isolates. RAPD with either R5 or RF2 exhibited the highest ability to discriminate among the Torulaspora delbrueckii isolates. RAPD with (GTG)3 was the most discriminating method for the H. uvarum isolates. Here we concluded that both TRtRNA-PCR and RAPD-PCR offer rapid means for typing non-Saccharomyces species. However, each method performs better for a given species when paired with a particular primer set. The present results can be useful in wine research for the fast fingerprinting of non-Saccharomyces yeasts.
ISSN:2036-7481
2036-7481
DOI:10.3390/microbiolres13030046