New differentiation antigens associated with the growth and maturation of B lymphocytes

Monoclonal antibodies (mAb) NDA3 and NDA4 were generated by hyperimmunizing mice with an alloreactive human T cell clone and fusing the splenocytes with the NS1 myeloma. Immunofluorescence studies indicated that these mAb react with activated, but not with resting T and B lymphocytes or with other t...

Full description

Saved in:

Bibliographic Details
Published in:	The Journal of immunology (1950) Vol. 140; no. 2; pp. 395 - 403
Main Authors:	Suciu-Foca, N, Cai, JD, Gutierrea, C, Reed, E, Morrison, S, Pernis, B, King, DW
Format:	Journal Article
Language:	English
Published:	Bethesda, MD Am Assoc Immnol 15-01-1988 American Association of Immunologists
Subjects:	Animals Antibodies, Monoclonal - immunology Antigens, Differentiation - analysis Antigens, Differentiation - immunology B-Lymphocytes - cytology B-Lymphocytes - immunology Biological and medical sciences Cell Division - drug effects Cell Line, Transformed Fundamental and applied biological sciences. Psychology Fundamental immunology Herpesvirus 4, Human Humans Immunobiology Immunoglobulins - biosynthesis Interferons - pharmacology Lymphocyte Activation - drug effects Lymphoid cells: ontogeny, maturation, markers, receptors, circulation and recirculation Mice Mice, Inbred BALB C Precipitin Tests Receptors, Interleukin-4 Receptors, Mitogen - analysis Receptors, Mitogen - physiology Tetradecanoylphorbol Acetate - pharmacology
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Monoclonal antibodies (mAb) NDA3 and NDA4 were generated by hyperimmunizing mice with an alloreactive human T cell clone and fusing the splenocytes with the NS1 myeloma. Immunofluorescence studies indicated that these mAb react with activated, but not with resting T and B lymphocytes or with other types of cells. Immunoprecipitation studies with 125-I-labeled extracts from T and B cell lines demonstrated that the m.w. of the antigen recognized by mAb NDA3 is 36,000 and that of NDA4 is 46,000. Studies on the effect of mAb NDA3 and NDA4 on Epstein Barr Virus-transformed lymphoblastoid B cell lines (LBCL) demonstrated that these antibodies stimulate B cell proliferation and Ig synthesis. The level of expression of NDA3 and NDA4 on the membrane of LBCL is significantly augmented when cells are grown in the presence of recombinant human interferon-beta and gamma and is decreased in the presence of 12-O-tetradecanoyl-phorbol-13-acetate. These observations, in conjunction with the stimulatory effect of mAb NDA3 and NDA4 on the growth and differentiation of LBCL, suggest that these new differentiation antigens, NDA3 and NDA4, may serve as growth factor receptors.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23
ISSN:	0022-1767 1550-6606
DOI:	10.4049/jimmunol.140.2.395