Search Results - "Guseinov, T O"
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1
Infrared fluorescent markers for microarray DNA analysis
Published in Molecular biology (New York) (01-09-2015)“…In order to expand the informational capabilities of molecular genetic research, new indotricarbocyanine dyes that fluoresce in the near infrared (IR) spectral…”
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2
Comparative Study of Novel Fluorescent Cyanine Nucleotides: Hybridization Analysis of Labeled PCR Products Using a Biochip
Published in Journal of fluorescence (01-11-2017)“…This study investigated the synthesis and substrate properties of Cy5-labeled dUTP derivatives with different substituents, linkers between the dye unit and…”
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3
A Simultaneous Use of Cy5-Modified Derivatives of Deoxyuridine and Deoxycytidine in PCR
Published in Russian journal of bioorganic chemistry (01-07-2020)“…Particular features of the simultaneous incorporation into the growing DNA chain of modified Cy5-deoxyuridines (Cy5-dU) and deoxycytidines (Cy5-dC) and Taq DNA…”
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4
Preparation of Modified Combinatorial DNA Libraries via Emulsion PCR with Subsequent Strand Separation
Published in Molecular biology (New York) (01-11-2018)“…— A modification of the enzymatic method for the preparation of combinatorial random DNA libraries, which combines amplification in isolated microvolumes with…”
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5
Enzymatic Preparation of Modified DNA: Study of the Kinetics by Real-Time PCR
Published in Molecular biology (New York) (01-05-2019)“…The effects of modified deoxyuridine triphosphates (mod-dUTPs) with different substituents at the C5 position of the pyrimidine cycle on the kinetics of PCR…”
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6
Amplification Efficiency and Substrate Properties of Fluorescently Labeled Deoxyuridine Triphosphates in PCR in the Presence of DNA Polymerases without 3'-5' Exonuclease Activity
Published in Russian journal of bioorganic chemistry (01-07-2019)“…The kinetics of PCR amplification has been studied using fluorescently labeled zwitterionic deoxyuridine triphosphates that contain different fluorophores…”
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7
Fluorescent Labeling of Oligonucleotide Probes for Double Indicator Microarray Hybridization Analysis
Published in Russian journal of bioorganic chemistry (01-05-2019)“…Herein, we present a synthesis of a molecular construct based on the polysulfonated indocarbocyanine dye and the lysine. The chemical structure of the…”
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8
Substrate Properties of New Fluorescently Labeled Deoxycytidine Triphosphates in Enzymatic Synthesis of DNA with Polymerases of Families A and B
Published in Molecular biology (New York) (01-05-2018)“…The efficiency of the incorporation of fluorescently labeled derivatives of 2'-deoxycytidine in DNA synthesized de novo has been studied using PCR with Taq and…”
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9
Novel 5-Alkylcarboxamide-2'-Deoxyuridine-5'-Triphosphates for Enzymatic Synthesis of Highly Modified DNA
Published in Russian journal of bioorganic chemistry (01-05-2019)“…The synthesis of deoxyuridine triphosphate derivatives with alkyl groups of various chain length attached to the pyrimidine base by the trans -alkene linker…”
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10
Substrate Properties of New Fluorescently Labeled Deoxycytidine Triphosphates in Enzymatic Synthesis of DNA with Polymerases of Families A and B
Published in Molekuliarnaia biologiia (01-05-2018)“…The efficiency of the incorporation of fluorescently labeled derivatives of 2'-deoxycytidine in DNA synthesized de novo has been studied using PCR with Taq and…”
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11
Structural and functional analysis of biopolymers and their complexes: Enzymatic synthesis of high-modified DNA
Published in Molecular biology (New York) (01-05-2017)“…Here, we describe the synthesis and purification of six deoxyuridine triphosphate derivatives that contain protein-like functional groups and alkene linkers of…”
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12
New Synthetic Route to CY5-Labeled 2'-Deoxycytidine- 5'-Triphosphates Using Sonogashira Reaction
Published in Russian journal of bioorganic chemistry (01-03-2018)“…Herein, we present a new synthetic route to fluorescently-labeled nucleoside triphosphates via Sonogashira cross-coupling of iodinated deoxycytidine…”
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13
Cyanine-dye-modified 2′-deoxyuridine-5′-triphosphates: Synthesis, applications, and linker effect on substrate properties for Taq DNA polymerase
Published in Russian journal of bioorganic chemistry (01-07-2017)“…A number of fluorescently labeled nucleoside triphosphates containing electroneutral indodicarbocyanine dye (Cy) have been synthesized. The dye has been…”
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14
The Effect of the Chromophore Charge on the Efficiency of Incorporation of Fluorescently-labeled Nucleotides in Matrix Synthesis by Taq DNA Polymerase
Published in Biofizika (01-11-2015)“…In order to study the effect of an electrical charge of the chromophore, on the efficiency of incorporation of fluorescently-labeled nucleotides into DNA…”
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15
Generation of Active Carboxyl Groups on the Surface of a Polyethylene Terephthalate Film and Their Quantitation by Digital Fluorescence Microscopy
Published in Biophysics (Oxford) (01-07-2018)“…A method to estimate the concentration of chemically accessible carboxylic groups on the surface of a polyethylene terephthalate (PET) film was developed using…”
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16
The Effect of the Structure of Fluorescently Labeled Nucleotide Derivatives on the Efficiency of Their Incorporation in DNA in the Polymerase Chain Reaction
Published in Biophysics (Oxford) (01-11-2017)“…The efficiency of fluorescence DNA labeling was estimated for four fluorescent 2′-deoxyuridine 5′-triphosphate derivatives differing in the orientation of the…”
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17
The kinetics of fluorescent DNA labeling using PCR with different Taq polymerases depends on the chemical structures of modified nucleotides
Published in Biophysics (Oxford) (01-05-2017)“…The kinetics of DNA labeling during PCR using six fluorescent derivatives of 2′-deoxyuridine 5′-triphosphate has been studied. These compounds differ in their…”
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18
The effect of chromophore charge on the incorporation efficiency of fluorescence-labeled nucleotides catalyzed by Taq DNA polymerase in matrix synthesis
Published in Biophysics (Oxford) (01-11-2015)“…The effect of the chromophore charge on the efficiency of incorporation of fluorescent-labeled nucleotides into DNA during PCR was studied using three dUTP…”
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19
Preparation of Modified Combinatorial DNA Libraries via Emulsion PCR with Subsequent Strand Separation
Published in Molekuliarnaia biologiia (01-11-2018)“…A modification of the enzymatic method for the preparation of combinatorial random DNA libraries, which combines amplification in isolated microvolumes with…”
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Journal Article -
20
Enzymatic synthesis of high-modified DNA
Published in Molekuliarnaia biologiia (01-05-2017)“…Here, we describe the synthesis and purification of six deoxyuridine triphosphate derivatives that contain protein-like functional groups and alkene linkers of…”
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