Search Results - "Gurumurthy, Channabasavaiah"

Easi-CRISPR for creating knock-in and conditional knockout mouse models using long ssDNA donors by Miura, Hiromi, Quadros, Rolen M, Gurumurthy, Channabasavaiah B, Ohtsuka, Masato

“…This protocol describes Easi -CRISPR, a method for creating knock-in, conditional knockout, and knockdown mouse models by CRISPR/Cas9-based genome engineering…”
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Generating mouse models for biomedical research: technological advances by Gurumurthy, Channabasavaiah B, Lloyd, Kevin C Kent

“…Over the past decade, new methods and procedures have been developed to generate genetically engineered mouse models of human disease. This At a Glance article…”
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Cross-contamination of CRISPR guides and other unrelated nucleotide sequences among commercial oligonucleotides by Arakawa, Hiroshi, Miura, Hiromi, Quadros, Rolen M, Ohtsuka, Masato, Gurumurthy, Channabasavaiah B

“…Custom oligonucleotides (oligos) are widely used reagents in biomedical research. Some common applications of oligos include polymerase chain reaction (PCR),…”
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Diagnostics for SARS-CoV-2 infections by Kevadiya, Bhavesh D., Machhi, Jatin, Herskovitz, Jonathan, Oleynikov, Maxim D., Blomberg, Wilson R., Bajwa, Neha, Soni, Dhruvkumar, Das, Srijanee, Hasan, Mahmudul, Patel, Milankumar, Senan, Ahmed M., Gorantla, Santhi, McMillan, JoEllyn, Edagwa, Benson, Eisenberg, Robert, Gurumurthy, Channabasavaiah B., Reid, St Patrick M., Punyadeera, Chamindie, Chang, Linda, Gendelman, Howard E.

“…Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has spread to nearly every corner of the globe, causing societal instability. The resultant…”
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Arid1b haploinsufficiency disrupts cortical interneuron development and mouse behavior by Jung, Eui-Man, Moffat, Jeffrey Jay, Liu, Jinxu, Dravid, Shashank Manohar, Gurumurthy, Channabasavaiah Basavaraju, Kim, Woo-Yang

“…Haploinsufficiency of the AT-rich interactive domain 1B ( ARID1B ) gene causes autism spectrum disorder and intellectual disability; however, the…”
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CRISPR/Cas9-based generation of knockdown mice by intronic insertion of artificial microRNA using longer single-stranded DNA by Miura, Hiromi, Gurumurthy, Channabasavaiah B, Sato, Takehito, Sato, Masahiro, Ohtsuka, Masato

“…Knockdown mouse models, where gene dosages can be modulated, provide valuable insights into gene function. Typically, such models are generated by embryonic…”
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i-GONAD: a robust method for in situ germline genome engineering using CRISPR nucleases by Ohtsuka, Masato, Sato, Masahiro, Miura, Hiromi, Takabayashi, Shuji, Matsuyama, Makoto, Koyano, Takayuki, Arifin, Naomi, Nakamura, Shingo, Wada, Kenta, Gurumurthy, Channabasavaiah B

“…We present a robust method called improved-Genome editing via Oviductal Nucleic Acids Delivery (i-GONAD) that delivers CRISPR ribonucleoproteins to E0.7…”
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Easi-CRISPR: a robust method for one-step generation of mice carrying conditional and insertion alleles using long ssDNA donors and CRISPR ribonucleoproteins by Quadros, Rolen M, Miura, Hiromi, Harms, Donald W, Akatsuka, Hisako, Sato, Takehito, Aida, Tomomi, Redder, Ronald, Richardson, Guy P, Inagaki, Yutaka, Sakai, Daisuke, Buckley, Shannon M, Seshacharyulu, Parthasarathy, Batra, Surinder K, Behlke, Mark A, Zeiner, Sarah A, Jacobi, Ashley M, Izu, Yayoi, Thoreson, Wallace B, Urness, Lisa D, Mansour, Suzanne L, Ohtsuka, Masato, Gurumurthy, Channabasavaiah B

“…Conditional knockout mice and transgenic mice expressing recombinases, reporters, and inducible transcriptional activators are key for many genetic studies and…”
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Prototype mouse models for researching SEND-based mRNA delivery and gene therapy by Gurumurthy, Channabasavaiah B., Quadros, Rolen M., Ohtsuka, Masato

“…One of the major challenges of gene therapy—an approach to treat diseases caused by faulty genes—is a lack of technologies that deliver healthy gene copies to…”
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Creation of CRISPR-based germline-genome-engineered mice without ex vivo handling of zygotes by i-GONAD by Gurumurthy, Channabasavaiah B., Sato, Masahiro, Nakamura, Ayaka, Inui, Masafumi, Kawano, Natsuko, Islam, Md Atiqul, Ogiwara, Sanae, Takabayashi, Shuji, Matsuyama, Makoto, Nakagawa, Shinichi, Miura, Hiromi, Ohtsuka, Masato

“…Methods to create genetically engineered mice involve three major steps: harvesting embryos from one set of females, microinjection of reagents into embryos ex…”
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Cleavage by Caspase 8 and Mitochondrial Membrane Association Activate the BH3-only Protein Bid during TRAIL-induced Apoptosis by Huang, Kai, Zhang, Jingjing, O’Neill, Katelyn L., Gurumurthy, Channabasavaiah B., Quadros, Rolen M., Tu, Yaping, Luo, Xu

“…The BH3-only protein Bid is known as a critical mediator of the mitochondrial pathway of apoptosis following death receptor activation. However, since…”
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Human genome-edited hematopoietic stem cells phenotypically correct Mucopolysaccharidosis type I by Gomez-Ospina, Natalia, Scharenberg, Samantha G., Mostrel, Nathalie, Bak, Rasmus O., Mantri, Sruthi, Quadros, Rolen M., Gurumurthy, Channabasavaiah B., Lee, Ciaran, Bao, Gang, Suarez, Carlos J., Khan, Shaukat, Sawamoto, Kazuki, Tomatsu, Shunji, Raj, Nitin, Attardi, Laura D., Aurelian, Laure, Porteus, Matthew H.

“…Lysosomal enzyme deficiencies comprise a large group of genetic disorders that generally lack effective treatments. A potential treatment approach is to…”
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GONAD: Genome-editing via Oviductal Nucleic Acids Delivery system: a novel microinjection independent genome engineering method in mice by Takahashi, Gou, Gurumurthy, Channabasavaiah B, Wada, Kenta, Miura, Hiromi, Sato, Masahiro, Ohtsuka, Masato

“…Microinjection is considered the gold standard technique for delivery of nucleic acids (NAs; transgenes or genome editing tools such as CRISPR/Cas9 systems)…”
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Genetically modified mouse models to help fight COVID-19 by Gurumurthy, Channabasavaiah B., Quadros, Rolen M., Richardson, Guy P., Poluektova, Larisa Y., Mansour, Suzanne L., Ohtsuka, Masato

“…The research community is in a race to understand the molecular mechanisms of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, to…”
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Precise and efficient nucleotide substitution near genomic nick via noncanonical homology-directed repair by Nakajima, Kazuhiro, Zhou, Yue, Tomita, Akiko, Hirade, Yoshihiro, Gurumurthy, Channabasavaiah B, Nakada, Shinichiro

“…CRISPR/Cas9, which generates DNA double-strand breaks (DSBs) at target loci, is a powerful tool for editing genomes when codelivered with a donor DNA template…”
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CD4+ effector T cells accelerate Alzheimer's disease in mice by Machhi, Jatin, Yeapuri, Pravin, Lu, Yaman, Foster, Emma, Chikhale, Rupesh, Herskovitz, Jonathan, Namminga, Krista L, Olson, Katherine E, Abdelmoaty, Mai Mohamed, Gao, Ju, Quadros, Rolen M, Kiyota, Tomomi, Jingjing, Liang, Kevadiya, Bhavesh D, Wang, Xinglong, Liu, Yutong, Poluektova, Larisa Y, Gurumurthy, Channabasavaiah B, Mosley, R Lee, Gendelman, Howard E

“…Alzheimer's disease (AD) is a progressive neurodegenerative disorder characterized by pathological deposition of misfolded self-protein amyloid beta (Aβ) which…”
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Simplified CRISPR tools for efficient genome editing and streamlined protocols for their delivery into mammalian cells and mouse zygotes by Jacobi, Ashley M., Rettig, Garrett R., Turk, Rolf, Collingwood, Michael A., Zeiner, Sarah A., Quadros, Rolen M., Harms, Donald W., Bonthuis, Paul J., Gregg, Christopher, Ohtsuka, Masato, Gurumurthy, Channabasavaiah B., Behlke, Mark A.

“…[Display omitted] •Efficient genome editing using crRNA, tracrRNA and Cas9 ribonucleoproteins (ctRNPs).•Delivery of CRISPR ctRNPs into mammalian cells via…”
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Knock-in mouse models for studying somatostatin and cholecystokinin expressing cells by Balog, Marta, Anderson, Allison, Gurumurthy, Channabasavaiah B., Quadros, Rolen M., Korade, Zeljka, Mirnics, Karoly

“…Somatostatin (SST) and cholecystokinin (CCK) are peptide hormones that regulate the endocrine system, cell proliferation and neurotransmission. We utilized the…”
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Improved Genome Editing via Oviductal Nucleic Acids Delivery (i-GONAD): Protocol Steps and Additional Notes by Sato, Masahiro, Nakamura, Ayaka, Sekiguchi, Marie, Matsuwaki, Takashi, Miura, Hiromi, Gurumurthy, Channabasavaiah B, Kakuta, Shigeru, Ohtsuka, Masato

“…The clustered regularly interspaced short palindromic repeats (CRISPR) technology has made it possible to produce genome-edited (GE) animals more easily and…”
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Rescue of auditory function by a single administration of AAV-TMPRSS3 gene therapy in aged mice of human recessive deafness DFNB8 by Du, Wan, Ergin, Volkan, Loeb, Corena, Huang, Mingqian, Silver, Stewart, Armstrong, Ariel Miura, Huang, Zaohua, Gurumurthy, Channabasavaiah B., Staecker, Hinrich, Liu, Xuezhong, Chen, Zheng-Yi

“…Patients with mutations in the TMPRSS3 gene suffer from recessive deafness DFNB8/DFNB10. For these patients, cochlear implantation is the only treatment…”
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