Search Results - "Gupta, Munishwar N."
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Lipase catalyzed preparation of biodiesel from Jatropha oil in a solvent free system
Published in Process biochemistry (1991) (01-03-2007)“…The monoethyl esters of the long chain fatty acids (biodiesel) were prepared by alcoholysis of Jatropha oil, a non-edible oil, by a lipase. The process…”
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Preparation of cross-linked enzyme aggregates by using bovine serum albumin as a proteic feeder
Published in Analytical biochemistry (15-04-2006)“…Addition of bovine serum albumin (BSA) as a proteic feeder facilitates obtaining cross-linked enzyme aggregates (CLEAs) in cases where the protein…”
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Preparation of Biodiesel by Lipase-Catalyzed Transesterification of High Free Fatty Acid Containing Oil from Madhuca indica
Published in Energy & fuels (01-01-2007)“…The oil from Madhuca indica has a high free fatty acid content, and hence, it is difficult to convert it into biodiesel by using a chemical catalyst. In this…”
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Kinetic resolution of (±)-1-phenylethanol in [Bmim][PF 6] using high activity preparations of lipases
Published in Bioorganic & medicinal chemistry letters (15-02-2007)“…It is shown that high activity preparations of the lipases are far superior to the lyophilized powders in kinetic resolution of (±)-1-phenylethanol in…”
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Lipase catalyzed transesterification of castor oil by straight chain higher alcohols
Published in Journal of bioscience and bioengineering (01-03-2015)“…Biolubricants from Castor oil were produced enzymatically by transesterification with higher alcohols using a lipase mixture of immobilized Mucor miehei (RMIM)…”
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Immobilization of a Lipase on Mesocellular Foam of Silica for Biocatalysis in Low-water-containing Organic Solvents
Published in Chemistry letters (2014)“…Unfunctionalized mesocellular foam of silica was used for immobilization of the lipase from Thermomyces lanuginosus. In the first approach, lipase was adsorbed…”
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Preparation and characterization of combi-CLEAs catalyzing multiple non-cascade reactions
Published in Journal of molecular catalysis. B, Enzymatic (01-03-2007)“…A novel cross-linked enzyme aggregates (CLEA) concept called combi-CLEA has been described. It is based upon the fact that CLEA can be made from heterogeneous…”
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A novel α-L-arabinofuranosidase of family 43 glycoside hydrolase (Ct43Araf) from Clostridium thermocellum
Published in PloS one (09-09-2013)“…The study describes a comparative analysis of biochemical, structural and functional properties of two recombinant derivatives from Clostridium thermocellum…”
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Effectiveness of cross-linked enzyme aggregates of cellulolytic enzymes in hydrolyzing wheat straw
Published in Journal of bioscience and bioengineering (01-10-2018)“…Development of industrially potent cellulolytic enzymes is one of the greatest challenges faced in lignocellulosic feed-stock based bio-refining. In the…”
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The family 6 Carbohydrate Binding Module (CtCBM6) of glucuronoxylanase (CtXynGH30) of Clostridium thermocellum binds decorated and undecorated xylans through cleft A
Published in Archives of biochemistry and biophysics (01-06-2015)“…[Display omitted] •CtCBM6 has two potential binding clefts, cleft A and cleft B.•Cleft A key residues are Tyr28, Phe84 and Asn112.•Cleft B key residues Tyr34…”
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Protein promiscuity in drug discovery, drug-repurposing and antibiotic resistance
Published in Biochimie (01-08-2020)“…Proteins are supposed to bind to their substrates/ligands in a specific manner via their pre-formed binding sites, according to classical biochemistry. In…”
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Structural Modelling, Substrate Binding and Stability Studies of Endopectate Lyase (PL1B) of Family 1 Polysaccharide Lyase from Clostridium thermocellum
Published in Protein and peptide letters (01-01-2015)“…An endo-pectate lyase (PL1B) of family 1 polysaccharide lyase from Clostridium thermocellum was structurally characterized and its stability under chaotropic…”
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Leveraging protein purification strategies in proteomics
Published in Journal of chromatography. B, Analytical technologies in the biomedical and life sciences (15-04-2007)“…The proteomic studies, although, tend to be analytical in nature, yet many strategies of preparative protein purification can be usefully employed in such…”
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Intrinsic disorder in proteins: Relevance to protein assemblies, drug design and host-pathogen interactions
Published in Progress in biophysics and molecular biology (01-10-2020)“…Intrinsic disorder in proteins resulting in considerable variation in structure can lead to multiple functions including multi-specificity and diverse…”
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Cross-Linked Enzyme Aggregates for Applications in Aqueous and Nonaqueous Media
Published in Methods in molecular biology (Clifton, N.J.) (01-01-2017)“…Extensive cross-linking of a precipitate of a protein by a cross-linking reagent (glutaraldehyde has been most commonly used) creates an insoluble enzyme…”
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Role of smart polymers in protein purification and refolding
Published in Bioengineered (01-09-2012)“…Affinity precipitation is a non-chromatographic method which is useful for purification and refolding of proteins. Quite often, a stimuli-sensitive polymer can…”
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Non-chromatographic strategies for protein refolding
Published in Recent patents on biotechnology (01-04-2012)“…Overexpression of recombinant proteins in bacterial systems (such as E. coli) often leads to formation of inactive and insoluble ' inclusion bodies' . Protein…”
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Protein-Coated Microcrystals, Combi-Protein-Coated Microcrystals, and Cross-Linked Protein-Coated Microcrystals of Enzymes for Use in Low-Water Media
Published in Methods in molecular biology (Clifton, N.J.) (01-01-2017)“…Protein-coated microcrystals (PCMC) are a high-activity preparation of enzymes for use in low-water media. The protocols for the preparation of PCMCs of…”
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Stable Cellulolytic Enzymes and Their Application in Hydrolysis of Lignocellulosic Biomass
Published in Biotechnology journal (01-06-2018)“…The effective utilization of lignocellulosic biomass for production of value‐added products in bio‐refineries mainly depends on their hydrolysis into…”
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Solid state fluorescence of proteins in high throughput mode and its applications [version 2; peer review: 2 approved]
Published in F1000 research (2013)“…Direct comparison between fluorescence spectra of a sample in solution and solid state form is valuable to monitor the changes in protein structure when it is…”
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