Search Results - "Gupta, M.N."

Extraction of oil from Jatropha curcas L. seed kernels by enzyme assisted three phase partitioning by Shah, Shweta, Sharma, Aparna, Gupta, M.N.

“…Three phase partitioning has been evaluated for extraction of oil from Jatropha curcas L. seeds. This process consisted of simultaneous addition of t-butanol…”
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Ultrasonic pre-irradiation effect upon aqueous enzymatic oil extraction from almond and apricot seeds by Sharma, Aparna, Gupta, M.N.

“…The present work evaluates the benefit of using ultrasonic pre-irradiation before extracting oil from almond and apricot seeds by aqueous enzymatic oil…”
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One-step purification and characterization of an alkaline protease from haloalkaliphilic Bacillus sp by Gupta, Anshu, Roy, Ipsita, Patel, R.K., Singh, S.P., Khare, S.K., Gupta, M.N.

“…An alkaline protease producer haloalkaliphilic bacteria (isolate Vel) was isolated from west coast of India. It was related to Bacillus pseudofirmus on the…”
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Purification and characterization of a solvent stable protease from Pseudomonas aeruginosa PseA by Gupta, Anshu, Roy, Ipsita, Khare, S.K., Gupta, M.N.

“…A solvent tolerant Pseudomonas aeruginosa PseA strain was isolated from soil. It secreted a novel alkaline protease, which was stable and active in the…”
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Three phase partitioning as a large-scale separation method for purification of a wheat germ bifunctional protease/amylase inhibitor by Sharma, Aparna, Gupta, M.N.

“…The technique of three phase partitioning (TPP) was used to purify a wheat germ bifunctional protease/amylase inhibitor. TPP is a relatively recent technique,…”
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Three phase partitioning of carbohydrate polymers: separation and purification of alginates by Sharma, A, Gupta, M.N

“…Three phase partitioning, a technique described for protein purification, has been employed for precipitation and purification of three different commercial…”
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Accelerating Enzymatic Hydrolysis of Chitin by Microwave Pretreatment by Roy, Ipsita, Mondal, Kalyani, Gupta, Munishwar N.

“…Response surface analysis was used to determine optimum conditions [2% (w/v) chitin, 57.5 °C, 38 min] for microwave irradiation of chitin to improve its…”
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Polarity Index: The Guiding Solvent Parameter for Enzyme Stability in Aqueous-Organic Cosolvent Mixtures by Gupta, Munishwar N., Batra, Renu, Tyagi, Renu, Sharma, Aparna

“…Enzyme catalysis in aqueous–organic cosolvent mixtures has wide applications. However, inadequate attention has been paid to the issue of stability of enzymes…”
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Major Antifungal Activity from the Bulbs of Indian Squill Urginea indica Is a Chitinase by Shenoy, S.R, Kameshwari, M.N.S, Swaminathan, S, Gupta, M.N

“…We have identified a chitinase with antifungal activity in the bulbs of the plant Urginea indica(Indian squill) and purified it about 26‐fold. The purified…”
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Enhancement of enzyme activity through three-phase partitioning: crystal structure of a modified serine proteinase at 1.5 Å resolution by Singh, R.K., Gourinath, S., Sharma, S., Roy, I., Gupta, M.N., Betzel, Ch, Srinivasan, A., Singh, T.P.

“…Three-phase partitioning is fast developing as a novel bioseparation strategy with a wide range of applications including enzyme stability and enhancement of…”
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Hydrolysis of rice hull by crosslinked Aspergillus niger cellulase by Sharma, A, Khare, S.K, Gupta, M.N

“…A.niger cellulase was crosslinked by glutaraldehyde to obtain a heat-stable enzyme preparation for rice hull cellulose hydrolysis. Under optimized crosslinking…”
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Integration of Affinity Precipitation with Partitioning Methods for Bioseparation of Chitin Binding Lectins by Teotia, S., Mondal, K., Gupta, M.N.

“…The present work describes the applications of affinity precipitation and aqueous two-phase affinity extraction (ATPAE) for purification of lectins from wheat…”
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Evaluation of microbeads of calcium alginate as a fluidized bed medium for affinity chromatography of Aspergillus niger pectinase by Roy, I, Jain, S, Teotia, S, Gupta, M.N

“…Calcium alginate microbeads (212–425 μm) were prepared by spraying 2% (w/v) alginate solution into 1 M CaCl2 solution. The fluidization behavior of these beads…”
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Noncovalent immobilization of enzymes on an enteric polymer Eudragit S-100 by Sardar, M., Agarwal, R., Kumar, A., Gupta, M.N.

“…The noncovalent immobilization of enzymes such as alpha-amylase, beta-glucosidase, trypsin, and alkaline phosphatase was performed by adsorption on the…”
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pH-responsive polymer-assisted refolding of urea- and organic solvent-denatured  -chymotrypsin by Roy, I., Gupta, M.N.

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Medical implications of protein moonlighting by Gupta, M, Pandey, Saurabh, Ehtesham, Nasreen, Hasnain, Seyed

“…Almost all drugs essentially act by interaction with proteins by modulating their activities. [...]recently, it was believed that this specificity was critical…”
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Biodiesel Preparation by Lipase-Catalyzed Transesterification of Jatropha Oil by Shah, Shweta, Sharma, Shweta, Gupta, M. N

“…Alkyl esters of long chain fatty acid are called biodiesel. These esters can be obtained from vegetable oils by transesterification with methanol/ethanol. The…”
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Extraction of oil from Jatropha curcas L. seed kernels by combination of ultrasonication and aqueous enzymatic oil extraction by Shah, Shweta, Sharma, Aparna, Gupta, M.N.

“…Use of ultrasonication as a pretreatment before aqueous oil extraction and aqueous enzymatic oil extraction was found to be useful in the case of extraction of…”
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Single-step purification of recombinant green fluorescent protein on expanded beds of immobilized metal affinity chromatography media by Dalal, Sohel, Raghava, Smita, Gupta, M.N.

“…Immobilized metal ion affinity chromatography (IMAC) in expanded bed mode is used for purifying recombinant green fluorescent protein (GFP) overexpressed in…”
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Hydrolysis of starch by a mixture of glucoamylase and pullulanase entrapped individually in calcium alginate beads by Roy, Ipsita, Gupta, Munishwar Nath

“…Hydrolysis of starch was carried out by a blend of individually entrapped glucoamylase and pullulanase (in calcium alginate beads) in the ratio of 3:2…”
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