A Functional Assay for the Determination of Heparin-Induced Thrombocytopenia via Flow Cytometry

A Functional Assay for the Determination of Heparin-Induced Thrombocytopenia via Flow Cytometry

Heparin-induced thrombocytopenia (HIT) is a life-threatening complication of heparin therapy (both unfractionated heparin and low-molecular-weight heparin). In our study, we examined a group of 122 patients with suspected HIT. The samples of all patients were analyzed in the first step using an immu...

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Published in:Diagnostics (Basel) Vol. 13; no. 18; p. 3019
Main Authors: Skornova, Ingrid, Simurda, Tomas, Stanciakova, Lucia, Lauko, Viliam, Holly, Pavol, Samos, Matej, Bolek, Tomas, Schnierer, Martin, Drotarova, Miroslava, Belakova, Kristina Maria, Sokol, Juraj, Stasko, Jan, Mokan, Marian, Gumulec, Jaroslav, Chrastinova, Leona
Format: Journal Article
Language:English
Published: Basel MDPI AG 01-09-2023
MDPI
Subjects:
Analysis
Antibodies
Anticoagulants
Blood platelets
Ethylenediaminetetraacetic acid
Flow cytometry
Hematology
Heparin
heparin-induced thrombocytopenia (HIT)
heparin–PF4 complex (platelet factor 4)
Immunoassay
Immunoglobulin G
immunological and functional assays
Immunology
Laboratories
Patients
Plasma
Serotonin
serotonin release assay (SRA)
Sodium
Thrombocytopenia
Czech Republic
Slovakia
Switzerland
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Summary:Heparin-induced thrombocytopenia (HIT) is a life-threatening complication of heparin therapy (both unfractionated heparin and low-molecular-weight heparin). In our study, we examined a group of 122 patients with suspected HIT. The samples of all patients were analyzed in the first step using an immunoassay (ID-PaGIA Heparin/PF4, Hemos1L-Acustar HIT IgG, ZYMUTEST HIA Monostrip IgG) to detect the presence of antibodies against heparin–PF4 complexes (platelet factor 4). When the immunoassay was positive, the sample was subsequently analyzed for HIT with a functional flow cytometry assay, the HITAlert kit, the purpose of which was to demonstrate the ability of the antibodies present to activate platelets. A diagnosis of HIT can be made only after a positive functional test result. In this article, we present an overview of our practical experience with the use of the new functional method of analysis, HIT, with flow cytometry. In this work, we compared the mutual sensitivity of two functional tests, SRA and the flow cytometry HITAlert kit, in patients perceived as being at risk for HIT. This work aims to delineate the principle, procedure, advantages, pitfalls, and possibilities of the application of the functional test HITAlert using flow cytometry.
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ISSN:2075-4418
2075-4418
DOI:10.3390/diagnostics13183019