Isolation, Identification and High-Throughput Screening of Neutral Lipid Producing Indigenous Microalgae from South African Aquatic Habitats

Exploring indigenous microalgae capable of producing significant amounts of neutral lipids through high-throughput screening is crucial for sustainable biodiesel production. In this study, 31 indigenous microalgal strains were isolated from diverse aquatic habitats in KwaZulu-Natal, South Africa. Ei...

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Bibliographic Details
Published in:	Applied biochemistry and biotechnology Vol. 182; no. 1; pp. 382 - 399
Main Authors:	Gumbi, S. T., Majeke, B. M., Olaniran, A. O., Mutanda, T.
Format:	Journal Article
Language:	English
Published:	New York Springer US 01-05-2017 Springer Nature B.V
Subjects:	Algae Aquatic habitats Aquatic Organisms Biochemistry Biodiesel fuels Biofuels Biomass Biotechnology Chemistry Chemistry and Materials Science Chlamydomonas - chemistry Chlamydomonas - genetics Chlamydomonas - metabolism Chlorella Chlorella - chemistry Chlorella - genetics Chlorella - metabolism Chlorella vulgaris Diesel Fatty acids Fatty Acids - biosynthesis Fatty Acids - isolation & purification Fatty Acids, Monounsaturated - isolation & purification Fatty Acids, Monounsaturated - metabolism Fatty Acids, Unsaturated - biosynthesis Fatty Acids, Unsaturated - isolation & purification Fluorescence Fluorescence microscopy Gene sequencing Genera Gravimetric analysis Growth rate High-throughput screening High-Throughput Screening Assays Lipids Microalgae Microalgae - chemistry Microalgae - genetics Microalgae - metabolism Polyunsaturated fatty acids RNA, Ribosomal, 18S - genetics rRNA 18S Screening South Africa Sustainable production Vanillin GC-MS Indigenous Lipid profile Bioprospect Microalgae Biodiesel sp Fatty acid methyl esters Chlorella sp
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Summary:	Exploring indigenous microalgae capable of producing significant amounts of neutral lipids through high-throughput screening is crucial for sustainable biodiesel production. In this study, 31 indigenous microalgal strains were isolated from diverse aquatic habitats in KwaZulu-Natal, South Africa. Eight superior lipid-producing strains were selected for further analysis, based on Nile red fluorescence microscopy screening. The microalgal isolates were identified to belong to the genera Chlorella , Neochloris and Chlamydomonas via morpho-taxonomic and molecular approach by 18S rRNA gene sequencing. Chlorella vulgaris PH2 had the highest specific growth rate ( μ ) and lowest doubling time of 0.24 day −1 and 2.89 ± 0.05 day −1 , respectively. Chlorella vulgaris T4 had the highest biomass productivity of 35.71 ± 0.03 mg L −1 day −1 . Chlorella vulgaris PH2 had the highest lipid content of 34.28 ± 0.47 and 38 ± 9.2% (dcw) as determined by gravimetric analysis and the sulfo-phospho-vanillin (SPV) method, respectively. Chlorella vulgaris PH2 exhibited a high content of saturated fatty acids, while Chlorella sp. T4 exhibited a high total content of saturated and monounsaturated fatty acids with a low content of polyunsaturated fatty acids. The preponderance of neutral lipids suggests that Chlorella sp. T4 is a suitable candidate for biomass feedstock for biodiesel production.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0273-2289 1559-0291
DOI:	10.1007/s12010-016-2333-z