A novel variant of DR11 (DRB11128) identified in the family by polymerase chain reaction-reverse dot blot

A novel variant of DR11 (DRB11128) identified in the family by polymerase chain reaction-reverse dot blot

The HLA class II DRB1 locus currently encodes at least 135 alleles (1) that can be detected by using a variety of molecular biology techniques, such as PCR-SSO, PCR-SSP and PCR-RFLP. Using a commercially available PCR reverse dot-blot hybridization assay (INNO-LiPA) from Innogenetics, Belgium, we id...

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Bibliographic Details
Published in:Tissue antigens Vol. 50; no. 1; pp. 94 - 95
Main Authors: Gulgnler, F., Dufossé, F., Cracco, P., Krausé, C., Coffe, C.
Format: Journal Article
Language:English
Published: Oxford, UK Blackwell Publishing Ltd 01-07-1997
Subjects:
Base Sequence
DNA
DNA sequencing
Female
Genetic Variation
HLA DRB1 allele
HLA-DR Antigens - genetics
HLA-DR Serological Subtypes
Humans
Male
Molecular Sequence Data
Pedigree
Polymerase Chain Reaction
reverse dot blot hybridization
Sequence Homology, Nucleic Acid
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Summary:The HLA class II DRB1 locus currently encodes at least 135 alleles (1) that can be detected by using a variety of molecular biology techniques, such as PCR-SSO, PCR-SSP and PCR-RFLP. Using a commercially available PCR reverse dot-blot hybridization assay (INNO-LiPA) from Innogenetics, Belgium, we identified one additional DRB1*11 allele in a Causasoid bone marrow volunteer and her family named DRB1*1128 that has an identical sequence to DRB1*11011 except at the location of codon 37. Genomic DNA was isolated from blood using a salting-out method HLA DRB1, DQB1 and DPB1 were performed by using the INNO-LiPA kits according to Buyse et al., and additional typing was carried out using PCR-SSP. Direct DNA sequencing was performed according to the method described by Sanger et al.
Bibliography:ArticleID:TAN94
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ISSN:0001-2815
1399-0039
DOI:10.1111/j.1399-0039.1997.tb02844.x