NMR study of the interaction of P-450 with 4-methoxypyridine

NMR study of the interaction of P-450 with 4-methoxypyridine

Longitudinal relaxation (T 1) measurements for all lines (α-CH, β-CH, O-CH 3) in the 4-methoxypyridine 1H-NMR spectrum were used to study the interaction of 4-methoxypyridine with purified microsomal cytochrome P-450 from livers of phenobarbital-treated rats. The paramagnetic contribution to the obs...

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Bibliographic Details
Published in:FEBS letters Vol. 212; no. 1; pp. 53 - 57
Main Authors: Woldman, Ya.Yu, Weiner, L.M., Gulayeva, L.F., Lyakhovich, V.V.
Format: Journal Article
Language:English
Published: Amsterdam Elsevier B.V 09-02-1987
Elsevier
Subjects:
1H-NMR
4-Methoxypyridine
Animals
Biological and medical sciences
Carbon Monoxide - metabolism
Cytochrome P-450
Cytochrome P-450 Enzyme System - metabolism
cytochrome P450
Enzyme-substrate interaction
Fundamental and applied biological sciences. Psychology
Interactions. Associations
Intermolecular phenomena
Magnetic Resonance Spectroscopy
Microsomes, Liver - enzymology
Molecular biophysics
N.M.R
Pyridines - metabolism
Rats
Enzyme-substrate interaction
1H-NMR
4-Methoxypyridine
Cytochrome P-450
Vertebrata
Hemoprotein
Mammalia
Rat
Magnetic relaxation
Enzyme
Cytochrome P450
Liver
Rodentia
Molecular association
NMR spectrometry
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Summary:Longitudinal relaxation (T 1) measurements for all lines (α-CH, β-CH, O-CH 3) in the 4-methoxypyridine 1H-NMR spectrum were used to study the interaction of 4-methoxypyridine with purified microsomal cytochrome P-450 from livers of phenobarbital-treated rats. The paramagnetic contribution to the observed T 1 −1 value was determined from its dependence on 4-methoxypyridine concentration. In the P-450-4-methoxypyridine complex the latter is oriented so that the nitrogen of pyridine is directed towards the Fe 3+.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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content type line 23
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(87)81555-7