Calcitonin gene-related peptide receptor expression in healthy and inflamed human pulp tissue

Aim To use radioreceptor analysis for comparing calcitonin gene‐related peptide (CGRP) receptor expression in human pulp tissue samples collected from teeth having a clinical diagnosis of acute irreversible pulpitis, healthy pulps and teeth with induced inflammation. Methodology Six pulp samples w...

Full description

Saved in:

Bibliographic Details
Published in:	International endodontic journal Vol. 38; no. 10; pp. 712 - 717
Main Authors:	Caviedes-Bucheli, J., Arenas, N., Guiza, O., Moncada, N. A., Moreno, G. C., Diaz, E., Munoz, H. R.
Format:	Journal Article
Language:	English
Published:	Oxford, UK Blackwell Science Ltd 01-10-2005
Subjects:	Adult Binding Sites calcitonin gene-related peptide receptor Dental Pulp - metabolism Dentistry human pulp Humans Iodine Radioisotopes neurogenic inflammation Neurogenic Inflammation - metabolism Pulpitis - metabolism Radioligand Assay radioreceptor assay Receptors, Calcitonin Gene-Related Peptide - analysis Receptors, Calcitonin Gene-Related Peptide - biosynthesis Statistics, Nonparametric
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Aim To use radioreceptor analysis for comparing calcitonin gene‐related peptide (CGRP) receptor expression in human pulp tissue samples collected from teeth having a clinical diagnosis of acute irreversible pulpitis, healthy pulps and teeth with induced inflammation. Methodology Six pulp samples were obtained from teeth having a clinical diagnosis of acute irreversible pulpitis. Another eight pulp samples were obtained from healthy premolars where extraction was indicated for orthodontic purposes. In four of these premolars, inflammation was induced prior to pulp collection. All the samples were processed and labelled with 125I‐CGRP. Binding sites were identified by 125I‐CGRP and standard CGRP competition assays. Results CGRP receptor expression was found in all human pulp tissue samples. Most receptors were found in the group of pulps from teeth having a clinical diagnosis of acute irreversible pulpitis, followed by the group of pulps having induced inflammation. The least number of receptors was expressed in the group of healthy pulps. The Kruskal–Wallis and Mann–Whitney (post‐hoc) tests showed statistically significant differences between the groups (P < 0.05). Conclusion CGRP receptor expression in human pulp tissue is significantly increased during inflammatory phenomena such as acute irreversible pulpitis.
Bibliography:	istex:347F39C73BBFD815C5CEBA685F269F8CF49C4342 ArticleID:IEJ1006 ark:/67375/WNG-NRTLC814-P ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0143-2885 1365-2591
DOI:	10.1111/j.1365-2591.2005.01006.x