A new source of (R)-limonene and rotundifolone from leaves of Lippia pedunculosa (verbenaceae) and their trypanocidal properties

Investigation by GC-FID and GC-MS of the essential oil (LPOE) from the leaves of Lippia pedunculosa revealed, as the major compounds, the monoterpenes rotundifolone (71.7%) and (R)-limonene (21.8%). These two compounds and the minor constituent piperitenone (1.2%) were also isolated from the leaves...

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Bibliographic Details
Published in:	Natural product communications Vol. 9; no. 6; p. 737
Main Authors:	Santos, Nilmara Nunes, Menezes, Leociley Rocha Alencar, dos Santos, Jamyle Andrade Ferreira, Meira, Cássio Santana, Guimarhes, Elisalva Teixeira, Soares, Milena Botelho Pereira, Nepel, Angelita, Barisone, Andersson, Costa, Emmanoel Vilaça
Format:	Journal Article
Language:	English
Published:	United States 01-06-2014
Subjects:	Animals Cyclohexenes - chemistry Cyclohexenes - pharmacology Lippia - chemistry Macrophages, Peritoneal - parasitology Mice Mice, Inbred BALB C Molecular Structure Monoterpenes - chemistry Monoterpenes - pharmacology Oils, Volatile - chemistry Oils, Volatile - pharmacology Plant Leaves - chemistry Plant Oils - chemistry Plant Oils - pharmacology Terpenes - chemistry Terpenes - pharmacology Trypanocidal Agents - chemistry Trypanocidal Agents - pharmacology Trypanosoma cruzi - drug effects
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Summary:	Investigation by GC-FID and GC-MS of the essential oil (LPOE) from the leaves of Lippia pedunculosa revealed, as the major compounds, the monoterpenes rotundifolone (71.7%) and (R)-limonene (21.8%). These two compounds and the minor constituent piperitenone (1.2%) were also isolated from the leaves and identified by spectrometric analysis. LPOE and isolated compounds were evaluated for their trypanocidal activity against epimastigote and trypomastigote forms of Trypanosoma cruzi. Significant results with IC50 values lower than 34.0 microg.mL(-1) were observed against these forms of T. cruzi for LPOE and isolated compounds. Rotundifolone was the most active compound with an ICso lower than 10.0 ig.mL"' for both forms of T. cruzi. The effects of LPOE and isolated compounds were also evaluated in cultures of macrophages infected with T. cruzi. Treatment with (R)-limonene and rotundifolone caused a moderate reduction in the percentage of macrophages infected by T. cruzi and in the number of intracellular parasites at concentrations non-toxic to macrophages.
ISSN:	1934-578X
DOI:	10.1177/1934578X1400900601