Single-unit and polygraphic recordings associated with systemic or local pharmacology: A multi-purpose stereotaxic approach for the awake, anaesthetic-free, and head-restrained rat

In order to avoid any artifactual pharmacological interferences with anaesthetic agents, a procedure has been developed for working on the awake, anaesthetic‐free rat in a head‐restrained condition. It allows, on the same animal and over several consecutive days, single‐unit recordings in combinatio...

Full description

Saved in:

Bibliographic Details
Published in:	Journal of neuroscience research Vol. 61; no. 1; pp. 88 - 100
Main Authors:	Soulière, Fabienne, Urbain, Nadia, Gervasoni, Damien, Schmitt, Patricia, Guillemort, Claude, Fort, Patrice, Renaud, Bernard, Luppi, Pierre Hervé, Chouvet, Guy
Format:	Journal Article
Language:	English
Published:	New York John Wiley & Sons, Inc 01-07-2000 Wiley
Subjects:	Action Potentials - drug effects Action Potentials - physiology Anesthesia Animals Arousal - physiology Conditioning (Psychology) Electroencephalography - instrumentation Electroencephalography - methods Electromyography - instrumentation Electromyography - methods gamma-Aminobutyric Acid - pharmacology Habituation, Psychophysiologic Iontophoresis Life Sciences Locus Coeruleus - physiology Male microiontophoresis micropressure drug applications Neurons and Cognition Rats Rats, Sprague-Dawley Restraint, Physical - instrumentation single-unit recordings stereotaxic head-restraint Stereotaxic Techniques Substantia Nigra - physiology systemic pharmacology unanaesthetized rat vigilance states monitoring
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	In order to avoid any artifactual pharmacological interferences with anaesthetic agents, a procedure has been developed for working on the awake, anaesthetic‐free rat in a head‐restrained condition. It allows, on the same animal and over several consecutive days, single‐unit recordings in combination with systemic or local pharmacology (microiontophoresis or micropressure ejections), as well as monitoring vigilance states via the electroencephalogram and the electromyogram. After the cementing of a special “U”‐shaped device on its skull under general anaesthesia, the animal is progressively habituated to stay daily, for several hours, under a painless corresponding stereotaxic restraint. This system can be easily adapted to different stereotaxic frames and, because of its spatial flexibility for targetting the desired rostrocaudal or lateral positions, allows access to a large number of cerebral structures. Experiments performed on Globus Pallidus, Substantia Nigra, and Locus Coeruleus neurons, combining the different possibilities of this system, are reported. They demonstrate, on the awake anaesthetic‐free head‐restrained rat, and under suitable ethical conditions, the feasibility of single‐unit recordings of identified neurons associated with the study of their pharmacological reactivity after systemic or local drug administrations without any other drug interferences, and in physiologically relevant conditions such as the spontaneous alternance of vigilance states. J. Neurosci. Res. 61:88–100, 2000. © 2000 Wiley‐Liss, Inc.
Bibliography:	Université Claude Bernard Lyon 1 INSERM istex:E1F0C0A7CB1B2A8CFF127F0780C36B004E673C33 Région Rhône-Alpes (programme Neurosciences-Parkinson) CNRS ArticleID:JNR11 ark:/67375/WNG-ZCQLBK4Z-W Patricia Schmitt died in October 1995, at the age of 24. ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0360-4012 1097-4547
DOI:	10.1002/1097-4547(20000701)61:1<88::AID-JNR11>3.0.CO;2-5