Rapid diagnosis of gram negative urinary infections: identification and antimicrobial susceptibility testing in 24 hours

A rapid method for diagnosing urinary tract infections, using identification and antimicrobial susceptibility testing that can be carried out in 24 hours, was devised. The method relies on direct inoculation of diluted urine (1/500) in the API 20 E and API ATB systems. Urine was simultaneously cultu...

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Bibliographic Details
Published in:	Journal of clinical pathology Vol. 39; no. 2; pp. 208 - 211
Main Authors:	Dupeyron, C M, Guillemin, G A, Leluan, G J
Format:	Journal Article
Language:	English
Published:	London BMJ Publishing Group Ltd and Association of Clinical Pathologists 01-02-1986 BMJ BMJ Publishing Group LTD
Subjects:	Anti-Bacterial Agents - pharmacology Bacterial diseases Bacterial diseases of the urinary system Bacteriuria - diagnosis Bacteriuria - microbiology Biological and medical sciences Gram-Negative Bacteria - drug effects Gram-Negative Bacteria - isolation & purification Human bacterial diseases Humans Infectious diseases Medical sciences Methods Microbial Sensitivity Tests Time Factors Human Infection Urine Urinary system disease Bacteriological investigation Gram negative bacteria
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Summary:	A rapid method for diagnosing urinary tract infections, using identification and antimicrobial susceptibility testing that can be carried out in 24 hours, was devised. The method relies on direct inoculation of diluted urine (1/500) in the API 20 E and API ATB systems. Urine was simultaneously cultured on Columbia blood agar and on Drigalski agar to control the purity and for purposes of comparison. The results of this method and those obtained with a conventional method were compared by analysing 1352 urines. The results showed that all of the organisms were correctly identified using the conventional method, and susceptibility testing (rapid method) gave results that agreed with those of the classical method in 94% of cases, with major discrepancies in only 0.08% of cases. The rapid method applies only to monomicrobial infections.
Bibliography:	istex:9FB00445A102F158259D74485B012F179C7BD900 href:jclinpath-39-208.pdf ark:/67375/NVC-Q2R4C6GZ-2 local:jclinpath;39/2/208 PMID:3950044 ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0021-9746 1472-4146
DOI:	10.1136/jcp.39.2.208