Genome-Wide Screening of mRNA Expression in Leprosy Patients

Leprosy, an infectious disease caused by Mycobacterium leprae, affects millions of people worldwide. However, little is known regarding its molecular pathophysiological mechanisms. In this study, a comprehensive assessment of human mRNA was performed on leprosy skin lesions by using DNA chip microar...

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Published in:Frontiers in genetics Vol. 6; p. 334
Main Authors: Belone, Andrea de Faria F, Rosa, Patrícia S, Trombone, Ana P F, Fachin, Luciana R V, Guidella, Cássio C, Ura, Somei, Barreto, Jaison A, Pinilla, Mabel G, de Carvalho, Alex F, Carraro, Dirce M, Soares, Fernando A, Soares, Cleverson T
Format: Journal Article
Language:English
Published: Switzerland Frontiers Media S.A 20-11-2015
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Summary:Leprosy, an infectious disease caused by Mycobacterium leprae, affects millions of people worldwide. However, little is known regarding its molecular pathophysiological mechanisms. In this study, a comprehensive assessment of human mRNA was performed on leprosy skin lesions by using DNA chip microarrays, which included the entire spectrum of the disease along with its reactional states. Sixty-six samples from leprotic lesions (10TT, 10BT, 10BB, 10BL, 4LL, 14R1, and 10R2) and nine skin biopsies from healthy individuals were used as controls (CC) (ages ranged from 06 to 83 years, 48 were male and 29 female). The evaluation identified 1580 differentially expressed mRNAs [Fold Change (FC) ≥ 2.0, p ≤ 0.05] in diseased lesions vs. healthy controls. Some of these genes were observed in all forms of the disease (CD2, CD27, chit1, FA2H, FAM26F, GZMB, MMP9, SLAMF7, UBD) and others were exclusive to reactional forms (Type "1" reaction: GPNMB, IL1B, MICAL2, FOXQ1; Type "2" reaction: AKR1B10, FAM180B, FOXQ1, NNMT, NR1D1, PTX3, TNFRSF25). In literature, these mRNAs have been associated with numerous pathophysiological processes and signaling pathways and are present in a large number of diseases. The role of these mRNAs maybe studied in the context of developing new diagnostic markers and therapeutic targets for leprosy.
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Edited by: Michael Rossbach, Genome Institute of Singapore, Singapore
Reviewed by: Anne Elizabeth Sarver, University of Minnesota, USA; Jianjun Liu, Genome Institute of Singapore, Singapore; Paola De Sessions, Genome Institute of Singapore, Singapore
This article was submitted to RNA, a section of the journal Frontiers in Genetics
ISSN:1664-8021
1664-8021
DOI:10.3389/fgene.2015.00334