Search Results - "Guenther, Dale"

Expanding RNAi therapeutics to extrahepatic tissues with lipophilic conjugates by Brown, Kirk M., Nair, Jayaprakash K., Janas, Maja M., Anglero-Rodriguez, Yesseinia I., Dang, Lan T. H., Peng, Haiyan, Theile, Christopher S., Castellanos-Rizaldos, Elena, Brown, Christopher, Foster, Donald, Kurz, Jeffrey, Allen, Jeffrey, Maganti, Rajanikanth, Li, Jing, Matsuda, Shigeo, Stricos, Matthew, Chickering, Tyler, Jung, Michelle, Wassarman, Kelly, Rollins, Jeff, Woods, Lauren, Kelin, Alex, Guenther, Dale C., Mobley, Melissa W., Petrulis, John, McDougall, Robin, Racie, Timothy, Bombardier, Jessica, Cha, Diana, Agarwal, Saket, Johnson, Lei, Jiang, Yongfeng, Lentini, Scott, Gilbert, Jason, Nguyen, Tuyen, Chigas, Samantha, LeBlanc, Sarah, Poreci, Urjana, Kasper, Anne, Rogers, Arlin B., Chong, Saeho, Davis, Wendell, Sutherland, Jessica E., Castoreno, Adam, Milstein, Stuart, Schlegel, Mark K., Zlatev, Ivan, Charisse, Klaus, Keating, Mark, Manoharan, Muthiah, Fitzgerald, Kevin, Wu, Jing-Tao, Maier, Martin A., Jadhav, Vasant

“…Therapeutics based on short interfering RNAs (siRNAs) delivered to hepatocytes have been approved, but new delivery solutions are needed to target additional…”
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Small circular interfering RNAs (sciRNAs) as a potent therapeutic platform for gene-silencing by Jahns, Hartmut, Degaonkar, Rohan, Podbevsek, Peter, Gupta, Swati, Bisbe, Anna, Aluri, Krishna, Szeto, John, Kumar, Pawan, LeBlanc, Sarah, Racie, Tim, Brown, Christopher R, Castoreno, Adam, Guenther, Dale C, Jadhav, Vasant, Maier, Martin A, Plavec, Janez, Egli, Martin, Manoharan, Muthiah, Zlatev, Ivan

“…In order to achieve efficient therapeutic post-transcriptional gene-silencing mediated by the RNA interference (RNAi) pathway, small interfering RNAs (siRNAs)…”
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Recognition of double-stranded DNA using LNA-modified toehold Invader probes by Adhikari, Shiva P, Vukelich, Philip, Guenther, Dale C, Karmakar, Saswata, Hrdlicka, Patrick J

“…Development of molecules capable of binding to specific sequences of double-stranded (ds) DNA continues to attract considerable interest, as this may yield…”
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Single-Stranded Hairpin Loop RNAs (loopmeRNAs) Potently Induce Gene Silencing through the RNA Interference Pathway by Aluri, Krishna C., Datta, Dhrubajyoti, Waldron, Scott, Taneja, Nate, Qin, June, Donnelly, Daniel P., Theile, Christopher S., Guenther, Dale C., Lei, Li, Harp, Joel M., Pallan, Pradeep S., Egli, Martin, Zlatev, Ivan, Manoharan, Muthiah

“…Synthetic small interfering RNAs conjugated to trivalent N-acetylgalactosamine (GalNAc) are clinically validated drugs for treatment of liver diseases…”
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4′‑C‑Methoxy-2′-deoxy-2′-fluoro Modified Ribonucleotides Improve Metabolic Stability and Elicit Efficient RNAi-Mediated Gene Silencing by Malek-Adamian, Elise, Guenther, Dale C, Matsuda, Shigeo, Martínez-Montero, Saúl, Zlatev, Ivan, Harp, Joel, Burai Patrascu, Mihai, Foster, Donald J, Fakhoury, Johans, Perkins, Lydia, Moitessier, Nicolas, Manoharan, Rajar M, Taneja, Nate, Bisbe, Anna, Charisse, Klaus, Maier, Martin, Rajeev, Kallanthottathil G, Egli, Martin, Manoharan, Muthiah, Damha, Masad J

“…We designed novel 4′-modified 2′-deoxy-2′-fluorouridine (2′-F U) analogues with the aim to improve nuclease resistance and potency of therapeutic siRNAs by…”
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Structural basis for the synergy of 4′- and 2′-modifications on siRNA nuclease resistance, thermal stability and RNAi activity by Harp, Joel M, Guenther, Dale C, Bisbe, Anna, Perkins, Lydia, Matsuda, Shigeo, Bommineni, Gopal R, Zlatev, Ivan, Foster, Donald J, Taneja, Nate, Charisse, Klaus, Maier, Martin A, Rajeev, Kallanthottathil G, Manoharan, Muthiah, Egli, Martin

“…Abstract Chemical modification is a prerequisite of oligonucleotide therapeutics for improved metabolic stability, uptake and activity, irrespective of their…”
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Chimeric siRNAs with chemically modified pentofuranose and hexopyranose nucleotides: altritol-nucleotide (ANA) containing GalNAc-siRNA conjugates: in vitro and in vivo RNAi activity and resistance to 5'-exonuclease by Kumar, Pawan, Degaonkar, Rohan, Guenther, Dale C, Abramov, Mikhail, Schepers, Guy, Capobianco, Marie, Jiang, Yongfeng, Harp, Joel, Kaittanis, Charalambos, Janas, Maja M, Castoreno, Adam, Zlatev, Ivan, Schlegel, Mark K, Herdewijn, Piet, Egli, Martin, Manoharan, Muthiah

“…In this report, we investigated the hexopyranose chemical modification Altriol Nucleic Acid (ANA) within small interfering RNA (siRNA) duplexes that were…”
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Bulged Invader probes: activated duplexes for mixed-sequence dsDNA recognition with improved thermodynamic and kinetic profiles by Guenther, Dale C, Karmakar, Saswata, Hrdlicka, Patrick J

“…Double-stranded oligonucleotides with +1 interstrand zipper arrangements of intercalator-functionalized nucleotides are energetically activated for recognition…”
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Shorter Is Better: The α‑(l)‑Threofuranosyl Nucleic Acid Modification Improves Stability, Potency, Safety, and Ago2 Binding and Mitigates Off-Target Effects of Small Interfering RNAs by Matsuda, Shigeo, Bala, Saikat, Liao, Jen-Yu, Datta, Dhrubajyoti, Mikami, Atsushi, Woods, Lauren, Harp, Joel M., Gilbert, Jason A., Bisbe, Anna, Manoharan, Rajar M., Kim, MaryBeth, Theile, Christopher S., Guenther, Dale C., Jiang, Yongfeng, Agarwal, Saket, Maganti, Rajanikanth, Schlegel, Mark K., Zlatev, Ivan, Charisse, Klaus, Rajeev, Kallanthottathil G., Castoreno, Adam, Maier, Martin, Janas, Maja M., Egli, Martin, Chaput, John C., Manoharan, Muthiah

“…Chemical modifications are necessary to ensure the metabolic stability and efficacy of oligonucleotide-based therapeutics. Here, we describe analyses of the…”
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Role of a “Magic” Methyl: 2′-Deoxy-2′-α-F-2′-β‑C‑methyl Pyrimidine Nucleotides Modulate RNA Interference Activity through Synergy with 5′-Phosphate Mimics and Mitigation of Off-Target Effects by Guenther, Dale C., Mori, Shohei, Matsuda, Shigeo, Gilbert, Jason A., Willoughby, Jennifer L. S., Hyde, Sarah, Bisbe, Anna, Jiang, Yongfeng, Agarwal, Saket, Madaoui, Mimouna, Janas, Maja M., Charisse, Klaus, Maier, Martin A., Egli, Martin, Manoharan, Muthiah

“…Although 2′-deoxy-2′-α-F-2′-β-C-methyl (2′-F/Me) uridine nucleoside derivatives are a successful class of antiviral drugs, this modification had not been…”
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Impact of non-nucleotidic bulges on recognition of mixed-sequence dsDNA by pyrene-functionalized Invader probes by Guenther, Dale C, Emehiser, Raymond G, Inskeep, Allison, Karmakar, Saswata, Hrdlicka, Patrick J

“…Invader probes, i.e., DNA duplexes modified with +1 interstrand zippers of intercalator-functionalized nucleotides like 2'-O-(pyren-1-yl)methyl-RNA monomers,…”
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Recognition of Mixed-Sequence DNA Duplexes: Design Guidelines for Invaders Based on 2′‑O‑(Pyren-1-yl)methyl-RNA Monomers by Karmakar, Saswata, Guenther, Dale C, Hrdlicka, Patrick J

“…The development of agents that recognize mixed-sequence double-stranded DNA (dsDNA) is desirable because of their potential as tools for detection, regulation,…”
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Recognition of mixed-sequence DNA using double-stranded probes with interstrand zipper arrangements of O2'-triphenylene- and coronene-functionalized RNA monomers by Karmakar, Saswata, Guenther, Dale C, Gibbons, Bradley C, Hrdlicka, Patrick J

“…Development of hybridization-based probes that enable recognition of specific mixed-sequence double-stranded DNA (dsDNA) regions is of considerable interest…”
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Head-to-head comparison of LNA, MP γPNA, INA and Invader probes targeting mixed-sequence double-stranded DNA by Emehiser, Raymond G, Hall, Eric, Guenther, Dale C, Karmakar, Saswata, Hrdlicka, Patrick J

“…Four probe chemistries are characterized and compared with respect to thermal denaturation temperatures (Tms), thermodynamic parameters associated with duplex…”
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Sandwich assay for mixed-sequence recognition of double-stranded DNA: invader-based detection of targets specific to foodborne pathogens by Denn, Benjamin, Karmakar, Saswata, Guenther, Dale C, Hrdlicka, Patrick J

“…A 96-well plate sandwich assay based on Invader capture/signalling probes is used to recognize 28-mer mixed-sequence dsDNA targets specific to Salmonella…”
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Carbohydrate-Functionalized Locked Nucleic Acids: Oligonucleotides with Extraordinary Binding Affinity, Target Specificity, and Enzymatic Stability by Kaura, Mamta, Guenther, Dale C, Hrdlicka, Patrick J

“…Three different C5-carbohydrate-functionalized LNA uridine phosphoramidites were synthesized and incorporated into oligodeoxyribonucleotides…”
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Synthesis and biophysical characterization of oligonucleotides modified with O2'-alkylated RNA monomers featuring substituted pyrene moieties by Karmakar, Saswata, Horrocks, Tyler, Gibbons, Bradley C, Guenther, Dale C, Emehiser, Raymond, Hrdlicka, Patrick J

“…Over the past three decades, a wide range of pyrene-functionalized oligonucleotides have been developed and explored for potential applications in material…”
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Recognition of double-stranded DNA using energetically activated duplexes with interstrand zippers of 1-, 2- or 4-pyrenyl-functionalized O2'-alkylated RNA monomers by Karmakar, Saswata, Madsen, Andreas S, Guenther, Dale C, Gibbons, Bradley C, Hrdlicka, Patrick J

“…Despite advances with triplex-forming oligonucleotides, peptide nucleic acids, polyamides and--more recently--engineered proteins, there remains an urgent need…”
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Invader probes: Harnessing the energy of intercalation to facilitate recognition of chromosomal DNA for diagnostic applications by Guenther, Dale C, Anderson, Grace H, Karmakar, Saswata, Anderson, Brooke A, Didion, Bradley A, Guo, Wei, Verstegen, John P, Hrdlicka, Patrick J

“…Development of probes capable of recognizing specific regions of chromosomal DNA has been a long-standing goal for chemical biologists. Current strategies such…”
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Synthesis and Biophysical Properties of C5-Functionalized LNA (Locked Nucleic Acid) by Kumar, Pawan, Østergaard, Michael E, Baral, Bharat, Anderson, Brooke A, Guenther, Dale C, Kaura, Mamta, Raible, Daniel J, Sharma, Pawan K, Hrdlicka, Patrick J

“…Oligonucleotides modified with conformationally restricted nucleotides such as locked nucleic acid (LNA) monomers are used extensively in molecular biology and…”
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