A confocal setup to measure transmembrane potential in cardiac tissue with very high spatial and temporal resolution

A confocal setup to measure transmembrane potential in cardiac tissue with very high spatial and temporal resolution

We used a fast, fluorescent, potential sensitive indicator (di-4-ANEPPS) in combination with a specially designed setup which consists of an fluorescence microscope, a frequency doubled Nd:YAG laser and a 256 photodiode array system. The setup is especially designed to investigate tissue preparation...

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Bibliographic Details
Published in:Proceedings of the First Joint BMES/EMBS Conference : serving humanity advancing technology, Oct. 13-16, 99, Atlanta, GA, USA Vol. 1; pp. 164 vol.1 - 164
Main Authors: Dapra, D., Muenzer, T., Ahammer, H., Guenter, C.I., Lackner, M., Windisch, H.
Format: Conference Proceeding Journal Article
Language:English
Published: IEEE 1999
Subjects:
Bioelectric potentials
Cell membranes
Fluorescence
Frequency
Image reconstruction
Microscopy
Muscle
Muscles
Neodymium lasers
Optical arrays
Optical design
Optical microscopy
Photodiodes
Signal resolution
Spatial resolution
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Summary:We used a fast, fluorescent, potential sensitive indicator (di-4-ANEPPS) in combination with a specially designed setup which consists of an fluorescence microscope, a frequency doubled Nd:YAG laser and a 256 photodiode array system. The setup is especially designed to investigate tissue preparations in the mm range. The common setup can be reconstructed easily to a confocal laser microscope. This allows confocal microscopic detection of transmembrane potential with high temporal and spatial resolution. The axial resolution of the confocal results in a FWHM better then 15 /spl mu/m. Signals from papillary muscle were compared to images recorded by a CLSM (Leica TCS NT).
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-2
ObjectType-Feature-1
content type line 23
SourceType-Conference Papers & Proceedings-1
ObjectType-Conference-3
ISBN:0780356748
9780780356740
9780780356757
0780356756
ISSN:1094-687X
0589-1019
1558-4615
DOI:10.1109/IEMBS.1999.802209