L-Arginine Modulates Intestinal Inflammation in Rats Submitted to Mesenteric Ischemia-Reperfusion Injury

Abstract Background The goal of this study was to investigate whether exogenous offer of L-arginine (LARG) modulates the gene expression of intestinal dysfunction caused by ischemia and reperfusion. Methods Eighteen Wistar-EPM1 male rats (250–300 g) were anesthetized and subjected to laparotomy. The...

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Published in:	Transplantation proceedings Vol. 48; no. 2; pp. 512 - 515
Main Authors:	Taha, M.O, de Oliveira, J.V, Dias Borges, M, de Lucca Melo, F, Gualtieri, F.G, e Silva Aidar, A.L, Pacheco, R.L, de Melo Alexandre e Silva, T, Klajner, R.K, Iuamoto, L.R, Munhoz Torres, L, Morais Mendes de Paula, B.J, de Campos, K, Souza de Oliveira, I, Fagundes, D.J
Format:	Journal Article
Language:	English
Published:	United States Elsevier Inc 01-03-2016
Subjects:	Animals Apoptosis Arginine - pharmacology bcl-2-Associated X Protein - genetics bcl-2-Associated X Protein - metabolism Intestine, Small - blood supply Intestine, Small - metabolism Intestine, Small - pathology Ischemia - complications Ischemia - metabolism Ischemia - pathology Male Mesenteric Artery, Superior Random Allocation Rats Rats, Wistar Reperfusion Injury - etiology Reperfusion Injury - metabolism Reperfusion Injury - pathology RNA, Messenger - genetics RNA, Messenger - metabolism Surgery
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Summary:	Abstract Background The goal of this study was to investigate whether exogenous offer of L-arginine (LARG) modulates the gene expression of intestinal dysfunction caused by ischemia and reperfusion. Methods Eighteen Wistar-EPM1 male rats (250–300 g) were anesthetized and subjected to laparotomy. The superior mesenteric vessels were exposed, and the rats were randomized into 3 groups (n = 6): the control group (CG), with no superior mesenteric artery interruption; the ischemia/reperfusion group (IRG), with 60 minutes of ischemia and 120 minutes of reperfusion and saline injections; and the L-arginine group (IRG + LARG), with L-arginine injected in the femoral vein 5 minutes before ischemia, 5 minutes after reperfusion, and after 55 minutes of reperfusion. The total RNA was extracted and purified from samples of the small intestine. The concentration of each total RNA sample was determined by using spectrophotometry. The first-strand complementary DNA (cDNA) was synthesized in equal amounts of cDNA and the Master Mix SYBR Green qPCR Mastermix (SABiosciences, a Qiagen Company, Frederick, Md). Amounts of cDNA and Master Mix SYBR Green qPCR Mastermix were distributed to each well of the polymerase chain reaction microarray plate containing the predispensed gene-specific primer sets for Bax and Bcl2. Each sample was evaluated in triplicate, and the Student t test was applied to validate the homogeneity of each gene expression reaction ( P < .05). Results The gene expression of Bax in IRG (+1.48) was significantly higher than in IRG-LARG (+9.69); the expression of Bcl2L1 in IRG (+1.01) was significantly higher than IRG-LARG (+22.89). Conclusions The apoptotic cell pathway of 2 protagonists showed that LARG improves the gene expression of anti-apoptotic Bcl2l1 (Bcl2-like 1) more than the pro-apoptotic Bax (Bcl2-associated X protein).
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0041-1345 1873-2623
DOI:	10.1016/j.transproceed.2015.12.063