Search Results - "Guérin, S L"

A short protocol for micro-purification of nuclear proteins from whole animal tissue by Roy, R J, Gosselin, P, Guérin, S L

“…Although a number of small-scale procedures have been described for the preparation of crude nuclear extracts from established cell lines, none were provided…”
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Transcription of the mouse secretory protease inhibitor p12 gene is activated by the developmentally regulated positive transcription factor Sp1 by ROBIDOUX, S, GOSSELIN, P, HARVEY, M, LECLERC, S, GUERIN, S. L

“…Article Usage Stats Services MCB Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley…”
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An improved CAT assay for promoter analysis in either transgenic mice or tissue culture cells by Pothier, F, Ouellet, M, Julien, J P, Guérin, S L

“…We have developed an improved method for determining CAT activity directed by stably (transgenic mice) or transiently (tissue culture cell lines) introduced…”
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Binding of a nuclear protein to the rat growth hormone silencer element by Roy, René J., Gosselin, Pierre, Anzivino, Matthew J., Moore, David D., Guérin, Sylvain L.

“…The rat growth hormone (rGH) gene is uniquely expressed in a subset of cells from the anterior pituitary. This strongly cell type specific expression is…”
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Members of the Nuclear Factor 1 Family Reduce the Transcriptional Potential of the Nuclear Receptor LXRα Promoter by Steffensen, Knut R., Holter, Elin, Tobin, Kari A.R., Leclerc, Steeve, Gustafsson, Jan-Åke, Guérin, Sylvain L., Eskild, Winnie

“…Expression of the LXRα nuclear receptor in liver is predicted to affect cholesterol and lipid metabolism. Here we show that a short fragment from the LXRα gene…”
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Electrophoretic mobility shift assays for the analysis of DNA-protein interactions by Laniel, M A, Béliveau, A, Guérin, S L

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Two Mechanisms for the Extinction of Gene Expression in Hybrid Cells by Tripputi, Pasquale, Guérin, Sylvain L., Moore, David D.

“…When two different mammalian cell types are fused to generate a stable hybrid cell line, genes that are active in only one of the parents are frequently shut…”
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Multiple nuclear regulatory proteins bind a single cis-acting promoter element to control basal transcription of the human alpha 4 integrin gene in corneal epithelial cells by Larouche, K, Leclerc, S, Giasson, M, Guérin, S L

“…Expression of the fibronectin-binding integrin alpha 4 beta 1 has been postulated to be an important event in the process of corneal epithelial wound healing…”
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Overlapping cis-acting elements located in the first intron of the gene for type I 3 beta-hydroxysteroid dehydrogenase modulate its transcriptional activity by Guérin, S L, Leclerc, S, Verreault, H, Labrie, F, Luu-The, V

“…High levels of expression for the gene encoding human type I 3 beta-hydroxysteroid dehydrogenase (3 beta-HSDI) have been detected in placenta and skin but not…”
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Multiple cloning sites from mammalian expression vectors interfere with gene promoter studies in vitro by Béliveau, A., Leclerc, S., Rouleau, M., Guérin, S. L.

“…When performing transcriptional analyses, reporter gene‐expression vectors are used to insert promoter fragments through the selected use of a multiple cloning…”
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Identification of a DNA-binding site for the transcription factor GC2 in the promoter region of the p12 gene and repression of its positive activity by upstream negative regulatory elements by Guérin, S L, Pothier, F, Robidoux, S, Gosselin, P, Parker, M G

“…Mouse secretory protease inhibitor p12 is significantly transcribed by the cells from the seminal vesicle, the coagulating gland, the ventral prostate, and to…”
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The rat growth hormone and human cellular retinol binding protein 1 genes share homologous NF1-like binding sites that exert either positive or negative influences on gene expression in vitro by Leclerc, S, Eskild, W, Guérin, S L

“…High levels of expression for the rat growth hormone (rGH) gene are restricted to the somatotroph cells of the anterior pituitary. Previously, we have shown…”
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Improving sensitivity of the electrophoretic mobility shift assay by restricting tissue phosphatase activities by Laniel, M A, Guérin, S L

“…Elucidating DNA-protein interactions at the molecular level is a prerequisite in understanding the way a transcriptionally active gene is regulated in various…”
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Optimization of competitor poly(dI-dC).poly(dI-dC) levels is advised in DNA-protein interaction studies involving enriched nuclear proteins by Larouche, K, Bergeron, M J, Leclerc, S, Guérin, S L

“…Procedures used for investigating DNA-protein interactions, such as the electrophoretic mobility shift assay (EMSA) or DNasel footprinting, require that…”
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A simple apparatus for fast and inexpensive recovery of DNA from polyacrylamide gels by Harvey, M, Brisson, I, Guérin, S L

“…A typical elution procedure for DNA should be easy, quick, inexpensive, highly efficient and must provide DNA of good purity and quality to carry out any…”
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Can we produce a human corneal equivalent by tissue engineering? by Germain, Lucie, Carrier, Patrick, Auger, François A, Salesse, Christian, Guérin, Sylvain L

“…Tissue engineering is progressing rapidly. Bioengineered substitutes are already available for experimental applications and some clinical purposes such as…”
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The 30‐kDa rat liver transcription factor nuclear factor 1 binds the rat growth‐hormone proximal silencer by ROY, René J., GUÉRIN, Sylvain L.

“…Transcription of the gene encoding rat growth hormone is under the influence of cis‐acting negative regulatory elements termed silencers. We showed previously…”
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Salt-dependent formation of DNA/protein complexes in vitro, as viewed by the gel mobility shift assay by Robidoux, S, Guérin, S L, Eskild, W, Kroepelin, C F, Hansson, V

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Reconstructed human cornea produced in vitro by tissue engineering by Germain, L, Auger, F A, Grandbois, E, Guignard, R, Giasson, M, Boisjoly, H, Guérin, S L

“…The aim of the present study was to produce a reconstructed human cornea in vitro by tissue engineering and to characterize the expression of integrins and…”
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The US‐1 element from the gene encoding rat poly (ADP‐ribose) polymerase binds the transcription factor Sp1 by POTVIN, Frédeéric, ROY, René J., POIRIER, Guy G., GUÉRIN, Sylvain L.

“…By comparing the upstream DNA sequence of the rat and human genes encoding poly(ADP‐ribose) polymerase (PARP), we have defined a 16‐bp conserved region and…”
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