Search Results - "Gruys, K J"

Metabolic engineering of Arabidopsis and Brassica for poly(3-hydroxybutyrate-co-3-hydroxyvalerate) copolymer production by Slater, S, Mitsky, T.A, Houmiel, K.L, Hao, M, Reiser, S.E, Taylor, N.B, Tran, M, Valentin, H.E, Rodriguez, D.J, Stone, D.A

“…Poly(hydroxyalkanoates) are natural polymers with thermoplastic properties. One polymer of this class with commercial applicability,…”
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Characterization and cloning of an (R)-specific trans-2,3-enoylacyl-CoA hydratase from Rhodospirillum rubrum and use of this enzyme for PHA production in Escherichia coli by REISER, S. E, MITSKY, T. A, GRUYS, K. J

“…An (R)-trans-2,3-enoylacyl-CoA hydratase was purified to near-homogeneity from Rhodospirillum rubrum. Protein sequencing of enriched protein fractions allowed…”
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Polyhydroxyalkanoate accumulation in Burkholderia sp.: a molecular approach to elucidate the genes involved in the formation of two homopolymers consisting of short-chain-length 3-hydroxyalkanoic acids by DE ANDRADE RODRIGUES, M. F, VALENTIN, H. E, BERGER, P. A, TRAN, M, ASRAR, J, GRUYS, K. J, STEINBÜCHEL, A

“…Burkholderia sp. accumulates polyhydrox-yalkanoates (PHAs) containing 3-hydroxybutyrate and 3-hydroxy-4-pentenoic acid when grown on mineral media under…”
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Application of a Propionyl Coenzyme A Synthetase for Poly(3-Hydroxypropionate-co-3-Hydroxybutyrate) Accumulation in Recombinant Escherichia coli by VALENTIN, Henry E, MITSKY, Timothy A, MAHADEO, Debbie A, TRAN, Minhtien, GRUYS, Kenneth J

“…Classifications Services AEM Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit…”
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Poly(3-hydroxybutyrate-co-4-hydroxybutyrate) formation from γ-aminobutyrate and glutamate by Valentin, Henry E., Reiser, Steven, Gruys, Kenneth J.

“…To provide 4‐hydroxybutyryl‐CoA for poly(3‐hydroxybutyrate‐co‐4‐hydroxybutyrate) formation from glutamate in Escherichia coli, an acetyl‐CoA:4‐hydroxybutyrate…”
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Electron Microscopy of Cytochrome c Oxidase Crystals: Labeling of Subunit III with a Monomaleimide Undecagold Cluster Compound by Crum, J, Gruys, K. J, Frey, T. G

“…Two-dimensional crystals of beef heart mitochondrial cytochrome c oxidase dimers were labeled at Cys-115 of subunit III with a monomaleimide derivative of an…”
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Substrate synergism and the steady-state kinetic reaction mechanism for EPSP synthase from Escherichia coli by Gruys, Kenneth J, Walker, Mark C, Sikorski, James A

“…Previous studies of Escherichia coli 5-enolpyruvoylshikimate-3-phosphate synthase (EPSPS, EC 2.5.1.19) have suggested that the kinetic reaction mechanism for…”
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Multiple β-Ketothiolases Mediate Poly(β-Hydroxyalkanoate) Copolymer Synthesis in Ralstonia eutropha by Slater, S, Houmiel, K L, Tran, M, Mitsky, T A, Taylor, N B, Padgette, S R, Gruys, K J

“…Article Usage Stats Services JB Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley…”
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Steady-state kinetic evaluation of the reverse reaction for Escherichia coli 5-enolpyruvoylshikimate-3-phosphate synthase by Gruys, K J, Marzabadi, M R, Pansegrau, P D, Sikorski, J A

“…Recently it has been found that the kinetic mechanism for Escherichia coli 5-enolpyruvoylshikimate-3-phosphate synthase (EPSPS) in the forward direction is…”
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Metal interactions with beef heart mitochondrial ATPase by Daggett, S G, Gruys, K J, Schuster, S M

“…Atomic absorption and electron paramagnetic resonance spectroscopy were used to study the metal binding sites of beef heart mitochondrial ATPase (F1)…”
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alpha-Ketoglutarate dehydrogenase complex of Escherichia coli. A hybrid complex containing pyruvate dehydrogenase subunits from pyruvate dehydrogenase complex by Steginsky, C A, Gruys, K J, Frey, P A

“…The alpha-ketoglutarate dehydrogenase complex of Escherichia coli utilizes pyruvate as a poor substrate, with an activity of 0.082 units/mg of protein compared…”
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Metal-nucleotide structural characteristics during catalysis by beef heart mitochondrial F1 by Gruys, K J, Urbauer, J L, Schuster, S M

“…This study examined the nature of the metal-nucleotide complexes which serve as substrates, products, and intermediates in the beef heart mitochondrial ATPase…”
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2-Acetylthiamin pyrophosphate (acetyl-TPP) pH-rate profile for hydrolysis of acetyl-TPP and isolation of acetyl-TPP as a transient species in pyruvate dehydrogenase catalyzed reactions by Gruys, Kenneth J, Datta, Asit, Frey, Perry A

“…Rate constants for the hydrolysis of acetyl-TPP were measured between pH values of 2.5 and 7.5 and plotted as log kobs versus pH. The pH-rate profile defined…”
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Synthesis and properties of 2-acetylthiamin pyrophosphate: an enzymatic reaction intermediate by Gruys, Kenneth J, Halkides, Christopher J, Frey, Perry A

“…The synthesis of 2-acetylthiamin pyrophosphate (acetyl-TPP) is described. The synthesis of this compound is accomplished at 23 degrees C by the oxidation of…”
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Biosynthesis and Characterization of Poly(3-hydroxy-4-pentenoic acid) by Valentin, Henry E, Berger, Pierre A, Gruys, Kenneth J, Filomena de Andrade Rodrigues, Maria, Steinbüchel, Alexander, Tran, Minhtien, Asrar, Jawed

“…Burkholderia sp. was grown on sucrose-containing mineral salts medium with phosphate limitation to induce poly(hydroxyalkanoate) (PHA) accumulation. Under…”
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PHA production, from bacteria to plants by Valentin, Henry E., Broyles, Debra L., Casagrande, Laura A., Colburn, Susan M., Creely, Wendi L., DeLaquil, Pamela A., Felton, Heather M., Gonzalez, Kathleen A., Houmiel, Kathryn L., Lutke, Kevin, Mahadeo, Debbie A., Mitsky, Timothy A., Padgette, Stephen R., Reiser, Steven E., Slater, Steven, Stark, David M., Stock, Robert T., Stone, Deborah A., Taylor, Nancy B., Thorne, Gregory M., Tran, Minhtien, Gruys, Kenneth J.

“…The genes encoding the polyhydroxyalkanoate (PHA) biosynthetic pathway in Ralstonia eutropha (3-ketothiolase, phaA or bktB; acetoacetyl-CoA reductase, phaB;…”
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Poly(beta-hydroxybutyrate) production in oilseed leukoplasts of Brassica napus by Houmiel, K.L, Slater, S, Broyles, D, Casagrande, L, Colburn, S, Gonzalez, K, Mitsky, T.A, Reiser, S.E, Shah, D, Taylor, N.B

“…Polyhydroxyalkanoates (PHAs) comprise a class of biodegradable polymers which offer an environmentally sustainable alternative to petroleum-based plastics…”
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An EPSP Synthase Inhibitor Joining Shikimate 3-Phosphate with Glyphosate:  Synthesis and Ligand Binding Studies by Marzabadi, Mohammad R., Gruys, Kenneth J., Pansegrau, Paul D., Walker, Mark C., Yuen, Henry K., Sikorski, James A.

“…A novel EPSP synthase inhibitor 4 has been designed and synthesized to probe the configurational details of glyphosate recognition in its herbicidal ternary…”
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Synthesis of gamma-monodentate and beta, gamma-bidentate CrITP. Isolation and characterization of the two chelation isomers and the individual diastereomers of beta, gamma-bidentate CrITP by Gruys, K J, Schuster, S M

“…The two chelation isomers of CrITP, gamma-monodentate and beta, gamma-bidentate CrITP, as well as the diastereomers of beta, gamma-bidentate CrITP were…”
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Arabidopsis vitamin E pathway gene5-1 Mutant Reveals a Critical Role for Phytol Kinase in Seed Tocopherol Biosynthesis by Valentin, Henry E, Lincoln, Kim, Moshiri, Farhad, Jensen, Pamela K, Qi, Qungang, Venkatesh, Tyamagondlu V, Karunanandaa, Balasulojini, Baszis, Susan R, Norris, Susan R, Savidge, Beth, Gruys, Kenneth J, Last, Robert L

“…We report the identification and characterization of a low tocopherol Arabidopsis thaliana mutant, vitamin E pathway gene5-1 (vte5-1), with seed tocopherol…”
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