The SPL transcription factor genes are potential targets for epigenetic regulation in response to drought stress in chickpea (C. arietinum L.)

The SPL transcription factor genes are potential targets for epigenetic regulation in response to drought stress in chickpea (C. arietinum L.)

Background Crop improvement for tolerance to various biotic and abiotic stress factors necessitates understanding the key gene regulatory mechanisms. One such mechanism of gene regulation involves changes in cytosine methylation at the gene body and flanking regulatory sequences. The present study w...

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Bibliographic Details
Published in:Molecular biology reports Vol. 50; no. 6; pp. 5509 - 5517
Main Authors: Yadav, Sheel, Yadava, Yashwant K., Meena, Shashi, Singh, Lalbahadur, Kansal, Rekha, Grover, Monender, M.S., Nimmy, Bharadwaj, C., Paul, Vijay, Gaikwad, Kishor, Jain, Pradeep K.
Format: Journal Article
Language:English
Published: Dordrecht Springer Netherlands 01-06-2023
Springer Nature B.V
Subjects:
Animal Anatomy
Animal Biochemistry
Biomedical and Life Sciences
Bisulfite
Cicer - genetics
Cicer - metabolism
Crop improvement
Cytosine
DNA methylation
Drought resistance
Droughts
Epigenesis, Genetic
Epigenetics
Gene Expression Regulation, Plant - genetics
Gene regulation
Genes
Genotypes
Histology
Life Sciences
Morphology
Next-generation sequencing
Polymerase chain reaction
Regulatory sequences
Short Communication
Stress, Physiological - genetics
Transcription factors
Transcription Factors - genetics
Transcription Factors - metabolism
DNA methylation
Epialleles
Chickpea
Transcription factors
Drought
SPL
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Summary:Background Crop improvement for tolerance to various biotic and abiotic stress factors necessitates understanding the key gene regulatory mechanisms. One such mechanism of gene regulation involves changes in cytosine methylation at the gene body and flanking regulatory sequences. The present study was undertaken to identify genes which might be potential targets of drought-induced DNA methylation in chickpea. Methods and results Two chickpea genotypes, which contrast for drought tolerance, were subjected to drought stress conditions and their differential response was studied by analysing different morpho-physiological traits. Utilizing the in-house, high throughput sequencing data, the SQUAMOSA promoter-binding (SBP) protein-like ( SPL ) transcription factor genes were identified to be differentially methylated and expressed amongst the two genotypes, in response to drought stress. The methylation status of one of these genes was examined and validated through bisulfite PCR (BS-PCR). The identified genes could be possible homologs to known epialleles and can therefore serve as potential epialleles which can be utilized for crop improvement in chickpea. Conclusion The SPL TF genes are potential targets of epigenetic regulation in response to drought stress in chickpea. Since these are TFs, they might play important roles in controlling the expression of other genes, thus contributing to differential drought response of the two genotypes.
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ISSN:0301-4851
1573-4978
DOI:10.1007/s11033-023-08347-y