Search Results - "Grose, Carissa"

Farnesylated and methylated KRAS4b: high yield production of protein suitable for biophysical studies of prenylated protein-lipid interactions by Gillette, William K., Esposito, Dominic, Abreu Blanco, Maria, Alexander, Patrick, Bindu, Lakshman, Bittner, Cammi, Chertov, Oleg, Frank, Peter H., Grose, Carissa, Jones, Jane E., Meng, Zhaojing, Perkins, Shelley, Van, Que, Ghirlando, Rodolfo, Fivash, Matthew, Nissley, Dwight V., McCormick, Frank, Holderfield, Matthew, Stephen, Andrew G.

“…Prenylated proteins play key roles in several human diseases including cancer, atherosclerosis and Alzheimer’s disease. KRAS4b, which is frequently mutated in…”
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Chemical Control of a CRISPR-Cas9 Acetyltransferase by Shrimp, Jonathan H, Grose, Carissa, Widmeyer, Stephanie R. T, Thorpe, Abigail L, Jadhav, Ajit, Meier, Jordan L

“…Lysine acetyltransferases (KATs) play a critical role in the regulation of transcription and other genomic functions. However, a persistent challenge is the…”
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Producing recombinant proteins in Vibrio natriegens by Smith, Matthew, Hernández, José Sánchez, Messing, Simon, Ramakrishnan, Nitya, Higgins, Brianna, Mehalko, Jennifer, Perkins, Shelley, Wall, Vanessa E, Grose, Carissa, Frank, Peter H, Cregger, Julia, Le, Phuong Vi, Johnson, Adam, Sherekar, Mukul, Pagonis, Morgan, Drew, Matt, Hong, Min, Widmeyer, Stephanie R T, Denson, John-Paul, Snead, Kelly, Poon, Ivy, Waybright, Timothy, Champagne, Allison, Esposito, Dominic, Jones, Jane, Taylor, Troy, Gillette, William

“…The diversity of chemical and structural attributes of proteins makes it inherently difficult to produce a wide range of proteins in a single recombinant…”
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Relocation of the attTn7 Transgene Insertion Site in Bacmid DNA Enhances Baculovirus Genome Stability and Recombinant Protein Expression in Insect Cells by Pijlman, Gorben P, Grose, Carissa, Hick, Tessy A H, Breukink, Herman E, van den Braak, Robin, Abbo, Sandra R, Geertsema, Corinne, van Oers, Monique M, Martens, Dirk E, Esposito, Dominic

“…Baculovirus expression vectors are successfully used for the commercial production of complex (glyco)proteins in eukaryotic cells. The genome engineering of…”
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Structural dynamics of RAF1-HSP90-CDC37 and HSP90 complexes reveal asymmetric client interactions and key structural elements by Finci, Lorenzo I., Chakrabarti, Mayukh, Gulten, Gulcin, Finney, Joseph, Grose, Carissa, Fox, Tara, Yang, Renbin, Nissley, Dwight V., McCormick, Frank, Esposito, Dominic, Balius, Trent E., Simanshu, Dhirendra K.

“…RAF kinases are integral to the RAS-MAPK signaling pathway, and proper RAF1 folding relies on its interaction with the chaperone HSP90 and the cochaperone…”
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A review of alternative promoters for optimal recombinant protein expression in baculovirus-infected insect cells by Grose, Carissa, Putman, Zoe, Esposito, Dominic

“…Generating recombinant proteins in insect cells has been made possible via the use of the Baculovirus Expression Vector System (BEVS). Despite the success of…”
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High quality protein microarray using in situ protein purification by Kwon, Keehwan, Grose, Carissa, Pieper, Rembert, Pandya, Gagan A, Fleischmann, Robert D, Peterson, Scott N

“…In the postgenomic era, high throughput protein expression and protein microarray technologies have progressed markedly permitting screening of therapeutic…”
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Production of authentic geranylgeranylated KRAS4b using an engineered baculovirus system by Procter, Lauren, Grose, Carissa, Esposito, Dominic

“…Protein prenylation is a vital eukaryotic post-translational modification which permits interaction of proteins with cellular membranes. Prenylated proteins…”
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Production of Farnesylated and Methylated Proteins in an Engineered Insect Cell System by Gillette, William, Frank, Peter, Perkins, Shelley, Drew, Matthew, Grose, Carissa, Esposito, Dominic

“…Protein prenylation is a common posttranslational modification that enhances the ability of proteins to interact with membrane components within the cell. In…”
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Improved production of class I phosphatidylinositol 4,5-bisphosphate 3-kinase by Messing, Simon, Widmeyer, Stephanie R.T., Denson, John-Paul, Mehalko, Jennifer, Wall, Vanessa E., Drew, Matthew, Snead, Kelly, Hong, Min, Grose, Carissa, Esposito, Dominic, Gillette, William

“…Phosphatidylinositol 4,5-bisphosphate 3-kinases (PI3K) are a family of kinases whose activity affects pathways needed for basic cell functions. As a result,…”
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Chemoproteomics Yields a Selective Molecular Host for Acetyl-CoA by Lieberman, Whitney K., Brown, Zachary A., Kantner, Daniel S., Jing, Yihang, Megill, Emily, Evans, Nya D., Crawford, McKenna C., Jhulki, Isita, Grose, Carissa, Jones, Jane E., Snyder, Nathaniel W., Meier, Jordan L.

“…Chemoproteomic profiling is a powerful approach to define the selectivity of small molecules and endogenous metabolites with the human proteome. In addition to…”
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Abstract 3925: Production and biochemical characterization of farnesylated and methylated KRAS4b using engineered baculovirus by Stephen, Andrew G., Gillette, William K., Grose, Carissa, Esposito, Dominic

“…Abstract KRAS4b, which is frequently mutated in pancreatic, colon and lung cancers, is processed in several steps including farnesylation by…”
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Abstract 1872: Development of reference reagents to accelerate research on the RAS pathway by Esposito, Dominic, Mehalko, Jennifer, Wall, Vanessa, Grose, Carissa, Gillette, William, Stephens, Robert

“…Abstract The RAS Reference Reagents program within the NCI RAS Initiative is produces and distributes qualified DNA vectors, cell lines, and viruses to the…”
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Simultaneous non-contiguous deletions using large synthetic DNA and site-specific recombinases by Krishnakumar, Radha, Grose, Carissa, Haft, Daniel H, Zaveri, Jayshree, Alperovich, Nina, Gibson, Daniel G, Merryman, Chuck, Glass, John I

“…Toward achieving rapid and large scale genome modification directly in a target organism, we have developed a new genome engineering strategy that uses a…”
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Genome Assembly and Annotation of the Trichoplusia ni Tni-FNL Insect Cell Line Enabled by Long-Read Technologies by Talsania, Keyur, Mehta, Monika, Raley, Castle, Kriga, Yuliya, Gowda, Sujatha, Grose, Carissa, Drew, Matthew, Roberts, Veronica, Cheng, Kwong Tai, Burkett, Sandra, Oeser, Steffen, Stephens, Robert, Soppet, Daniel, Chen, Xiongfeng, Kumar, Parimal, German, Oksana, Smirnova, Tatyana, Hautman, Christopher, Shetty, Jyoti, Tran, Bao, Zhao, Yongmei, Esposito, Dominic

“…derived cell lines are commonly used to enable recombinant protein expression via baculovirus infection to generate materials approved for clinical use and in…”
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Recombinant expression and functional analysis of proteases from Streptococcus pneumoniae, Bacillus anthracis, and Yersinia pestis by Kwon, Keehwan, Hasseman, Jeremy, Latham, Saeeda, Grose, Carissa, Do, Yu, Fleischmann, Robert D, Pieper, Rembert, Peterson, Scott N

“…Uncharacterized proteases naturally expressed by bacterial pathogens represents important topic in infectious disease research, because these enzymes may have…”
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High quality protein microarray using in situprotein purification by Kwon, Keehwan, Grose, Carissa, Pieper, Rembert, Pandya, Gagan A, Fleischmann, Robert D, Peterson, Scott N

“…Abstract Background In the postgenomic era, high throughput protein expression and protein microarray technologies have progressed markedly permitting…”
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A correlation analysis of protein characteristics associated with genome-wide high throughput expression and solubility of Streptococcus pneumoniae proteins by Kwon, Keehwan, Pieper, Rembert, Shallom, Shamira, Grose, Carissa, Kwon, Erika, Do, Yu, Latham, Saeeda, Alami, Hamid, Huang, Shih-Ting, Gatlin, Christine, Papazisi, Leka, Fleischmann, Robert, Peterson, Scott

“…We have developed and evaluated a highly parallel protein expression and purification system using ORFs derived from the pathogenic bacterium Streptococcus…”
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