Development of Cassava ( Manihot esculenta Crantz.) somatic embryos during culture with abscisic acid and activated charcoal

Secondary cassava somatic embryos were cultured on a sequence of developmental media containing either activated charcoal or abscisic acid. Medium supplementation with activated charcoal had a positive effect on both differentiation and subsequent germination. However, sustained exposure to activate...

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Bibliographic Details
Published in:	Journal of plant physiology Vol. 159; no. 4; pp. 437 - 443
Main Authors:	Groll, Jonathan, Gray, Vincent Myles, Mycock, David John
Format:	Journal Article
Language:	English
Published:	Jena Elsevier GmbH 2002 Elsevier Elsevier Science Ltd
Subjects:	Agronomy. Soil science and plant productions Biological and medical sciences Cassava desiccation Economic plant physiology Fundamental and applied biological sciences. Psychology In vitro culture Manihot esculenta maturation Plant physiology and development somatic embryogenesis Tissue cultures, protoplasts desiccation maturation Manihot esculenta somatic embryogenesis Cassava Embryonic development Tissue culture Micropropagation Growth regulator treatment Somatic embryo Dicotyledones Angiospermae Abscisic acid Euphorbiaceae Plant growth substance Spermatophyta Tuber plant
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Summary:	Secondary cassava somatic embryos were cultured on a sequence of developmental media containing either activated charcoal or abscisic acid. Medium supplementation with activated charcoal had a positive effect on both differentiation and subsequent germination. However, sustained exposure to activated charcoal supplemented media beyond the seventh day of differentiation did not appear to be beneficial. Somatic embryos were desiccated over a saturated salt solution of K 2SO 4 for 10 days and allowed to develop into plantlets on a MS medium containing 0.4 μmol/L BAP. Embryo germination was significantly affected by the culture medium used prior to the desiccation treatment, with culture on a medium supplemented with ABA at a level of 0.1 μmol/L prior to desiccation significantly enhancing germination. The number of normal germinants produced as judged by morphological criteria were not however, dependant on ABA supplementation of the culture medium prior to desiccation. Culture on a separate medium for the differentiation of embryos, different to the medium used for the maturation of somatic embryos is indicated.
ISSN:	0176-1617 1618-1328
DOI:	10.1078/0176-1617-00582