Identification of Giant Isoforms of Obscurin in Rat Striated Muscles Using Polyclonal Antibodies

Using produced polyclonal antibodies specific to the N-terminal sequence (residues 61-298) of rat obscurin, we investigated the isoform composition of this protein in 4 striated muscles: myocardium of the left ventricle, diaphragm, skeletal m. gastrocnemius (containing mainly fast fibers), and m. so...

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Bibliographic Details
Published in:	Bulletin of experimental biology and medicine Vol. 177; no. 6; pp. 731 - 735
Main Authors:	Gritsyna, Y. V., Zhalimov, V. K., Uryupina, T. A., Ulanova, A. D., Bobylev, A. G., Vikhlyantsev, I. M.
Format:	Journal Article
Language:	English
Published:	New York Springer US 01-10-2024 Springer Nature B.V
Subjects:	Amino acid sequence Biomedical and Life Sciences Biomedicine Biophysics and Biochemistry Cell Biology Diaphragm Fibers Heart Internal Medicine Isoforms Laboratory Medicine Molecular weight Muscles Myocardium Pathology Polyclonal antibodies Protein composition Skeletal muscle striated muscles; giant muscle proteins; obscurin; obscurin gene (OBSCN)
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Summary:	Using produced polyclonal antibodies specific to the N-terminal sequence (residues 61-298) of rat obscurin, we investigated the isoform composition of this protein in 4 striated muscles: myocardium of the left ventricle, diaphragm, skeletal m. gastrocnemius (containing mainly fast fibers), and m. soleus (containing mainly slow fibers). The m. gastrocnemius , m. soleus , and diaphragm were found to have 2 giant isoforms of obscurin: a smaller A-isoform and a larger B-isoform. Their molecular weights were ~870 and ~1150 kDa in the diaphragm and m. gastrocnemius and ~880 and ~1130 kDa in m. soleus , respectively. The B-isoform to A-isoform ratio was 1:3 in the diaphragm and m. soleus and 1:4 in the m. gastrocnemius . In the left-ventricular myocardium, A-isoform of obscurin with a molecular weight of ~880 kDa was found. No other obscurin isoforms or their fragments within the molecular weight range of 10 up to ~800 kDa were revealed in the investigated rat striated muscles. The antibodies produced are recommended for research into qualitative and quantitative changes of giant obscurin isoforms in rat striated muscles in the norm and during the development of pathological processes.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0007-4888 1573-8221 1573-8221
DOI:	10.1007/s10517-024-06259-w