Search Results - "Grindley, Nigel D. F."

Prechemistry Nucleotide Selection Checkpoints in the Reaction Pathway of DNA Polymerase I and Roles of Glu710 and Tyr766 by Bermek, Oya, Grindley, Nigel D. F, Joyce, Catherine M

“…The accuracy of high-fidelity DNA polymerases such as DNA polymerase I (Klenow fragment) is governed by conformational changes early in the reaction pathway…”
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Fingers-Closing and Other Rapid Conformational Changes in DNA Polymerase I (Klenow Fragment) and Their Role in Nucleotide Selectivity by Joyce, Catherine M, Potapova, Olga, DeLucia, Angela M, Huang, Xuanwei, Basu, Vandana Purohit, Grindley, Nigel D. F

“…We have developed a FRET-based assay for the fingers-closing conformational transition that occurs when a binary complex of DNA polymerase I (Klenow fragment)…”
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Single-molecule analysis reveals the molecular bearing mechanism of DNA strand exchange by a serine recombinase by Bai, Hua, Sun, Mingxuan, Ghosh, Pallavi, Hatfull, Graham F, Grindley, Nigel D.F, Marko, John F

“…Structural and topological data suggest that serine site-specific DNA recombinases exchange duplex DNAs by rigid-body relative rotation of the two halves of…”
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Remote control of DNA-acting enzymes by varying the Brownian dynamics of a distant DNA end by Bai, Hua, Kath, James E., Zörgiebel, Felix Manuel, Sun, Mingxuan, Ghosh, Pallavi, Hatfull, Graham F., Grindley, Nigel D. F., Marko, John F.

“…Enzyme rates are usually considered to be dependent on local properties of the molecules involved in reactions. However, for large molecules, distant…”
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Conformational transitions in DNA polymerase I revealed by single-molecule FRET by Santoso, Yusdi, Joyce, Catherine M, Potapova, Olga, Le Reste, Ludovic, Hohlbein, Johannes, Torella, Joseph P, Grindley, Nigel D.F, Kapanidis, Achillefs N

“…The remarkable fidelity of most DNA polymerases depends on a series of early steps in the reaction pathway which allow the selection of the correct nucleotide…”
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Mechanisms of site-specific recombination by Grindley, Nigel D F, Whiteson, Katrine L, Rice, Phoebe A

“…Integration, excision, and inversion of defined DNA segments commonly occur through site-specific recombination, a process of DNA breakage and reunion that…”
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The Properties of Steric Gate Mutants Reveal Different Constraints within the Active Sites of Y-family and A-family DNA Polymerases by DeLucia, Angela M., Chaudhuri, Santanov, Potapova, Olga, Grindley, Nigel D.F., Joyce, Catherine M.

“…Y-family (lesion-bypass) DNA polymerases show the same overall structural features seen in other members of the polymerase superfamily, yet their active sites…”
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Use of 2-Aminopurine Fluorescence To Examine Conformational Changes during Nucleotide Incorporation by DNA Polymerase I (Klenow Fragment) by Purohit, Vandana, Grindley, Nigel D. F, Joyce, Catherine M

“…We have investigated conformational transitions in the Klenow fragment polymerase reaction by stopped-flow fluorescence using DNA substrates containing the…”
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Conformational Changes during Normal and Error-Prone Incorporation of Nucleotides by a Y-Family DNA Polymerase Detected by 2-Aminopurine Fluorescence by DeLucia, Angela M, Grindley, Nigel D. F, Joyce, Catherine M

“…Y-family polymerases are specialized to carry out DNA synthesis past sites of DNA damage. Their active sites make fewer contacts to their substrates,…”
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The Left End of IS2: a Compromise between Transpositional Activity and an Essential Promoter Function That Regulates the Transposition Pathway by LEWIS, Leslie A, CYLIN, Edruge, HO KYUNG LEE, SABY, Robert, WONG, Wilson, GRINDLEY, Nigel D. F

“…Article Usage Stats Services JB Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley…”
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A Single Side Chain Prevents Escherichia coli DNA Polymerase I (Klenow Fragment) from Incorporating Ribonucleotides by Astatke, Mekbib, Ng, Kimmie, Nigel D. F. Grindley, Joyce, Catherine M.

“…Although nucleic acid polymerases from different families show striking similarities in structure, they maintain stringent specificity for the sugar structure…”
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Distinct Roles of the Active-site Mg2+ Ligands, Asp882 and Asp705, of DNA Polymerase I (Klenow Fragment) during the Prechemistry Conformational Transitions by Bermek, Oya, Grindley, Nigel D.F., Joyce, Catherine M.

“…DNA polymerases catalyze the incorporation of deoxynucleoside triphosphates into a growing DNA chain using a pair of Mg2+ ions, coordinated at the active site…”
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DNA Polymerase Catalysis in the Absence of Watson−Crick Hydrogen Bonds:  Analysis by Single-Turnover Kinetics by Potapova, Olga, Chan, Chikio, DeLucia, Angela M, Helquist, Sandra A, Kool, Eric T, Grindley, Nigel D. F, Joyce, Catherine M

“…We report the first pre-steady-state kinetic studies of DNA replication in the absence of hydrogen bonds. We have used nonpolar nucleotide analogues that mimic…”
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Conformational landscapes of DNA polymerase I and mutator derivatives establish fidelity checkpoints for nucleotide insertion by Hohlbein, Johannes, Aigrain, Louise, Craggs, Timothy D., Bermek, Oya, Potapova, Olga, Shoolizadeh, Pouya, Grindley, Nigel D. F., Joyce, Catherine M., Kapanidis, Achillefs N.

“…The fidelity of DNA polymerases depends on conformational changes that promote the rejection of incorrect nucleotides before phosphoryl transfer. Here, we…”
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Implications of structures of synaptic tetramers of gamma delta resolvase for the mechanism of recombination by Kamtekar, Satwik, Ho, Roger S, Cocco, Melanie J, Li, Weikai, Wenwieser, Sandra V C T, Boocock, Martin R, Grindley, Nigel D F, Steitz, Thomas A

“…The structures of two mutants of the site-specific recombinase, gammadelta resolvase, that form activated tetramers have been determined. One, at 3.5-A…”
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Interaction of DNA Polymerase I (Klenow Fragment) with the Single-Stranded Template beyond the Site of Synthesis by Turner, Robert M, Grindley, Nigel D. F, Joyce, Catherine M

“…Cocrystal structures of DNA polymerases from the Pol I (or A) family have provided only limited information about the location of the single-stranded template…”
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Crossover-site sequence and DNA torsional stress control strand interchanges by the Bxb1 site-specific serine recombinase by Keenholtz, Ross A, Grindley, Nigel D F, Hatfull, Graham F, Marko, John F

“…DNA segment exchange by site-specific serine recombinases (SRs) is thought to proceed by rigid-body rotation of the two halves of the synaptic complex,…”
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Discrimination against Purine-Pyrimidine Mispairs in the Polymerase Active Site of DNA Polymerase I: A Structural Explanation by Minnick, Dana T., Liu, Lixing, Nigel D. F. Grindley, Kunkel, Thomas A., Joyce, Catherine M.

“…We previously identified five derivatives of Klenow fragment DNA polymerase that have lower fidelity because of amino acid substitutions in the polymerase…”
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Structure of a Synaptic γδ Resolvase Tetramer Covalently Linked to Two Cleaved DNAs by Li, Weikai, Kamtekar, Satwik, Xiong, Yong, Sarkis, Gary J., Nigel D. F. Grindley, Steitz, Thomas A.

“…The structure of a synaptic intermediate of the site-specific recombinase γ δ resolvase covalently linked through$Ser^{10}$to two cleaved duplex DNAs has been…”
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In Vitro Transposition System for Efficient Generation of Random Mutants of Campylobacter jejuni by Colegio, O R, Griffin, 4th, T J, Grindley, N D, Galán, J E

“…Article Usage Stats Services JB Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley…”
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