Search Results - "Griffiths, Andrew D"

High-throughput single-cell ChIP-seq identifies heterogeneity of chromatin states in breast cancer by Grosselin, Kevin, Durand, Adeline, Marsolier, Justine, Poitou, Adeline, Marangoni, Elisabetta, Nemati, Fariba, Dahmani, Ahmed, Lameiras, Sonia, Reyal, Fabien, Frenoy, Olivia, Pousse, Yannick, Reichen, Marcel, Woolfe, Adam, Brenan, Colin, Griffiths, Andrew D., Vallot, Céline, Gérard, Annabelle

“…Modulation of chromatin structure via histone modification is a major epigenetic mechanism and regulator of gene expression. However, the contribution of…”
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Single-cell analysis and sorting using droplet-based microfluidics by Mazutis, Linas, Gilbert, John, Ung, W Lloyd, Weitz, David A, Griffiths, Andrew D, Heyman, John A

“…We present a droplet-based microfluidics protocol for high-throughput analysis and sorting of single cells. Compartmentalization of single cells in droplets…”
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Emergence of a catalytic tetrad during evolution of a highly active artificial aldolase by Obexer, Richard, Godina, Alexei, Garrabou, Xavier, Mittl, Peer R. E., Baker, David, Griffiths, Andrew D., Hilvert, Donald

“…Designing catalysts that achieve the rates and selectivities of natural enzymes is a long-standing goal in protein chemistry. Here, we show that an…”
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Functional single-cell hybridoma screening using droplet-based microfluidics by Debs, Bachir El, Utharala, Ramesh, Balyasnikova, Irina V, Griffiths, Andrew D, Merten, Christoph A

“…Monoclonal antibodies can specifically bind or even inhibit drug targets and have hence become the fastest growing class of human therapeutics. Although they…”
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Enhanced chemical synthesis at soft interfaces: a universal reaction-adsorption mechanism in microcompartments by Fallah-Araghi, Ali, Meguellati, Kamel, Baret, Jean-Christophe, El Harrak, Abdeslam, Mangeat, Thomas, Karplus, Martin, Ladame, Sylvain, Marques, Carlos M, Griffiths, Andrew D

“…A bimolecular synthetic reaction (imine synthesis) was performed compartmentalized in micrometer-diameter emulsion droplets. The apparent equilibrium constant…”
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Monocyte Trajectories Endotypes Are Associated With Worsening in Septic Patients by Bodinier, Maxime, Peronnet, Estelle, Brengel-Pesce, Karen, Conti, Filippo, Rimmelé, Thomas, Textoris, Julien, Vedrine, Christophe, Quemeneur, Laurence, Griffiths, Andrew D, Tan, Lionel K, Venet, Fabienne, Maucort-Boulch, Delphine, Monneret, Guillaume

“…Sepsis is a life-threatening organ dysfunction caused by a dysregulated host response to infection. The immune system plays a key role in sepsis onset and…”
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Ultrahigh-throughput screening in drop-based microfluidics for directed evolution by Agresti, Jeremy J, Antipov, Eugene, Abate, Adam R, Ahn, Keunho, Rowat, Amy C, Baret, Jean-Christophe, Marquez, Manuel, Klibanov, Alexander M, Griffiths, Andrew D, Weitz, David A

“…The explosive growth in our knowledge of genomes, proteomes, and metabolomes is driving ever-increasing fundamental understanding of the biochemistry of life,…”
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Droplet-based microfluidic high-throughput screening of heterologous enzymes secreted by the yeast Yarrowia lipolytica by Beneyton, Thomas, Thomas, Stéphane, Griffiths, Andrew D, Nicaud, Jean-Marc, Drevelle, Antoine, Rossignol, Tristan

“…Droplet-based microfluidics is becoming an increasingly attractive alternative to microtiter plate techniques for enzymatic high-throughput screening (HTS),…”
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Transient compartmentalization of RNA replicators prevents extinction due to parasites by Matsumura, Shigeyoshi, Kun, Ádám, Ryckelynck, Michael, Coldren, Faith, Szilágyi, András, Jossinet, Fabrice, Rick, Christian, Nghe, Philippe, Szathmáry, Eörs, Griffiths, Andrew D.

“…The appearance of molecular replicators (molecules that can be copied) was probably a critical step in the originz of life. However, parasitic replicators…”
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Droplet-based microfluidics at the femtolitre scale by Leman, Marie, Abouakil, Faris, Griffiths, Andrew D, Tabeling, Patrick

“…We have built a toolbox of modules for droplet-based microfluidic operations on femtolitre volume droplets. We have demonstrated monodisperse production,…”
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A completely in vitro ultrahigh-throughput droplet-based microfluidic screening system for protein engineering and directed evolution by Fallah-Araghi, Ali, Baret, Jean-Christophe, Ryckelynck, Michael, Griffiths, Andrew D

“…In vitro screening systems based on the coupled transcription and translation of genes using cell-free systems have a number of attractive features for protein…”
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Multiplex picodroplet digital PCR to detect KRAS mutations in circulating DNA from the plasma of colorectal cancer patients by Taly, Valerie, Pekin, Deniz, Benhaim, Leonor, Kotsopoulos, Steve K, Le Corre, Delphine, Li, Xinyu, Atochin, Ivan, Link, Darren R, Griffiths, Andrew D, Pallier, Karine, Blons, Hélène, Bouché, Olivier, Landi, Bruno, Hutchison, J Brian, Laurent-Puig, Pierre

“…Multiplex digital PCR (dPCR) enables noninvasive and sensitive detection of circulating tumor DNA with performance unachievable by current molecular-detection…”
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High-throughput screening of filamentous fungi using nanoliter-range droplet-based microfluidics by Beneyton, Thomas, Wijaya, I. Putu Mahendra, Postros, Prexilia, Najah, Majdi, Leblond, Pascal, Couvent, Angélique, Mayot, Estelle, Griffiths, Andrew D., Drevelle, Antoine

“…Filamentous fungi are an extremely important source of industrial enzymes because of their capacity to secrete large quantities of proteins. Currently,…”
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Droplet-Based Microreactors for the Synthesis of Magnetic Iron Oxide Nanoparticles by Frenz, Lucas, El Harrak, Abdeslam, Pauly, Matthias, Bégin-Colin, Sylvie, Griffiths, Andrew D, Baret, Jean-Christophe

“…Microdroplets for nanoparticles: An extremely reliable method to create droplet pairs, based on hydrodynamic coupling of two spatially separated nozzles, has…”
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Dynamic single-cell phenotyping of immune cells using the microfluidic platform DropMap by Bounab, Yacine, Eyer, Klaus, Dixneuf, Sophie, Rybczynska, Magda, Chauvel, Cécile, Mistretta, Maxime, Tran, Trang, Aymerich, Nathan, Chenon, Guilhem, Llitjos, Jean-François, Venet, Fabienne, Monneret, Guillaume, Gillespie, Iain A., Cortez, Pierre, Moucadel, Virginie, Pachot, Alexandre, Troesch, Alain, Leissner, Philippe, Textoris, Julien, Bibette, Jérôme, Guyard, Cyril, Baudry, Jean, Griffiths, Andrew D., Védrine, Christophe

“…Characterization of immune responses is currently hampered by the lack of systems enabling quantitative and dynamic phenotypic characterization of individual…”
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Multiplex digital PCR: breaking the one target per color barrier of quantitative PCR by Zhong, Qun, Bhattacharya, Smiti, Kotsopoulos, Steven, Olson, Jeff, Taly, Valérie, Griffiths, Andrew D, Link, Darren R, Larson, Jonathan W

“…Quantitative polymerase chain reactions (qPCR) based on real-time PCR constitute a powerful and sensitive method for the analysis of nucleic acids. However, in…”
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Metabolic cost of rapid adaptation of single yeast cells by Woronoff, Gabrielle, Nghe, Philippe, Baudry, Jean, Boitard, Laurent, Braun, Erez, Griffiths, Andrew D., Bibette, Jerome

“…Cells can rapidly adapt to changing environments through nongenetic processes; however, the metabolic cost of such adaptation has never been considered. Here…”
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High-resolution dose—response screening using droplet-based microfluidics by Miller, Oliver J., Harrak, Abdeslam El, Mangeat, Thomas, Baret, Jean-Christophe, Frenz, Lucas, Debs, Bachir El, Mayot, Estelle, Samuels, Michael L., Rooney, Eamonn K., Dieu, Pierre, Galvan, Martin, Link, Darren R., Griffiths, Andrew D.

“…A critical early step in drug discovery is the screening of a chemical library. Typically, promising compounds are identified in a primary screen and then more…”
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The establishment of variant surface glycoprotein monoallelic expression revealed by single-cell RNA-seq of Trypanosoma brucei in the tsetse fly salivary glands by Hutchinson, Sebastian, Foulon, Sophie, Crouzols, Aline, Menafra, Roberta, Rotureau, Brice, Griffiths, Andrew D, Bastin, Philippe

“…The long and complex Trypanosoma brucei development in the tsetse fly vector culminates when parasites gain mammalian infectivity in the salivary glands. A key…”
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Using droplet-based microfluidics to improve the catalytic properties of RNA under multiple-turnover conditions by Ryckelynck, Michael, Baudrey, Stéphanie, Rick, Christian, Marin, Annick, Coldren, Faith, Westhof, Eric, Griffiths, Andrew D

“…In vitro evolution methodologies are powerful approaches to identify RNA with new functionalities. While Systematic Evolution of Ligands by Exponential…”
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