Comparative Analysis of Extracellular Vesicles from Cytotoxic CD8 + αβ T Cells and γδ T Cells

Although belonging to different branches of the immune system, cytotoxic CD8 αβ T cells and γδ T cells utilize common cytolytic effectors including FasL, granzymes, perforin and granulysin. The effector proteins are stored in different subsets of lysosome-related effector vesicles (LREVs) and releas...

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Bibliographic Details
Published in:	Cells (Basel, Switzerland) Vol. 13; no. 20; p. 1745
Main Authors:	Griesel, Lisa, Kaleja, Patrick, Tholey, Andreas, Lettau, Marcus, Janssen, Ottmar
Format:	Journal Article
Language:	English
Published:	Switzerland MDPI AG 21-10-2024 MDPI
Subjects:	Antibodies Blood & organ donations CD8 antigen CD8+ T cells CD8-Positive T-Lymphocytes - immunology CD8-Positive T-Lymphocytes - metabolism Cell culture Cell death Cell interactions Cloning Comparative analysis cytotoxic effector proteins cytotoxic granules cytotoxic T cells Cytotoxicity Effector cells Endopeptidases Extracellular vesicles Extracellular Vesicles - immunology Extracellular Vesicles - metabolism FasL protein Humans Immune system Immunological synapses International economic relations Lymphocytes Lymphocytes T Marking and tracking techniques Mass spectroscopy Microenvironments Nanoparticles Penicillin Perforin Proteins Proteomics Proteomics - methods Receptors, Antigen, T-Cell, alpha-beta - metabolism Receptors, Antigen, T-Cell, gamma-delta - immunology Receptors, Antigen, T-Cell, gamma-delta - metabolism Scientific equipment and supplies industry Serine proteinase T cells T-Lymphocytes, Cytotoxic - immunology T-Lymphocytes, Cytotoxic - metabolism Ultracentrifugation Western blotting αβ T cells γδ T cells United States Germany United States > US proteomics profiling γδ T cells CD8+ T cells extracellular vesicles (EVs) cytotoxic effector proteins cytotoxic granules exosomes lysosome-related effector vesicles (LREVs) cytotoxic T cells αβ T cells
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Summary:	Although belonging to different branches of the immune system, cytotoxic CD8 αβ T cells and γδ T cells utilize common cytolytic effectors including FasL, granzymes, perforin and granulysin. The effector proteins are stored in different subsets of lysosome-related effector vesicles (LREVs) and released to the immunological synapse upon target cell encounter. Notably, in activated cells, LREVs and potentially other vesicles are continuously produced and released as extracellular vesicles (EVs). Presumably, EVs serve as mediators of intercellular communication in the local microenvironment or at distant sites. EVs of activated and expanded cytotoxic CD8 αβ T cells or γδ T cells were enriched from culture supernatants by differential and ultracentrifugation and characterized by nanoparticle tracking analyses and Western blotting. For a comparative proteomic profiling, EV preparations from both cell types were isobaric labeled with tandem mass tags (TMT10plex) and subjected to mass spectrometry analysis. 686 proteins were quantified in EV preparations of cytotoxic CD8 αβ T cells and γδ T cells. Both populations shared a major set of similarly abundant proteins, while much fewer proteins presented higher abundance levels in either CD8 αβ T cells or γδ T cells. To our knowledge, we provide the first comparative analysis of EVs from cytotoxic CD8 αβ T cells and γδ T cells.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 These authors contributed equally to this work.
ISSN:	2073-4409 2073-4409
DOI:	10.3390/cells13201745