Peripheral blood gene expression profiles in metabolic syndrome, coronary artery disease and type 2 diabetes

To determine if individuals with metabolic disorders possess unique gene expression profiles, we compared transcript levels in peripheral blood from patients with coronary artery disease (CAD), type 2 diabetes (T2D) and their precursor state, metabolic syndrome to those of control (CTRL) subjects an...

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Bibliographic Details
Published in:	Genes and immunity Vol. 12; no. 5; pp. 341 - 351
Main Authors:	Grayson, B L, Wang, L, Aune, T M
Format:	Journal Article
Language:	English
Published:	London Nature Publishing Group UK 01-07-2011 Nature Publishing Group
Subjects:	631/208/191/2018 692/699/249/1313/498 692/699/2743/2037 692/699/75/593/15 Animal models Arthritis, Rheumatoid - genetics Autoimmune diseases Biomedical and Life Sciences Biomedicine Body fat Cancer Research Cardiovascular disease Cell activation Cluster Analysis Coronary artery Coronary Artery Disease - genetics Coronary heart disease Coronary vessels Cytokines Data processing Development and progression Diabetes Diabetes mellitus Diabetes mellitus (non-insulin dependent) Diabetes Mellitus, Type 2 - genetics Diagnosis DNA microarrays Gene Expression Gene Expression Profiling Gene Expression Regulation Genetic Predisposition to Disease - genetics Heart diseases Hemopoiesis Human Genetics Humans Immune response Immunology Inflammation Innate immunity Insulin resistance Lymphocytes T Medicine Metabolic disorders Metabolic syndrome Metabolic Syndrome - genetics NF- Kappa B protein NF-κB protein Obesity original-article Peripheral blood Physiological aspects Polymerase chain reaction Polymorphism, Single Nucleotide - genetics Reproducibility of Results Rheumatoid arthritis Risk factors RNA-directed DNA polymerase Stem cells Transcription Transcription activation Transcription factors Tumor necrosis factor-TNF Type 2 diabetes Vein & artery diseases United States United States > US Nashville Tennessee coronary artery disease metabolic syndrome type 2 diabetes gene set rheumatoid arthritis gene expression profiles
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Summary:	To determine if individuals with metabolic disorders possess unique gene expression profiles, we compared transcript levels in peripheral blood from patients with coronary artery disease (CAD), type 2 diabetes (T2D) and their precursor state, metabolic syndrome to those of control (CTRL) subjects and subjects with rheumatoid arthritis (RA). The gene expression profile of each metabolic state was distinguishable from CTRLs and correlated with other metabolic states more than with RA. Of note, subjects in the metabolic cohorts overexpressed gene sets that participate in the innate immune response. Genes involved in activation of the pro-inflammatory transcription factor, NF-κB, were overexpressed in CAD whereas genes differentially expressed in T2D have key roles in T-cell activation and signaling. Reverse transcriptase PCR validation confirmed microarray results. Furthermore, several genes differentially expressed in human metabolic disorders have been previously shown to participate in inflammatory responses in murine models of obesity and T2D. Taken together, these data demonstrate that peripheral blood from individuals with metabolic disorders display overlapping and non-overlapping patterns of gene expression indicative of unique, underlying immune processes.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 ObjectType-Article-2 ObjectType-Feature-1
ISSN:	1466-4879 1476-5470
DOI:	10.1038/gene.2011.13