Search Results - "Graumann, Peter"
-
1
Progress towards Bioorthogonal Catalysis with Organometallic Compounds
Published in Angewandte Chemie International Edition (22-09-2014)“…The catalysis of bioorthogonal transformations inside living organisms is a formidable challenge—yet bears great potential for future applications in chemical…”
Get full text
Journal Article -
2
Uptake of environmental DNA in Bacillus subtilis occurs all over the cell surface through a dynamic pilus structure
Published in PLoS genetics (10-10-2023)“…At the transition to stationary phase, a subpopulation of Bacillus subtilis cells can enter the developmental state of competence, where DNA is taken up…”
Get full text
Journal Article -
3
Dynamics of cell wall-binding proteins at a single molecule level: B. subtilis autolysins show different kinds of motion
Published in Molecular biology of the cell (01-04-2024)“…The bacterial cell wall is a meshwork of crosslinked peptidoglycan strands, with a thickness of up to 50 nm in Firmicutes. Little is known about how proteins…”
Get full text
Journal Article -
4
Molecular basis for lethal cross-talk between two unrelated bacterial transcription factors - the regulatory protein of a restriction-modification system and the repressor of a defective prophage
Published in Nucleic acids research (28-10-2022)“…Abstract Bacterial gene expression depends on the efficient functioning of global transcriptional networks, however their interconnectivity and orchestration…”
Get full text
Journal Article -
5
Superresolution Imaging of Dynamic MreB Filaments in B. subtilis—A Multiple-Motor-Driven Transport?
Published in Biophysical journal (03-09-2013)“…The cytoskeletal protein MreB is an essential component of the bacterial cell-shape generation system. Using a superresolution variant of total internal…”
Get full text
Journal Article -
6
Regulator-dependent temporal dynamics of a restriction-modification system's gene expression upon entering new host cells: single-cell and population studies
Published in Nucleic acids research (19-04-2021)“…Abstract Restriction-modification (R-M) systems represent a first line of defense against invasive DNAs, such as bacteriophage DNAs, and are widespread among…”
Get full text
Journal Article -
7
Single-molecule dynamics suggest that ribosomes assemble at sites of translation in Bacillus subtilis
Published in Frontiers in microbiology (03-11-2022)“…Eukaryotic cells transcribe ribosomal RNA and largely assemble ribosomes in a structure called the nucleolus, where chromosomal regions containing rRNA operons…”
Get full text
Journal Article -
8
Real-Time Messenger RNA Dynamics in Bacillus subtilis
Published in Frontiers in microbiology (18-11-2021)“…Messenger RNA molecules have been localized to different positions in cells and have been followed by time-lapse microscopy. We have used MS2-mVenus–labeled…”
Get full text
Journal Article -
9
Factors affecting mixed-mode retention properties of cation-exchange stationary phases
Published in Journal of Chromatography A (26-04-2023)“…•Application of cation-exchangers in multiple chromatographic modes.•Separation of alkylbenzenes, trialkylamines, nucleoside derivatives and Rb+ on single…”
Get full text
Journal Article -
10
Cytoskeletal elements in bacteria
Published in Annual review of microbiology (01-01-2007)“…All cytoskeletal elements known from eukaryotic cells are also present in bacteria, where they perform vital tasks in many aspects of the physiology of the…”
Get full text
Journal Article -
11
Single molecule/particle tracking analysis program SMTracker 2.0 reveals different dynamics of proteins within the RNA degradosome complex in Bacillus subtilis
Published in Nucleic acids research (08-11-2021)“…Abstract Single-molecule (particle) tracking is a powerful method to study dynamic processes in cells at highest possible spatial and temporal resolution. We…”
Get full text
Journal Article -
12
The ultimate picture—the combination of live cell superresolution microscopy and single molecule tracking yields highest spatio-temporal resolution
Published in Current opinion in microbiology (01-06-2018)“…•Advancements in non-diffraction limited microscopy techniques have improved our understanding of a wide variety of proteins and their functions in live…”
Get full text
Journal Article -
13
Substrate-dependent assembly of the Tat translocase as observed in live Escherichia coli cells
Published in PloS one (02-08-2013)“…The twin-arginine translocation (Tat) pathway guides fully folded proteins across membranes of bacteria, archaea and plant chloroplasts. In Escherichia coli,…”
Get full text
Journal Article -
14
Protein secretion zones during overexpression of amylase within the Gram-positive cell wall
Published in BMC biology (04-10-2023)“…Abstract Background Whereas the translocation of proteins across the cell membrane has been thoroughly investigated, it is still unclear how proteins cross the…”
Get full text
Journal Article -
15
Rapid turnover of DnaA at replication origin regions contributes to initiation control of DNA replication
Published in PLoS genetics (06-02-2017)“…DnaA is a conserved key regulator of replication initiation in bacteria, and is homologous to ORC proteins in archaea and in eukaryotic cells. The ATPase binds…”
Get full text
Journal Article -
16
Strain-dependent motility defects and suppression by a flhO mutation for B. subtilis bactofilins
Published in BMC research notes (13-05-2022)“…Bactofilins can assemble into polymeric structures and play important roles in cell shape maintenance, chromosome segregation and motility. Bacillus subtilis…”
Get full text
Journal Article -
17
Single-Molecule Dynamics of DNA Receptor ComEA, Membrane Permease ComEC, and Taken-Up DNA in Competent Bacillus subtilis Cells
Published in Journal of bacteriology (15-03-2022)“…In competent Gram-negative and Gram-positive bacteria, double-stranded DNA is taken up through the outer cell membrane and/or the cell wall and is bound by…”
Get full text
Journal Article -
18
SMTracker: a tool for quantitative analysis, exploration and visualization of single-molecule tracking data reveals highly dynamic binding of B. subtilis global repressor AbrB throughout the genome
Published in Scientific reports (24-10-2018)“…Single-particle (molecule) tracking (SPT/SMT) is a powerful method to study dynamic processes in living cells at high spatial and temporal resolution. Even…”
Get full text
Journal Article -
19
Visual Evidence for the Recruitment of Four Enzymes with RNase Activity to the Bacillus subtilis Replication Forks
Published in Cells (Basel, Switzerland) (01-08-2024)“…Removal of RNA/DNA hybrids for the maturation of Okazaki fragments on the lagging strand, or due to misincorporation of ribonucleotides by DNA polymerases, is…”
Get full text
Journal Article -
20
Microdomain formation is a general property of bacterial membrane proteins and induces heterogeneity of diffusion patterns
Published in BMC biology (03-09-2018)“…Proteins within the cytoplasmic membrane display distinct localization patterns and arrangements. While multiple models exist describing the dynamics of…”
Get full text
Journal Article