Development of hybridization probes on the basis of recombinant DNA to identify porcine parvoviruses and rotaviruses

Porcine parvovirus (PPV) and porcine rotavirus (PRV) infections can be diagnosed, and the diagnosis greatly contributes to their control. For the diagnosis of PPV and PRV of pigs recombinant DNA were obtained which could be used as hybridization probes. Homology between PPV-RNA and cloned cDNA was c...

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Bibliographic Details
Published in:	Archiv fur experimentelle Veterinarmedizin Vol. 44; no. 6; p. 897
Main Authors:	Koromyslov, G F, Artjushin, S K, Zaberezhny, A D, Grashuk, V N
Format:	Journal Article
Language:	English
Published:	Germany 1990
Subjects:	Animals DNA Probes Nucleic Acid Hybridization Parvoviridae - isolation & purification Parvoviridae Infections - diagnosis Parvoviridae Infections - veterinary Rotavirus - isolation & purification Rotavirus Infections - diagnosis Rotavirus Infections - veterinary Swine Swine Diseases - diagnosis
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Summary:	Porcine parvovirus (PPV) and porcine rotavirus (PRV) infections can be diagnosed, and the diagnosis greatly contributes to their control. For the diagnosis of PPV and PRV of pigs recombinant DNA were obtained which could be used as hybridization probes. Homology between PPV-RNA and cloned cDNA was confirmed, and the ability of plasmid probes to detect PRV was demonstrated. Hybridization with preparations of both PPV-infected cells and purified PPV gave positive reproducible results. The results are useful as an experience for the development of a sandwich hybridization system for PPV detection.
ISSN:	0003-9055