Search Results - "Grasby, J A"

Metal Ions Play a Passive Role in the Hairpin Ribozyme Catalysed Reaction by Young, Karen J., Gill, Francesca, Grasby, Jane A.

“…The hairpin ribozyme is an example of a small catalytic RNA which catalyses the endonucleolytic transesterification of RNA in a highly sequence-specific…”
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Methylphosphonate mapping of phosphate contacts critical for RNA recognition by the human immunodeficiency virus tat and rev proteins by Pritchard, Clare E., Grasby, Jane A., Hamy, François, Zacharek, Anthony M., Singh, Mohinder, Karn, Jonathan, Gait, Michael J.

“…The HIV-1 regulatory proteins tat and rev are both RNA binding proteins which recognize sequences in duplex RNA which are close to structural distortions. Here…”
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Stereochemical outcome of the hydrolysis reaction catalyzed by the EcoRV restriction endonuclease by Grasby, Jane A, Connolly, Bernard A

“…The stereochemical course of the reaction catalyzed by the EcoRV restriction endonuclease has been determined. This endonuclease recognizes GATATC sequence and…”
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The synthesis of oligoribonucleotides containing O6-methylguanosine: the role of conserved guanosine residues in hammerhead ribozyme cleavage by Grasby, Jane A., Jonathan, P., Butler, G., Michael, J.Gait

“…The synthesis is described of oligoribonucleotides containing the modified nucleoside O6-methylguanosine. Solid-phase oligoribonucleotide assembly was carried…”
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Enhancing the catalytic repertoire of nucleic acids. II. Simultaneous incorporation of amino and imidazolyl functionalities by two modified triphosphates during PCR by Gourlain, T, Sidorov, A, Mignet, N, Thorpe, S J, Lee, S E, Grasby, J A, Williams, D M

“…The incorporation of potentially catalytic groups into DNA is of interest for the in vitro selection of novel deoxyribozymes. We have devised synthetic routes…”
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Enhancing the catalytic repertoire of nucleic acids: a systematic study of linker length and rigidity by Lee, S E, Sidorov, A, Gourlain, T, Mignet, N, Thorpe, S J, Brazier, J A, Dickman, M J, Hornby, D P, Grasby, J A, Williams, D M

“…The incorporation of potentially catalytic groups in DNA is of interest for the in vitro selection of novel deoxyribozymes. A series of 10 C5-modified…”
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Synthetic oligoribonucleotides carrying site-specific modifications for RNA structure-function analysis by Grasby, J A, Gait, M J

“…Synthetic oligoribonucleotides have become increasingly valuable in studies of RNA structure and function. A range of nucleotide analogues is available which…”
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Influence of the phosphate backbone on the recognition and hydrolysis of DNA by the EcoRV restriction endonuclease. A study using oligodeoxynucleotide phosphorothioates by Thorogood, H, Grasby, J A, Connolly, B A

“…A set of phosphorothioate-containing oligonucleotides based on pGACGATATCGTC, a self-complementary dodecamer that contains the EcoRV recognition sequence…”
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Purine Functional Groups in Essential Residues of the Hairpin Ribozyme Required for Catalytic Cleavage of RNA by Grasby, Jane A, Mersmann, Karin, Singh, Mohinder, Gait, Michael J

“…Synthetic chemistry techniques have been used to study the functional group requirements of the essential purine residues in hairpin ribozyme cleavage…”
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A single cleavage assay for T5 5′→3′ exonuclease: Determination of the catalytic parameters for wild-type and mutant proteins by Pickering, Timothy J., Thorpe, Simon J., Grasby, Jane A., Garforth, Scott J., Sayers, Jon R.

“…Bacteriophage T5 5′→3′ exonuclease is a member of a family of sequence related 5′-nucleases which play an essential role in DNA replication. The 5′-nucleases…”
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The role of essential pyrimidines in the hairpin ribozyme-catalysed reaction by Young, Karen J., Vyle, Joseph S., Pickering, Timothy J., Cohen, Mark A., Holmes, Stephen C., Merkel, Olaf, Grasby, Jane A.

“…The hairpin ribozyme is an example of a small catalytic RNA that catalyses the endonucleolytic transesterification of RNA in a highly sequence-specific manner…”
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Variation in the steady state kinetic parameters of wild type and mutant T5 5'-3'-exonuclease with pH. Protonation of Lys-83 is critical for DNA binding by Pickering, T J, Garforth, S, Sayers, J R, Grasby, J A

“…T5 5'-3'-exonuclease is a member of a family of homologous 5'-nucleases essential for DNA replication and repair. We have measured the variation of the steady…”
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Sequence‐specific cleavage of RNA in the absence of divalent metal ions by a DNAzyme incorporating imidazolyl and amino functionalities by Sidorov, Alexander V., Grasby, Jane A., Williams, David M.

“…Two modified 2′‐deoxynucleoside 5′‐triphosphates have been used for the in vitro selection of a modified deoxyribozyme (DNAzyme) capable of the…”
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The pH-dependence of the Escherichia coli RNase HII-catalysed Reaction Suggests that an Active Site Carboxylate Group Participates Directly in Catalysis by Bastock, James A., Webb, Michelle, Grasby, Jane A.

“…RNase HII specifically catalyses the hydrolysis of phosphate diester linkages contained within the RNA portion of DNA/RNA hybrids. The catalytic parameters of…”
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Comparison of the Catalytic Parameters and Reaction Specificities of a Phage and an Archaeal Flap Endonuclease by Williams, Ryan, Sengerová, Blanka, Osborne, Sadie, Syson, Karl, Ault, Sophie, Kilgour, Anna, Chapados, Brian R., Tainer, John A., Sayers, Jon R., Grasby, Jane A.

“…Flap endonucleases (FENs) catalyse the exonucleolytic hydrolysis of blunt-ended duplex DNA substrates and the endonucleolytic cleavage of 5′-bifurcated nucleic…”
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Determination of the Catalytic Parameters of the N-terminal Half of Escherichia coli Ribonuclease E and the Identification of Critical Functional Groups in RNA Substrates by Redko, Yulia, Tock, Mark R., Adams, Chris J., Kaberdin, Vladimir R., Grasby, Jane A., McDowall, Kenneth J.

“…Ribonuclease E is required for the rapid decay and correct processing of RNA in Escherichia coli. A detailed understanding of the hydrolysis of RNA by this and…”
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Dynamic evidence for metal ion catalysis in the reaction mediated by a flap endonuclease by Tock, Mark R., Frary, Elaine, Sayers, Jon R., Grasby, Jane A.

“…On the basis of structural work, metal ions are proposed to play a catalytic role in reactions mediated by many phosphoryl transfer enzymes. To gain dynamic…”
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A single cleavage assay for T5 5'-->3' exonuclease: determination of the catalytic parameters forwild-type and mutant proteins by Pickering, T J, Garforth, S J, Thorpe, S J, Sayers, J R, Grasby, J A

“…Bacteriophage T5 5'-->3' exonuclease is a member of a family of sequence related 5'-nucleases which play an essential role in DNA replication. The 5'-nucleases…”
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The synthesis and properties of oligoribonucleotide-spermine conjugates by Marsh, Andrew J, Williams, David M, Grasby, Jane A

“…Polyamines stabilise nucleic acids against chemical and enzymatic degradation, facilitate the formation of secondary and tertiary structures and enhance…”
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The Role of Magnesium Ions and 2′-Hydroxyl Groups in the VS Ribozyme–Substrate Interaction by Tzokov, Svetomir B, Murray, Iain A, Grasby, Jane A

“…The minimal substrate of the trans-cleaving Neurospora VS ribozyme has a stem-loop structure and interacts with the ribozyme by RNA tertiary interactions that…”
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