Use of Zebrafish (Danio rerio) for Biosafety Evaluation of Strontium Nanostructured Hydroxyapatite

ABSTRACT Despite the numerous studies on biocompatibility with nano‐biomaterials, the biological effects of strontium‐substituted HA nanoparticles (nSrHA) need to be better understood. So, we conducted an embryotoxicity test using zebrafish (Danio rerio) according to the OECD 236 guideline, a model...

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Published in:	Journal of biomedical materials research. Part B, Applied biomaterials Vol. 112; no. 9; pp. e35478 - n/a
Main Authors:	Costa Silva, Elisângela, Souza, Augusto Monteiro, Rossi, Alexandre Malta, Costa, Andrea Machado, Grangeiro, José Mauro, Luchiari, Ana Carolina, Medeiros, Silvia Regina Batistuzzo
Format:	Journal Article
Language:	English
Published:	Hoboken, USA John Wiley & Sons, Inc 01-09-2024 Wiley Subscription Services, Inc
Subjects:	Animals Biocompatibility Biological effects Biomaterials Biomedical materials Biosafety Bone biomaterials Danio rerio Developmental stages embryo biosafety Embryo, Nonmammalian - drug effects Embryos Hydroxyapatite Hydroxyapatites - chemistry Hydroxyapatites - pharmacology Larva - drug effects Larval development Lethality Materials Testing Materials traceability Microspheres Nanoparticles Nanostructure nanostructured biomaterials Nanostructures - chemistry Neurotoxicity Reactive oxygen species Reactive Oxygen Species - metabolism Strontium Strontium - chemistry Strontium - pharmacology Toxicity testing Zebrafish hydroxyapatite embryo biosafety nanostructured biomaterials strontium
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Summary:	ABSTRACT Despite the numerous studies on biocompatibility with nano‐biomaterials, the biological effects of strontium‐substituted HA nanoparticles (nSrHA) need to be better understood. So, we conducted an embryotoxicity test using zebrafish (Danio rerio) according to the OECD 236 guideline, a model that represents a viable alternative that bridges the gap between in vitro and mammalian models. Zebrafish embryos were exposed for 120 h to microspheres containing nSrHA nanoparticles with low and high crystallinity, synthesized at temperatures of 5°C (nSrHA5) and 90°C (nSrHA90). We evaluated lethality, developmental parameters, and reactive oxygen species (ROS) production. The larval behavior was assessed at 168 hpf to determine if the biomaterials affected motor responses and anxiety‐like behavior. The results showed that the survival rate decreased significantly for the nSrHA5 group (low crystalline particles), and an increase in ROS was also observed in this group. However, none of the biomaterials caused morphological changes indicative of toxicity during larval development. Additionally, the behavioral tests did not reveal any alterations in all experimental groups, indicating the absence of neurotoxic effects from exposure to the tested biomaterials. These findings provide valuable insights into the biosafety of modified HA‐based nanostructured biomaterials, making them a promising strategy for bone tissue repair. As the use of hydroxyapatite‐based biomaterials continues to grow, it is crucial to ensure rigorous control over the quality, reliability, and traceability of these materials.
Bibliography:	Funding This work was supported by Instituto Nacional de Ciência e Tecnologia em Medicina Regenerativa, Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), and Conselho Nacional de Desenvolvimento Científico e Tecnológico. Elisângela da Costa Silva and Augusto Monteiro de Souza contributed equally to this work. ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1552-4973 1552-4981 1552-4981
DOI:	10.1002/jbm.b.35478