Search Results - "Grahame, David A"

Diverse Energy-Conserving Pathways in Clostridium difficile: Growth in the Absence of Amino Acid Stickland Acceptors and the Role of the Wood-Ljungdahl Pathway by Gencic, Simonida, Grahame, David A

“…is the leading cause of hospital-acquired antibiotic-associated diarrhea and is the only widespread human pathogen that contains a complete set of genes…”
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Capturing a methanogenic carbon monoxide dehydrogenase/acetyl-CoA synthase complex via cryogenic electron microscopy by Biester, Alison, Grahame, David A, Drennan, Catherine L

“…Approximately two-thirds of the estimated one-billion metric tons of methane produced annually by methanogens is derived from the cleavage of acetate. Acetate…”
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The two-electron reduced A cluster in acetyl-CoA synthase: Preparation, characteristics and mechanistic implications by Gencic, Simonida, Duin, Evert C., Grahame, David A.

“…Acetyl-CoA synthase (ACS) is a central enzyme in the carbon and energy metabolism of certain anaerobic species of bacteria and archaea that catalyzes the…”
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Methods for analysis of acetyl-CoA synthase applications to bacterial and archaeal systems by Grahame, David A

“…The nickel- and iron-containing enzyme acetyl-CoA synthase (ACS) catalyzes de novo synthesis as well as overall cleavage of acetyl-CoA in acetogens, various…”
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Tight Coupling of Partial Reactions in the Acetyl-CoA Decarbonylase/Synthase (ACDS) Multienzyme Complex from Methanosarcina thermophila: ACETYL C-C BOND FRAGMENTATION AT THE A CLUSTER PROMOTED BY PROTEIN CONFORMATIONAL CHANGES by Gencic, Simonida, Duin, Evert C, Grahame, David A

“…Direct synthesis and cleavage of acetyl-CoA are carried out by the bifunctional CO dehydrogenase/acetyl-CoA synthase enzyme in anaerobic bacteria and by the…”
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Two Separate One-Electron Steps in the Reductive Activation of the A Cluster in Subunit β of the ACDS Complex in Methanosarcina thermophila by Gencic, Simonida, Grahame, David A

“…Acetyl-CoA decarbonylase/synthase (ACDS) is a multienzyme complex found in methanogens and certain other Archaea that carries out the overall synthesis and…”
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Different Modes of Carbon Monoxide Binding to Acetyl-CoA Synthase and the Role of a Conserved Phenylalanine in the Coordination Environment of Nickel by Gencic, Simonida, Kelly, Kayla, Ghebreamlak, Selamawit, Duin, Evert C, Grahame, David A

“…Acetyl-CoA synthase (ACS) catalyzes the reversible condensation of CO and CH3 units at a unique Ni–Fe cluster, the A cluster, to form an acetyl-Ni intermediate…”
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The genome of M. acetivorans reveals extensive metabolic and physiological diversity by Galagan, James E, Nusbaum, Chad, Roy, Alice, Endrizzi, Matthew G, Macdonald, Pendexter, FitzHugh, Will, Calvo, Sarah, Engels, Reinhard, Smirnov, Serge, Atnoor, Deven, Brown, Adam, Allen, Nicole, Naylor, Jerome, Stange-Thomann, Nicole, DeArellano, Kurt, Johnson, Robin, Linton, Lauren, McEwan, Paul, McKernan, Kevin, Talamas, Jessica, Tirrell, Andrea, Ye, Wenjuan, Zimmer, Andrew, Barber, Robert D, Cann, Isaac, Graham, David E, Grahame, David A, Guss, Adam M, Hedderich, Reiner, Ingram-Smith, Cheryl, Kuettner, H Craig, Krzycki, Joseph A, Leigh, John A, Li, Weixi, Liu, Jinfeng, Mukhopadhyay, Biswarup, Reeve, John N, Smith, Kerry, Springer, Timothy A, Umayam, Lowell A, White, Owen, White, Robert H, Conway de Macario, Everly, Ferry, James G, Jarrell, Ken F, Jing, Hua, Macario, Alberto J L, Paulsen, Ian, Pritchett, Matthew, Sowers, Kevin R, Swanson, Ronald V, Zinder, Steven H, Lander, Eric, Metcalf, William W, Birren, Bruce

“…Methanogenesis, the biological production of methane, plays a pivotal role in the global carbon cycle and contributes significantly to global warming. The…”
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Nickel in subunit beta of the acetyl-CoA decarbonylase/synthase multienzyme complex in methanogens. Catalytic properties and evidence for a binuclear Ni-Ni site by Gencic, Simonida, Grahame, David A

“…The acetyl-CoA decarbonylase/synthase (ACDS) complex catalyzes the central reaction of acetyl C-C bond cleavage in methanogens growing on acetate and is also…”
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Substrate and Accessory Protein Requirements and Thermodynamics of Acetyl-CoA Synthesis and Cleavage in Methanosarcina barkeri by Grahame, David A, DeMoll, Edward

“…Enzymological studies on the multienzyme acetyl-CoA decarbonylase synthase (ACDS) complex from Methanosarcina barkeri have been conducted in order to identify…”
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Acetate C–C bond formation and decomposition in the anaerobic world: the structure of a central enzyme and its key active-site metal cluster by Grahame, David A.

“…The structure of carbon monoxide dehydrogenase/acetyl-coenzyme A synthase (CODH/ACS), a central enzyme in the anaerobic metabolism of acetyl-coenzyme A…”
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Tight Coupling of Partial Reactions in the Acetyl-CoA Decarbonylase/Synthase (ACDS) Multienzyme Complex from Methanosarcina thermophila by Gencic, Simonida, Duin, Evert C., Grahame, David A.

“…Direct synthesis and cleavage of acetyl-CoA are carried out by the bifunctional CO dehydrogenase/acetyl-CoA synthase enzyme in anaerobic bacteria and by the…”
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The A-Cluster in Subunit β of the Acetyl-CoA Decarbonylase/Synthase Complex from Methanosarcina thermophila: Ni and Fe K-Edge XANES and EXAFS Analyses by Gu, Weiwei, Gencic, Simonida, Cramer, Stephen P, Grahame, David A

“…The acetyl-CoA decarbonylase/synthase (ACDS) complex catalyzes the cleavage of acetyl-CoA in methanogens that metabolize acetate to CO2 and CH4, and also…”
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Chemically Distinct Ni Sites in the A-Cluster in Subunit β of the Acetyl-CoA Decarbonylase/Synthase Complex from Methanosarcina thermophila:  Ni L-Edge Absorption and X-ray Magnetic Circular Dichroism Analyses by FUNK, Tobias, GU, Weiwei, FRIEDRICH, Stephan, WANG, Hongxin, GENCIC, Simonida, GRAHAME, David A., CRAMER, Stephen P.

“…The 5-subunit-containing acetyl-CoA decarbonylase/synthase (ACDS) complex plays an important role in methanogenic Archaea that convert acetate to methane, by…”
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single operon-encoded form of the acetyl-CoA decarbonylase/synthase multienzyme complex responsible for synthesis and cleavage of acetyl-CoA in Methanosarcina thermophila by Grahame, David A, Gencic, Simonida, DeMoll, Edward

“…Methanogens growing on C-1 substrates synthesize 2-carbon acetyl groups in the form of acetyl-CoA for carbon assimilation using the multienzyme complex…”
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Oxoammonium Cation Intermediate in the Nitroxide-Catalyzed Dismutation of Superoxide by Krishna, Murali C., Grahame, David A., Samuni, Amram, Mitchell, James B., Russo, Angelo

“…Dismutation of superoxide has been shown previously to be catalyzed by stable nitroxide compounds. In the present study, the mechanism of superoxide (·O- 2)…”
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Redox-Dependent Acetyl Transfer Partial Reaction of the Acetyl-CoA Decarbonylase/Synthase Complex:  Kinetics and Mechanism by Bhaskar, B, DeMoll, Edward, Grahame, David A

“…Acetyl-CoA decarbonylase/synthase (ACDS) is a multienzyme complex that plays a central role in energy metabolism in Methanosarcina barkeri grown on acetate…”
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Partial Reactions Catalyzed by Protein Components of the Acetyl-CoA Decarbonylase Synthase Enzyme Complex from Methanosarcina barkeri by Grahame, D A, DeMoll, E

“…In methanogens, the acetyl-CoA decarbonylase synthase (ACDS) complex, which has five different subunits, catalyzes synthesis and cleavage of acetyl-CoA…”
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Chemically Distinct Ni Sites in the A-Cluster in Subunit β of the Acetyl-CoA Decarbonylase/Synthase Complex from Methanosarcina t hermophila:  Ni L-Edge Absorption and X-ray Magnetic Circular Dichroism Analyses by Funk, Tobias, Gu, Weiwei, Friedrich, Stephan, Wang, Hongxin, Gencic, Simonida, Grahame, David A, Cramer, Stephen P

“…The 5-subunit-containing acetyl-CoA decarbonylase/synthase (ACDS) complex plays an important role in methanogenic Archaea that convert acetate to methane, by…”
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Zinc−Thiolate Intermediate in Catalysis of Methyl Group Transfer in Methanosarcina barkeri by Gencic, Simonida, LeClerc, Gilles M, Gorlatova, Natalia, Peariso, Katrina, Penner-Hahn, James E, Grahame, David A

“…Methyl group transfer reactions are essential in methane-forming pathways in all methanogens. The involvement of zinc in catalysis of methyl group transfer was…”
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