Search Results - "Grace, Christy R.R."

Mechanism of Polyubiquitination by Human Anaphase-Promoting Complex: RING Repurposing for Ubiquitin Chain Assembly by Brown, Nicholas G., Watson, Edmond R., Weissmann, Florian, Jarvis, Marc A., VanderLinden, Ryan, Grace, Christy R.R., Frye, Jeremiah J., Qiao, Renping, Dube, Prakash, Petzold, Georg, Cho, Shein Ei, Alsharif, Omar, Bao, Ju, Davidson, Iain F., Zheng, Jie J., Nourse, Amanda, Kurinov, Igor, Peters, Jan-Michael, Stark, Holger, Schulman, Brenda A.

“…Polyubiquitination by E2 and E3 enzymes is a predominant mechanism regulating protein function. Some RING E3s, including anaphase-promoting complex/cyclosome…”
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A Dual E3 Mechanism for Rub1 Ligation to Cdc53 by Scott, Daniel C., Monda, Julie K., Grace, Christy R.R., Duda, David M., Kriwacki, Richard W., Kurz, Thimo, Schulman, Brenda A.

“…In ubiquitin-like protein (UBL) cascades, a thioester-linked E2∼UBL complex typically interacts with an E3 enzyme for UBL transfer to the target. Here we…”
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A RING E3-substrate complex poised for ubiquitin-like protein transfer: structural insights into cullin-RING ligases by Calabrese, Matthew F, Schulman, Brenda A, Scott, Daniel C, Duda, David M, Grace, Christy R R, Kurinov, Igor, Kriwacki, Richard W

“…How RING E3 ligases mediate E2-to-substrate ubiquitin-like protein (UBL) transfer remains unknown. Here we address how the RING E3 RBX1 positions NEDD8's E2…”
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Alteration of RNA Splicing by Small-Molecule Inhibitors of the Interaction between NHP2L1 and U4 by Diouf, Barthelemy, Lin, Wenwei, Goktug, Asli, Grace, Christy R R, Waddell, Michael Brett, Bao, Ju, Shao, Youming, Heath, Richard J, Zheng, Jie J, Shelat, Anang A, Relling, Mary V, Chen, Taosheng, Evans, William E

“…Splicing is an important eukaryotic mechanism for expanding the transcriptome and proteome, influencing a number of biological processes. Understanding its…”
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Distinct Structural and Functional Roles of Conserved Residues in the First Extracellular Domain of Receptors for Corticotropin-releasing Factor and Related G-protein-coupled Receptors by Perrin, Marilyn H., Grace, Christy R.R., DiGruccio, Michael R., Fischer, Wolfgang H., Maji, Samir K., Cantle, Jeffrey P., Smith, Sean, Manning, Gerard, Vale, Wylie W., Riek, Roland

“…The G-protein-coupled receptor B1 family includes corticotropin-releasing factor (CRF), growth hormone-releasing hormone, incretin, and pituitary adenylate…”
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A switch element in the autophagy E2 Atg3 mediates allosteric regulation across the lipidation cascade by Zheng, Yumei, Qiu, Yu, Grace, Christy R. R., Liu, Xu, Klionsky, Daniel J., Schulman, Brenda A.

“…Autophagy depends on the E2 enzyme, Atg3, functioning in a conserved E1-E2-E3 trienzyme cascade that catalyzes lipidation of Atg8-family ubiquitin-like…”
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Dual RING E3 Architectures Regulate Multiubiquitination and Ubiquitin Chain Elongation by APC/C by Brown, Nicholas G., VanderLinden, Ryan, Watson, Edmond R., Weissmann, Florian, Ordureau, Alban, Wu, Kuen-Phon, Zhang, Wei, Yu, Shanshan, Mercredi, Peter Y., Harrison, Joseph S., Davidson, Iain F., Qiao, Renping, Lu, Ying, Dube, Prakash, Brunner, Michael R., Grace, Christy R.R., Miller, Darcie J., Haselbach, David, Jarvis, Marc A., Yamaguchi, Masaya, Yanishevski, David, Petzold, Georg, Sidhu, Sachdev S., Kuhlman, Brian, Kirschner, Marc W., Harper, J. Wade, Peters, Jan-Michael, Stark, Holger, Schulman, Brenda A.

“…Protein ubiquitination involves E1, E2, and E3 trienzyme cascades. E2 and RING E3 enzymes often collaborate to first prime a substrate with a single ubiquitin…”
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RING E3 mechanism for ubiquitin ligation to a disordered substrate visualized for human anaphase-promoting complex by Brown, Nicholas G., VanderLinden, Ryan, Watson, Edmond R., Qiao, Renping, Grace, Christy R. R., Yamaguchi, Masaya, Weissmann, Florian, Frye, Jeremiah J., Dube, Prakash, Cho, Shein Ei, Actis, Marcelo L., Rodrigues, Patrick, Fujii, Naoaki, Peters, Jan-Michael, Stark, Holger, Schulman, Brenda A.

“…Significance The anaphase-promoting complex/cyclosome (APC) is a multisubunit RING E3 ubiquitin (Ub) ligase that regulates mitosis, meiosis, and numerous…”
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Allosteric regulation through a switch element in the autophagy E2, Atg3 by Qiu, Yu, Zheng, Yumei, Grace, Christy R. R., Liu, Xu, Klionsky, Daniel J., Schulman, Brenda A.

“…Lipidation of Atg8-family ubiquitin-like proteins (UBLs) plays important roles in macroautophagy/autophagy. This process is catalyzed by an E1-E2-E3 trienzyme…”
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Protein engineering of a ubiquitin-variant inhibitor of APC/C identifies a cryptic K48 ubiquitin chain binding site by Watson, Edmond R., Grace, Christy R. R., Zhang, Wei, Miller, Darcie J., Davidson, Iain F., Prabu, J. Rajan, Yu, Shanshan, Bolhuis, Derek L., Kulko, Elizaveta T., Vollrath, Ronnald, Haselbach, David, Stark, Holger, Peters, Jan-Michael, Brown, Nicholas G., Sidhu, Sachdev S., Schulman, Brenda A.

“…Ubiquitin (Ub)-mediated proteolysis is a fundamental mechanism used by eukaryotic cells to maintain homeostasis and protein quality, and to control timing in…”
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Structural insights into the role of the Smoothened cysteine-rich domain in Hedgehog signalling by Rana, Rajashree, Carroll, Candace E., Lee, Ho-Jin, Bao, Ju, Marada, Suresh, Grace, Christy R.R., Guibao, Cristina D., Ogden, Stacey K., Zheng, Jie J.

“…Smoothened (Smo) is a member of the Frizzled (FzD) class of G-protein-coupled receptors (GPCRs), and functions as the key transducer in the Hedgehog (Hh)…”
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NMR Structure and Peptide Hormone Binding Site of the First Extracellular Domain of a Type B1 G Protein-Coupled Receptor by Christy R. R. Grace, Perrin, Marilyn H., DiGruccio, Michael R., Miller, Charleen L., Rivier, Jean E., Vale, Wylie W., Riek, Roland

“…The corticotropin-releasing factor (CRF) ligand family has diverse effects on the CNS, including the modulation of the stress response. The ligands' effects…”
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Electron microscopy structure of human APC/[C.sup.CDH1]-EMI1 reveals multimodal mechanism of E3 ligase shutdown by Frye, Jeremiah J, Brown, Nicholas G, Petzold, Georg, Watson, Edmond R, Grace, Christy R.R, Nourse, Amanda, Jarvis, Marc A, Kriwacki, Richard W, Peters, Jan-Michael, Stark, Holger, Schulman, Brenda A

“…The anaphase-promoting complex/cyclosome (APC/C) is a ~1.5-MDa multiprotein E3 ligase enzyme that regulates cell division by promoting timely…”
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The three-dimensional structure of the N-terminal domain of corticotropin-releasing factor receptors: sushi domains and the B1 family of G protein-coupled receptors by Perrin, Marilyn H, Grace, Christy R R, Riek, Roland, Vale, Wylie W

“…The corticotropin-releasing factor (CRF) receptors, CRF-R1 and CRF-R2, belong to the B1 subfamily of G protein-coupled Receptors (GPCRs), including receptors…”
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Unique helical conformation of the fourth cytoplasmic loop of the CB1 cannabinoid receptor in a negatively charged environment by Grace, Christy R R, Cowsik, Sudha M, Shim, Joong-Youn, Welsh, William J, Howlett, Allyn C

“…The proximal portion of the C-terminus of the CB(1) cannabinoid receptor is a primary determinant for G-protein activation. A 17 residue proximal C-terminal…”
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Unique helical conformation of the fourth cytoplasmic loop of the CB 1 cannabinoid receptor in a negatively charged environment by Grace, Christy R.R., Cowsik, Sudha M., Shim, Joong-Youn, Welsh, William J., Howlett, Allyn C.

“…The proximal portion of the C-terminus of the CB 1 cannabinoid receptor is a primary determinant for G-protein activation. A 17 residue proximal C-terminal…”
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NMR techniques for very large proteins and rnas in solution by Tzakos, Andreas G, Grace, Christy R R, Lukavsky, Peter J, Riek, Roland

“…Three-dimensional structure determination of small proteins and oligonucleotides by solution NMR is established. With the development of novel NMR and labeling…”
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