Search Results - "Goward, C R"

Malate dehydrogenase: A model for structure, evolution, and catalysis by Goward, Christopher R., Nicholls, David J.

“…Malate dehydrogenases are widely distributed and alignment of the amino acid sequences show that the enzyme has diverged into 2 main phylogenetic groups…”
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Purification and properties of DNA polymerase from Bacillus caldotenax by BURROWS, J. A, GOWARD, C. R

“…A thermostable DNA polymerase was prepared from Bacillus caldotenax by using a four-step chromatography procedure. The protein exists as a monomer of M(r)…”
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The importance of arginine 102 for the substrate specificity of Escherichia coli malate dehydrogenase by Nicholls, D J, Miller, J, Scawen, M D, Clarke, A R, Holbrook, J J, Atkinson, T, Goward, C R

“…The malate dehydrogenase from Escherichia coli has been specifically altered at a single amino acid residue by using site-directed mutagenesis. The conserved…”
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The secondary structure of protein G', a robust molecule by GOWARD, C. R, IRONS, L. I, MURPHY, J. P, ATKINSON, T

“…The secondary structure of recombinant streptococcal Protein G' was predicted and compared with spectropolarimetric data. The predicted secondary structure…”
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The functional units of a peptostreptococcal protein L by Murphy, J P, Duggleby, C J, Atkinson, M A, Trowern, A R, Atkinson, T, Goward, C R

“…Protein L is a cell-surface protein from Peptostreptococcus which interacts with immunoglobulin kappa light chains. A gene from Peptostreptococcus strain 3316…”
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Expression and purification of a truncated recombinant streptococcal protein G by Goward, C R, Murphy, J P, Atkinson, T, Barstow, D A

“…The gene for Protein G from Streptococcus strain G148 was cloned and expressed in Escherichia coli. The regions on the gene corresponding to the…”
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Preparation of DNA polymerase from Bacillus caldotenax by Burrows, J A, Goward, C R

“…A procedure with four chromatography steps was developed for the purification of DNA polymerase from Bacillus caldotenax by using fast protein liquid…”
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The purification and characterization of glucokinase from the thermophile Bacillus stearothermophilus by Goward, C R, Hartwell, R, Atkinson, T, Scawen, M D

“…Homogeneous glucokinase (EC 2.7.1.2) from the thermophile Bacillus stearothermophilus was isolated on the large scale by using four major steps: precipitation…”
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Colorimetric glucose assay using thermostable glucokinase by Scott, D A, Goward, C R, Scawen, M D, Atkinson, T, Price, C P

“…A method for assaying glucose in serum or plasma samples using a thermostable glucokinase was developed. Glucokinase from Bacillus Stearothermophilus was…”
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The inhibition of glucokinase and glycerokinase from Bacillus stearothermophilus by the triazine dye Procion Blue MX-3G by Goward, C R, Scawen, M D, Atkinson, T

“…Glucokinase from Bacillus stearothermophilus was irreversibly inactivated by the reactive dichlorotriazinyl dye Procion Blue MX-3G at pH 8.0. The enzyme was…”
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Rapid purification of glucokinase and glycerokinase from Bacillus stearothermophilus by hydrophobic interaction chromatography by Goward, C R, Atkinson, T, Scawen, M D

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Molecular evolution of bacterial cell-surface proteins by Goward, C R, Scawen, M D, Murphy, J P, Atkinson, T

“…The cell-surface proteins of the infective bacteria Streptococcus and Staphylococcus are probably involved in the process of infection. These proteins share…”
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Rapid large-scale preparation of recombinant Erwinia chrysanthemi L-asparaginase by Goward, C R, Stevens, G B, Tattersall, R, Atkinson, T

“…L-asparaginase from Erwinia provides an alternative to the enzyme from E. coli for the effective treatment of acute lymphoblastic leukaemia. A procedure was…”
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Sequential 1H NMR assignments and secondary structure of an IgG-binding domain from protein G by LIAN, L.-Y, YANG, J. C, DERRICK, J. P, SUTCLIFFE, M. J, ROBERTS, G. C. K, MURPHY, J. P, GOWARD, C. R, ATKINSON, T

“…Protein G is a member of a class of cell surface bacterial proteins from Streptococcus that bind IgG with high affinity. A fragment of molecular mass 6988,…”
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Sequential proton NMR assignments and secondary structure of an IgG-binding domain from protein G by Lian, L. Y, Yang, J. C, Derrick, J. P, Sutcliffe, M. J, Roberts, G. C. K, Murphy, J. P, Goward, C. R, Atkinson, T

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Crystallization of cytochrome b562 from Erwinia chrysanthemi by Wilkinson, K. W., Ford, G. C., Moir, A. J., Rice, D. W., Rodgers, H. F., Smith, J. M. A., Stillman, T. J., Goward, C. R.

“…Cytochrome b562 from Erwinia chrysanthemi has been crystallized using the hanging‐drop vapour‐diffusion method with ammonium sulfate as the precipitant. X‐ray…”
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Amplified expression and large-scale purification of protein G by Murphy, J P, Atkinson, T, Hartwell, R, Stevens, G B, Hinton, R J, Goward, C R

“…PCR was used to isolate the gene fragment coding for Protein G' (SpG'), a truncated bacterial cell surface protein from Streptococcus G148 which binds to the…”
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Large-scale purification of the chromosomal beta-lactamase from Enterobacter cloacae P99 by Goward, C R, Stevens, G B, Hammond, P M, Scawen, M D

“…Homogeneous beta-lactamase (beta-lactam hydrolase, E.C. 3.5.2.6) from Enterobacter cloacae P99, an enzyme that has an important function in antibiotic…”
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A Single Amino Acid Mutation Enhances the Thermal Stability of Escherichia coli Malate Dehydrogenase by Goward, Christopher R., Miller, Julie, Nicholls, David J., Irons, Laurence I., Scawen, Michael D., O'brien, Ronan, Chowdhry, Babur Z.

“…The stability of wild‐type Escherichia coli malate dehydrogenase was compared with a mutant form of the enzyme with the amino acid residue at position 102…”
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Substitution of the amino acid at position 102 with polar and aromatic residues influences substrate specificity of lactate dehydrogenase by Nicholls, D J, Davey, M, Jones, S E, Miller, J, Holbrook, J J, Clarke, A R, Scawen, M D, Atkinson, T, Goward, C R

“…The Gln residue at amino acid position 102 of Bacillus stearothermophilus lactate dehydrogenase was replaced with Ser, Thr, Tyr, or Phe to investigate the…”
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