A rapid and reliable method for estimating microalgal biomass using a moisture analyser
A rapid and efficient estimation of microalgal biomass is very important to assess the growth performance of algal strains used for the production of biofuels and other high-value products. Areal and volumetric productivities of biomass are considered important in algal growth studies. A fast and re...
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Published in: | Journal of applied phycology Vol. 28; no. 3; pp. 1725 - 1734 |
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Main Authors: | , , , , , , |
Format: | Journal Article |
Language: | English |
Published: |
Dordrecht
Springer Netherlands
01-06-2016
Springer Nature B.V |
Subjects: | |
Online Access: | Get full text |
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Summary: | A rapid and efficient estimation of microalgal biomass is very important to assess the growth performance of algal strains used for the production of biofuels and other high-value products. Areal and volumetric productivities of biomass are considered important in algal growth studies. A fast and reliable method for the measurement of microalgal biomass using a moisture analyser was standardised and validated in this study using different microalgal strains, viz.,
Botryococcus braunii
,
Chlamydomonas
sp.,
Chlorella
sp.,
Arthrospira
sp. and
Chaetoceros
sp. Conventional oven-drying method was compared with moisture analyser method for analysis time, accuracy and repeatability. The average time required for analysis using a moisture analyser was 22:0, 12:38, 9:03 and 7:08 (min:s), respectively, at 60, 80, 100 and 120 °C for
B. braunii
. For the algal cultures with 0.1–1.2 g L
−1
cell concentration, 10-mL culture volume was sufficient to get reliable dry weight using moisture analyser. To measure large number of samples, a modified method was developed which required pre-drying of samples in hot air oven at 80 °C for 15 min prior to dry weight measurement in a moisture analyser at 100 °C. The drying time in the moisture analyser varied based on the cell morphology. Unicellular
Chlorella
sp. with highest cell concentration of 1.2 g L
−1
required less than 10 min of drying time, whereas the filamentous
Arthrospira
sp. with cell concentration of 1.1 g L
−1
required about 15 min. Time required for the dry weight analysis of 100 samples using modified moisture analyser method was 281:40 (min:s), and the total power consumption was 2.45 kWh, which was significantly lower than the analysis done using conventional oven-drying method. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0921-8971 1573-5176 |
DOI: | 10.1007/s10811-015-0731-1 |