Search Results - "Gouvêa, Iuri E."

Substrate specificity studies of the cysteine peptidases falcipain-2 and falcipain-3 from Plasmodium falciparum and demonstration of their kininogenase activity by Cotrin, Simone S., Gouvêa, Iuri E., Melo, Pollyana M.S., Bagnaresi, Piero, Assis, Diego M., Araújo, Mariana S., Juliano, Maria Aparecida, Gazarini, Marcos L., Rosenthal, Philip J., Juliano, Luiz, Carmona, Adriana K.

“…Plasmodial cysteine proteases falcipain-2 and falcipain-3 hydrolyze the fluorescent peptide Abz-MISLMKRPPGFSPFRSSRI-NH2 that corresponds to Met375 to Ile393…”
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Enzyme specificity and effects of gyroxin, a serine protease from the venom of the South American rattlesnake Crotalus durissus terrificus, on protease-activated receptors by Yonamine, Camila M., Kondo, Marcia Y., Nering, Marcela B., Gouvêa, Iuri E., Okamoto, Débora, Andrade, Douglas, da Silva, José Alberto A., Prieto da Silva, Álvaro R.B., Yamane, Tetsuo, Juliano, Maria A., Juliano, Luiz, Lapa, Antônio J., Hayashi, Mirian A.F., Lima-Landman, Maria Teresa R.

“…Gyroxin is a serine protease displaying a thrombin-like activity found in the venom of the South American rattlesnake Crotalus durissus terrificus. Typically,…”
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Irreversible inhibition of human cathepsins B, L, S and K by hypervalent tellurium compounds by Cunha, Rodrigo L O R, Gouvêa, Iuri E, Feitosa, Geovana P V, Alves, Márcio F M, Brömme, Dieter, Comasseto, João V, Tersariol, Ivarne L S, Juliano, Luiz

“…The inhibition of human cysteine cathepsins B, L, S and K was evaluated by a set of hypervalent tellurium compounds (telluranes) comprising both organic and…”
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A glimpse on biological activities of tellurium compounds by Cunha, Rodrigo L. O. R.(UNIFESP Departamento de Ciências Exatas e da Terra ,UNIFESP Departamento de Biofísica), Gouvea, Iuri E.(UNIFESP Departamento de Biofísica), Juliano, Luiz(UNIFESP Departamento de Biofísica)

“…Tellurium is a rare element which has been regarded as a toxic, non-essential trace element and its biological role is not clearly established to date. Besides…”
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A study of human furin specificity using synthetic peptides derived from natural substrates, and effects of potassium ions by Izidoro, Mario A., Gouvea, Iuri E., Santos, Jorge A.N., Assis, Diego M., Oliveira, Vitor, Judice, Wagner A.S., Juliano, Maria A., Lindberg, Iris, Juliano, Luiz

“…We explored furin substrate requirements in addition to the motif R-X-K/R-R using synthetic fluorescent resonance energy transfer (FRET) decapeptides. These…”
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1,2-Propanediol Dehydration in Roseburia inulinivorans: STRUCTURAL BASIS FOR SUBSTRATE AND ENANTIOMER SELECTIVITY by LaMattina, Joseph W, Keul, Nicholas D, Reitzer, Pierre, Kapoor, Suraj, Galzerani, Felipe, Koch, Daniel J, Gouvea, Iuri E, Lanzilotta, William N

“…Glycyl radical enzymes (GREs) represent a diverse superfamily of enzymes that utilize a radical mechanism to catalyze difficult, but often essential, chemical…”
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Salt effect on substrate specificity of a subtilisin-like halophilic protease by Okamoto, Debora N, Kondo, Marcia Y, Hiraga, Kazumi, Juliano, Maria A, Juliano, Luiz, Gouvea, Iuri E, Oda, Kohei

“…Enzyme-substrate interaction under the presence of high concentration of salts is of great interest for biotechnology applications and basic enzymology. In our…”
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Kosmotropic Salt Activation and Substrate Specificity of Poliovirus Protease 3C by Gouvea, Iuri E, Judice, Wagner A. S, Cezari, Maria H. S, Juliano, Maria A, Juhász, Tünde, Szeltner, Zoltán, Polgár, László, Juliano, Luiz

“…Picornaviruses produce a large polyprotein, which is cleaved by virally encoded cysteine peptidases, picornain-2A and -3C. Picornain-3C has characteristics of…”
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Functional roles of C-terminal extension (CTE) of salt-dependent peptidase activity of the Natrialba magadii extracellular protease (NEP) by Marem, Alyne, Okamoto, Debora N., Oliveira, Lilian C.G., Ruiz, Diego M., Paggi, Roberto A., Kondo, Marcia Y., Gouvea, Iuri E., Juliano, Maria A., de Castro, Rosana E., Juliano, Luiz, Icimoto, Marcelo Y.

“…Nep (Natrialba magadii extracellular protease) is a halolysin-like peptidase secreted by the haloalkaliphilic archaeon Natrialba magadii. Many extracellular…”
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Analysis of catalytic properties of tripeptidyl peptidase I (TTP-I), a serine carboxyl lysosomal protease, and its detection in tissue extracts using selective FRET peptide substrate by Kondo, Marcia Y., Gouvea, Iuri E., Okamoto, Débora N., Santos, Jorge A.N., Souccar, Caden, Oda, Kohei, Juliano, Luiz, Juliano, Maria A.

“…•NH2-RWFFIQ-EDDnp is efficient FRET substrate for tripeptidyl peptidase I (TPP-I).•S4 subsite of TPP-I allows electrostatic interaction of with substrate…”
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Internally quenched fluorescent peptide libraries with randomized sequences designed to detect endopeptidases by Oliveira, Lilian C.G., Silva, Vinícius O., Okamoto, Debora N., Kondo, Marcia Y., Santos, Saara M.B., Hirata, Isaura Y., Vallim, Marcelo A., Pascon, Renata C., Gouvea, Iuri E., Juliano, Maria A., Juliano, Luiz

“…Identification of synthetic peptide substrates for novel peptidases is an essential step for their study. With this purpose we synthesized fluorescence…”
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P-I class metalloproteinase from Bothrops moojeni venom is a post-proline cleaving peptidase with kininogenase activity: Insights into substrate selectivity and kinetic behavior by Okamoto, Débora N., Kondo, Marcia Y., Oliveira, Lilian C.G., Honorato, Rodrigo V., Zanphorlin, Leticia M., Coronado, Monika A., Araújo, Mariana S., da Motta, Guacyara, Veronez, Camila L., Andrade, Sheila S., Oliveira, Paulo S.L., Arni, Raghuvir K., Cintra, Adelia C.O., Sampaio, Suely V., Juliano, Maria A., Juliano, Luiz, Murakami, Mário T., Gouvea, Iuri E.

“…Snake venom metalloproteinases (SVMPs) belonging to P-I class are able to hydrolyze extracellular matrix proteins and coagulation factors triggering local and…”
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Studies on the Catalytic Mechanism of a Glutamic Peptidase by Kondo, Márcia Y., Okamoto, Débora N., Santos, Jorge A.N., Juliano, Maria A., Oda, Kohei, Pillai, Bindu, James, Michael N.G., Juliano, Luiz, Gouvea, Iuri E.

“…Scytalidoglutamic peptidase (SGP) is the prototype of fungal glutamic peptidases that are characteristically pepstatin insensitive. These enzymes have a unique…”
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Mycoplasma hyopneumoniae in vitro peptidase activities: Identification and cleavage of kallikrein-kinin system-like substrates by Moitinho-Silva, Lucas, Kondo, Marcia Y., Oliveira, Lilian C.G., Okamoto, Debora N., Paes, Jéssica A., Machado, Mauricio F.M., Veronez, Camila L., Motta, Guacyara, Andrade, Sheila S., Juliano, Maria A., Ferreira, Henrique B., Juliano, Luiz, Gouvea, Iuri E.

“…Bacterial proteases are important for metabolic processes and pathogenesis in host organisms. The bacterial swine pathogen Mycoplasma hyopneumoniae has 15…”
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Analysis of peptidase activities of a cathepsin B-like (TcoCBc1) from Trypanosoma congolense by Oliveira, Lilian C.G., Okamoto, Débora N., Oliveira, Juliana R., Kondo, Marcia Y., Gouvea, Iuri E., Biteau, Nicolas, Baltz, Theo, Murakami, Mário T., Juliano, Luiz, Juliano, Maria A.

“…The substrate specificity of TcoCBc1 was evaluated using two internally quenched fluorescent peptide libraries with randomized sequences designed to detect…”
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Yellow fever virus NS2B/NS3 protease: Hydrolytic Properties and Substrate Specificity by Kondo, Marcia Y., Oliveira, Lilian C.G., Okamoto, Debora N., de Araujo, Marina R.T., Duarte dos Santos, Claudia N., Juliano, Maria A., Juliano, Luiz, Gouvea, Iuri E.

“…► YFV contains the same substrate motif in all processing sites. ► The kcat/km varied by more than two orders of magnitude among then. ► Obtained data parallel…”
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Correlation between catalysis and tertiary structure arrangement in an archaeal halophilic subtilase by Souza, Tatiana A.C.B., Okamoto, Débora N., Ruiz, Diego M., Oliveira, Lilian C.G., Kondo, Márcia Y., Tersario, Ivarne L.S., Juliano, Luiz, De Castro, Rosana E., Gouvea, Iuri E., Murakami, Mário T.

“…Nep (Natrialba magadii extracellular protease) is a halolysin-like peptidase secreted by the haloalkaliphilic archaeon N. magadii that exhibits optimal…”
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Increase of SARS-CoV 3CL peptidase activity due to macromolecular crowding effects in the milieu composition by Okamoto, Debora N, Oliveira, Lilian C G, Kondo, Marcia Y, Cezari, Maria H S, Szeltner, Zoltán, Juhász, Tünde, Juliano, Maria A, Polgár, László, Juliano, Luiz, Gouvea, Iuri E

“…The 3C-like peptidase of the severe acute respiratory syndrome virus (SARS-CoV) is strictly required for viral replication, thus being a potential target for…”
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Poliovirus 3C proteinase inhibition by organotelluranes by Gouvea, Iuri E, Santos, Jorge A N, Burlandy, Fernanda M, Tersariol, Ivarne L S, da Silva, Edson E, Juliano, Maria A, Juliano, Luiz, Cunha, Rodrigo L O R

“…The 3C proteinase, essential for human poliovirus (PV) replication, has unique characteristics as its three-dimensional structure resembles chymotrypsin, but…”
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Biochemical characterization of a cysteine proteinase from Bauhinia forficata leaves and its kininogenase activity by Andrade, Sheila S., Silva-Lucca, Rosemeire A., Santana, Lucimeire A., Gouvea, Iuri E., Juliano, Maria A., Carmona, Adriana K., Araújo, Mariana S., Sampaio, Misako U., Oliva, Maria Luiza V.

“…In this work, the proteinase activity detect in the acetone precipitate (80%, v/v) of B. forficata leaves, trivially known as cow paw, and popularly used in…”
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