Multipotent progenitor cells in gingival connective tissue

Multipotent progenitor cells in gingival connective tissue

The gum has an exceptional capacity for healing. To examine the basis for this property and explore the potential of conferring it to organs with inferior healing capacity, we sought the presence of progenitor cells in gingival connective tissue. Colony-forming units of fibroblast-enriched cells fro...

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Bibliographic Details
Published in:Tissue engineering. Part A Vol. 16; no. 9; p. 2891
Main Authors: Fournier, Benjamin P J, Ferre, François C, Couty, Ludovic, Lataillade, Jean-Jacques, Gourven, Murielle, Naveau, Adrien, Coulomb, Bernard, Lafont, Antoine, Gogly, Bruno
Format: Journal Article
Language:English
Published: United States 01-09-2010
Subjects:
Adipocytes - cytology
Adult
Aged
Blotting, Western
Calcium - metabolism
Cell Differentiation - genetics
Cell Differentiation - physiology
Cells, Cultured
Chondrocytes - cytology
Female
Fibroblasts - cytology
Flow Cytometry
Gingiva - cytology
Humans
Immunohistochemistry
Male
Middle Aged
Multipotent Stem Cells - cytology
Multipotent Stem Cells - metabolism
Osteoblasts - cytology
Young Adult
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Summary:The gum has an exceptional capacity for healing. To examine the basis for this property and explore the potential of conferring it to organs with inferior healing capacity, we sought the presence of progenitor cells in gingival connective tissue. Colony-forming units of fibroblast-enriched cells from gingival fibroblast cultures were assessed for expression of membrane markers of mesenchymal stem cells; capacity to differentiate into osteoblasts, chondroblasts, and adipocytes; and engraftment efficiency after in vivo transfer. On the basis of their ability to differentiate into several lineages, proliferate from single cells, induce calcium deposits, and secrete collagen in vivo after transfer on hydroxyapatite carriers, we suggest that this population represents gingival multipotent progenitor cells. The discovery of progenitor cells in gingival connective tissue may help improve our understanding of how the wounded gum is capable of almost perfect healing and opens the prospect of cellular therapy for wound healing using readily available cells at limited risk to the patient.
ISSN:1937-335X
DOI:10.1089/ten.tea.2009.0796