Search Results - "Goulding, Joelle"

Application of BRET to monitor ligand binding to GPCRs by Stoddart, Leigh A, Johnstone, Elizabeth K M, Wheal, Amanda J, Goulding, Joëlle, Robers, Matthew B, Machleidt, Thomas, Wood, Keith V, Hill, Stephen J, Pfleger, Kevin D G

“…Bioluminescence resonance energy transfer, from the substrate of a luciferase fused with a G protein–coupled receptor to a fluorescent dye covalently linked to…”
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Advances in the application of fluorescence correlation spectroscopy to study detergent purified and encapsulated membrane proteins by Stoddart, Leigh A, Goulding, Joëlle, Briddon, Stephen J

“…Fluorescence correlation spectroscopy (FCS) is a quantitative spectroscopy technique which could potentially increase throughput and sensitivity of screening…”
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Ligand-Directed Labeling of the Adenosine A1 Receptor in Living Cells by Comeo, Eleonora, Goulding, Joëlle, Lin, Chia-Yang, Groenen, Marleen, Woolard, Jeanette, Kindon, Nicholas D., Harwood, Clare R., Platt, Simon, Briddon, Stephen J., Kilpatrick, Laura E., Scammells, Peter J., Hill, Stephen J., Kellam, Barrie

“…The study of protein function and dynamics in their native cellular environment is essential for progressing fundamental science. To overcome the requirement…”
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Small-Molecule Fluorescent Ligands for the CXCR4 Chemokine Receptor by Dekkers, Sebastian, Caspar, Birgit, Goulding, Joëlle, Kindon, Nicholas D., Kilpatrick, Laura E., Stoddart, Leigh A., Briddon, Stephen J., Kellam, Barrie, Hill, Stephen J., Stocks, Michael J.

“…The C–X–C chemokine receptor type 4, or CXCR4, is a chemokine receptor found to promote cancer progression and metastasis of various cancer cell types. To…”
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A high-affinity, cis-on photoswitchable beta blocker to optically control β2-adrenergic receptors in vitro and in vivo by Shi, Shuang, Zheng, Yang, Goulding, Joëlle, Marri, Silvia, Lucarini, Laura, Konecny, Benjamin, Sgambellone, Silvia, Villano, Serafina, Bosma, Reggie, Wijtmans, Maikel, Briddon, Stephen J., Zarzycka, Barbara A., Vischer, Henry F., Leurs, Rob

“…[Display omitted] This study introduces (S)-Opto-prop-2, a second-generation photoswitchable ligand designed for precise modulation of β2-adrenoceptor (β2AR)…”
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Detection of genome‐edited and endogenously expressed G protein‐coupled receptors by Soave, Mark, Stoddart, Leigh A., White, Carl W., Kilpatrick, Laura E., Goulding, Joëlle, Briddon, Stephen J., Hill, Stephen J.

“…G protein‐coupled receptors (GPCRs) are the largest family of membrane receptors and major targets for FDA‐approved drugs. The ability to quantify GPCR…”
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Acylation of the Incretin Peptide Exendin-4 Directly Impacts Glucagon-Like Peptide-1 Receptor Signaling and Trafficking by Lucey, Maria, Ashik, Tanyel, Marzook, Amaara, Wang, Yifan, Goulding, Joëlle, Oishi, Atsuro, Broichhagen, Johannes, Hodson, David J., Minnion, James, Elani, Yuval, Jockers, Ralf, Briddon, Stephen J., Bloom, Stephen R., Tomas, Alejandra, Jones, Ben

“…The glucagon-like peptide-1 receptor (GLP-1R) is a class B G protein- coupled receptor and mainstay therapeutic target for the treatment of type 2 diabetes and…”
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Application of Fluorescent Purinoceptor Antagonists for Bioluminescence Resonance Energy Transfer Assays and Fluorescent Microscopy by Soave, Mark, Goulding, Joëlle, Markus, Robert, Hill, Stephen J, Stoddart, Leigh A

“…Fluorescent antagonists offer the ability to interrogate G protein-coupled receptor pharmacology. With resonance energy transfer techniques, fluorescent…”
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A novel and selective fluorescent ligand for the study of adenosine A2B receptors by Patera, Foteini, Mistry, Sarah J., Kindon, Nicholas D., Comeo, Eleonora, Goulding, Joelle, Kellam, Barrie, Kilpatrick, Laura E., Franks, Hester, Hill, Stephen J.

“…Fluorescent ligands have proved to be powerful tools in the study of G protein‐coupled receptors in living cells. Here we have characterized a new fluorescent…”
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CRISPR/Cas9-mediated generation and analysis of N terminus polymorphic models of β2AR in isogenic hPSC-derived cardiomyocytes by Kondrashov, Alexander, Mohd Yusof, Nurul A.N., Hasan, Alveera, Goulding, Joëlle, Kodagoda, Thusharika, Hoang, Duc M., Vo, Nguyen T.N., Melarangi, Tony, Dolatshad, Nazanin, Gorelik, Julia, Hill, Stephen J., Harding, Sian E., Denning, Chris

“…During normal- and patho-physiological situations, the behavior of the beta2-adrenoreceptor (β2AR) is influenced by polymorphic variants. The functional impact…”
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Probing expression of E-selectin using CRISPR-Cas9-mediated tagging with HiBiT in human endothelial cells by Ogrodzinski, Lydia, Platt, Simon, Goulding, Joelle, Alexander, Cameron, Farr, Tracy D., Woolard, Jeanette, Hill, Stephen J., Kilpatrick, Laura E.

“…E-selectin is expressed on endothelial cells in response to inflammatory cytokines and mediates leukocyte rolling and extravasation. However, studies have been…”
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Characterisation of endogenous A2A and A2B receptor-mediated cyclic AMP responses in HEK 293 cells using the GloSensor™ biosensor: Evidence for an allosteric mechanism of action for the A2B-selective antagonist PSB 603 by Goulding, Joelle, May, Lauren T., Hill, Stephen J.

“…[Display omitted] Endogenous adenosine A2B receptors (A2BAR) mediate cAMP accumulation in HEK 293 cells. Here we have used a biosensor to investigate the…”
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Single molecule binding of a ligand to a G-protein-coupled receptor in real time using fluorescence correlation spectroscopy, rendered possible by nano-encapsulation in styrene maleic acid lipid particles by Grime, Rachael L, Goulding, Joelle, Uddin, Romez, Stoddart, Leigh A, Hill, Stephen J, Poyner, David R, Briddon, Stephen J, Wheatley, Mark

“…The fundamental importance of membrane proteins in cellular processes has driven a marked increase in the use of membrane mimetic approaches for studying and…”
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Subtype selective fluorescent ligands based on ICI 118,551 to study the human β2‐adrenoceptor in CRISPR/Cas9 genome‐edited HEK293T cells at low expression levels by Goulding, Joëlle, Mistry, Sarah J., Soave, Mark, Woolard, Jeanette, Briddon, Stephen J., White, Carl W., Kellam, Barrie, Hill, Stephen J.

“…Fluorescent ligand technologies have proved to be powerful tools to improve our understanding of ligand‐receptor interactions. Here we have characterized a…”
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Probing the binding of interleukin-23 to individual receptor components and the IL-23 heteromeric receptor complex in living cells using NanoBRET by Lay, Charles S, Bridges, Angela, Goulding, Joelle, Briddon, Stephen J, Soloviev, Zoja, Craggs, Peter D, Hill, Stephen J

“…Interleukin-23 (IL-23) is a pro-inflammatory cytokine involved in the host defense against pathogens but is also implicated in the development of several…”
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The tetraspanin Tspan15 is an essential subunit of an ADAM10 scissor complex by Koo, Chek Ziu, Harrison, Neale, Noy, Peter J., Szyroka, Justyna, Matthews, Alexandra L., Hsia, Hung-En, Müller, Stephan A., Tüshaus, Johanna, Goulding, Joelle, Willis, Katie, Apicella, Clara, Cragoe, Bethany, Davis, Edward, Keles, Murat, Malinova, Antonia, McFarlane, Thomas A., Morrison, Philip R., Nguyen, Hanh T.H., Sykes, Michael C., Ahmed, Haroon, Di Maio, Alessandro, Seipold, Lisa, Saftig, Paul, Cull, Eleanor, Pliotas, Christos, Rubinstein, Eric, Poulter, Natalie S., Briddon, Stephen J., Holliday, Nicholas D., Lichtenthaler, Stefan F., Tomlinson, Michael G.

“…A disintegrin and metalloprotease 10 (ADAM10) is a transmembrane protein essential for embryonic development, and its dysregulation underlies disorders such as…”
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A novel and selective fluorescent ligand for the study of adenosine A 2B receptors by Patera, Foteini, Mistry, Sarah J, Kindon, Nicholas D, Comeo, Eleonora, Goulding, Joelle, Kellam, Barrie, Kilpatrick, Laura E, Franks, Hester, Hill, Stephen J

“…Fluorescent ligands have proved to be powerful tools in the study of G protein-coupled receptors in living cells. Here we have characterized a new fluorescent…”
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Characterisation of endogenous A 2A and A 2B receptor-mediated cyclic AMP responses in HEK 293 cells using the GloSensor™ biosensor: Evidence for an allosteric mechanism of action for the A 2B -selective antagonist PSB 603 by Goulding, Joelle, May, Lauren T, Hill, Stephen J

“…Endogenous adenosine A receptors (A AR) mediate cAMP accumulation in HEK 293 cells. Here we have used a biosensor to investigate the mechanism of action of the…”
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Plasma membrane preassociation drives β-arrestin coupling to receptors and activation by Grimes, Jak, Koszegi, Zsombor, Lanoiselée, Yann, Miljus, Tamara, O’Brien, Shannon L., Stepniewski, Tomasz M., Medel-Lacruz, Brian, Baidya, Mithu, Makarova, Maria, Mistry, Ravi, Goulding, Joëlle, Drube, Julia, Hoffmann, Carsten, Owen, Dylan M., Shukla, Arun K., Selent, Jana, Hill, Stephen J., Calebiro, Davide

“…β-arrestin plays a key role in G protein-coupled receptor (GPCR) signaling and desensitization. Despite recent structural advances, the mechanisms that govern…”
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Ligand-Directed Labeling of the Adenosine A 1 Receptor in Living Cells by Comeo, Eleonora, Goulding, Joëlle, Lin, Chia-Yang, Groenen, Marleen, Woolard, Jeanette, Kindon, Nicholas D, Harwood, Clare R, Platt, Simon, Briddon, Stephen J, Kilpatrick, Laura E, Scammells, Peter J, Hill, Stephen J, Kellam, Barrie

“…The study of protein function and dynamics in their native cellular environment is essential for progressing fundamental science. To overcome the requirement…”
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