Search Results - "Gouka, R.J"

Bitter Taste Receptor Activation by Flavonoids and Isoflavonoids: Modeled Structural Requirements for Activation of hTAS2R14 and hTAS2R39 by Roland, Wibke S. U, van Buren, Leo, Gruppen, Harry, Driesse, Marianne, Gouka, Robin J, Smit, Gerrit, Vincken, Jean-Paul

“…Many flavonoids and isoflavonoids have an undesirable bitter taste, which hampers their use as food bioactives. The aim of this study was to investigate the…”
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Evaluation of the Bitter-Masking Potential of Food Proteins for EGCG by a Cell-Based Human Bitter Taste Receptor Assay and Binding Studies by Bohin, Maxime C, Roland, Wibke S. U, Gruppen, Harry, Gouka, Robin J, van der Hijden, Harry T. W. M, Dekker, Peter, Smit, Gerrit, Vincken, Jean-Paul

“…Epigallocatechin gallate (EGCG) has been ascribed to several health benefits, but its bitter taste influences the liking of products with high concentrations…”
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Snooker structure-based pharmacophore model explains differences in agonist and blocker binding to bitter receptor hTAS2R39 by Roland, Wibke S U, Sanders, Marijn P A, van Buren, Leo, Gouka, Robin J, Gruppen, Harry, Vincken, Jean-Paul, Ritschel, Tina

“…The human bitter taste receptor hTAS2R39 can be activated by many dietary (iso)flavonoids. Furthermore, hTAS2R39 activity can be blocked by…”
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Soy Isoflavones and Other Isoflavonoids Activate the Human Bitter Taste Receptors hTAS2R14 and hTAS2R39 by Roland, Wibke S.U, Vincken, Jean-Paul, Gouka, Robin J, van Buren, Leo, Gruppen, Harry, Smit, Gerrit

“…The aim of this study was to identify the bitter receptor(s) that recognize the bitter taste of the soy isoflavone genistein. Screening of all 25 human bitter…”
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Kinetics of mRNA and protein synthesis of genes controlled by the 1,4-beta-endoxylanase A promoter in controlled fermentations of Aspergillus awamori by Gouka, R.J. (Unilever Research Laboratorium, Vlaardingen, The Netherlands.), Stam, H, Fellinger, A.J, Muijsenberg, R.J.G.T, Wijngaard, A.J. van de, Punt, P.J, Musters, W, Hondel, C.A.M.J.J. van den

“…In this study, induction and repression kinetics of the expression of the Aspergillus awamori 1,4-beta-endoxylanase A (exlA) gene under defined physiological…”
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Efficient production of secreted proteins by Aspergillus: progress, limitations and prospects by Gouka, R.J, Punt, P.J, Hondel, C.A.M.J.J. van den

“…Filamentous fungi are widely used for the production of homologous and heterologous proteins but, compared to homologous proteins, the levels of production of…”
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Expression and production of llama variable heavy-chain antibody fragments (VHHs) by Aspergillus awamori by Joosten, Vivi, Gouka, Robin J, van den Hondel, Cees A. M. J. J, Verrips, C. Theo, Lokman, B. Christien

“…We report the expression and production of llama variable heavy-chain antibody fragments (VHHs) by Aspergillus awamori. Fragments encoding VHHs were cloned in…”
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Transformation of Aspergillus awamori by Agrobacterium tumefaciens-mediated homologous recombination by Gouka, R.J, Gerk, C, Hooykaas, P.J.J, Bundock, P, Musters, W, Verrips, C.T, Groot, M.J.A. de

“…Agrobacterium tumefaciens is known to transfer part of its tumor-inducing (Ti) plasmid to the filamentous fungus Aspergillus awamori by illegitimate…”
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6-Methoxyflavanones as Bitter Taste Receptor Blockers by Roland, W.S.U, Gouka, R.J, Gruppen, H, Driesse, M, Buren, L., van, Smit, G, Vincken, J.P

“…Many (dietary) bitter compounds, e.g. flavonoids, activate bitter receptor hTAS2R39 in cell-based assays. Several flavonoids, amongst which some flavanones,…”
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Glucoamylase gene fusions alleviate limitations for protein production in Aspergillus awamori at the transcriptional and (post) translational levels by GOUKA, R. J, PUNT, P. J, VAN DEN HONDEL, C. A. M. J. J

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Cloning, characterization and overexpression of the phytase-encoding gene (phyA) of Aspergillus niger by van Hartingsveldt, W, van Zeijl, C M, Harteveld, G M, Gouka, R J, Suykerbuyk, M E, Luiten, R G, van Paridon, P A, Selten, G C, Veenstra, A E, van Gorcom, R F

“…Phytase catalyzes the hydrolysis of phytate (myo-inositol hexakisphosphate) to myo-inositol and inorganic phosphate. A gene (phyA) of Aspergillus niger…”
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expression system based on the promoter region of the Aspergillus awamori 1,4-beta-endoxylanase A gene by Gouka, R.J, Hessing, J.G.M, Punt, P.J, Stam, H, Musters, W, Hondel, C.A.M.J.J. van den

“…A new, highly inducible fungal promoter derived from the Aspergillus awamori 1,4-beta-endoxylanase A (exlA) gene is described. Induction analysis, carried out…”
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A novel strategy for the isolation of defined pyrG mutants and the development of a site specific integration system for Aspergillus awamori by Gouka, R.J, Hessing, J.G.M, Stam, H, Musters, W, Hondel, C.A.M.J.J. van den (TNO Nutrition and Food Research Inst., Rijswijk (Netherlands). Dept. of Molecular Genetics and Gene Technology)

“…A homologous gene transfer system for Aspergillus awamori for site-specific integration is described, based on two components. First, a defined A. awamori pyrG…”
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Cloning of the nitrate-nitrite reductase gene cluster of Penicillium chrysogenum and use of the niaD gene as a homologous selection marker by Gouka, R J, van Hartingsveldt, W, Bovenberg, R A, van den Hondel, C A, van Gorcom, R F

“…A new homologous transformation system for the filamentous fungus Penicillium chrysogenum is described. The system is based on complementation of niaD mutants…”
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Development of a new transformant selection system for Penicillium chrysogenum: isolation and characterization of the P. chrysogenum acetyl-coenzyme A synthetase gene (facA) and its use as a homologous selection marker by Gouka, R.J, Hartingsveldt, W. van, Bovenberg, R.A.L, Zeijl, C.M.J. van, Hondel, C.A.M.J.J. van den, Gorcom, R.F.M. van

“…A new transformation system for the filamentous fungus Penicillium chrysogenum is described, based on the use of the homologous acetyl-coenzyme A synthetase…”
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