Search Results - "Gouka, R J"

Efficient production of secreted proteins by Aspergillus: progress, limitations and prospects by Gouka, R.J, Punt, P.J, Hondel, C.A.M.J.J. van den

“…Filamentous fungi are widely used for the production of homologous and heterologous proteins but, compared to homologous proteins, the levels of production of…”
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Transformation of Aspergillus awamori by Agrobacterium tumefaciens-mediated homologous recombination by Gouka, R.J, Gerk, C, Hooykaas, P.J.J, Bundock, P, Musters, W, Verrips, C.T, Groot, M.J.A. de

“…Agrobacterium tumefaciens is known to transfer part of its tumor-inducing (Ti) plasmid to the filamentous fungus Aspergillus awamori by illegitimate…”
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Analysis of heterologous protein production in defined recombinant Aspergillus awamori strains by GOUKA, R. J, PUNT, P. J, HESSING, J. G. M, VAN DEN HONDEL, C. A. M. J. J

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Glucoamylase gene fusions alleviate limitations for protein production in Aspergillus awamori at the transcriptional and (post) translational levels by GOUKA, R. J, PUNT, P. J, VAN DEN HONDEL, C. A. M. J. J

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The effect of pre- and pro-sequences and multicopy integration on heterologous expression of the Fusarium solani pisi cutinase gene in Aspergillus awamori by VAN GEMEREN, I. A, BEIJERSBERGEN, A, MUSTERS, W, GOUKA, R. J, VAN DEN HONDEL, C. A. M. J. J, VERRIPS, C. T

“…A synthetic derivative of the cutinase cDNA of Fusarium solani pisi was expressed in Aspergillus awamori using the A. awamori endoxylanase II (exlA) promoter…”
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expression system based on the promoter region of the Aspergillus awamori 1,4-beta-endoxylanase A gene by Gouka, R.J, Hessing, J.G.M, Punt, P.J, Stam, H, Musters, W, Hondel, C.A.M.J.J. van den

“…A new, highly inducible fungal promoter derived from the Aspergillus awamori 1,4-beta-endoxylanase A (exlA) gene is described. Induction analysis, carried out…”
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Cloning, characterization and overexpression of the phytase-encoding gene (phyA) of Aspergillus niger by van Hartingsveldt, W, van Zeijl, C M, Harteveld, G M, Gouka, R J, Suykerbuyk, M E, Luiten, R G, van Paridon, P A, Selten, G C, Veenstra, A E, van Gorcom, R F

“…Phytase catalyzes the hydrolysis of phytate (myo-inositol hexakisphosphate) to myo-inositol and inorganic phosphate. A gene (phyA) of Aspergillus niger…”
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Kinetics of mRNA and protein synthesis of genes controlled by the 1,4-beta-endoxylanase A promoter in controlled fermentations of Aspergillus awamori by Gouka, R.J. (Unilever Research Laboratorium, Vlaardingen, The Netherlands.), Stam, H, Fellinger, A.J, Muijsenberg, R.J.G.T, Wijngaard, A.J. van de, Punt, P.J, Musters, W, Hondel, C.A.M.J.J. van den

“…In this study, induction and repression kinetics of the expression of the Aspergillus awamori 1,4-beta-endoxylanase A (exlA) gene under defined physiological…”
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A novel strategy for the isolation of defined pyrG mutants and the development of a site specific integration system for Aspergillus awamori by Gouka, R.J, Hessing, J.G.M, Stam, H, Musters, W, Hondel, C.A.M.J.J. van den (TNO Nutrition and Food Research Inst., Rijswijk (Netherlands). Dept. of Molecular Genetics and Gene Technology)

“…A homologous gene transfer system for Aspergillus awamori for site-specific integration is described, based on two components. First, a defined A. awamori pyrG…”
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Cloning of a phenol oxidase gene from Acremonium murorum and its expression in Aspergillus awamori by GOUKA, Robin J, VAN DER HEIDEN, Monique, SWARTHOFF, Ton, VERRIPS, C. Theo

“…Fungal multicopper oxidases have many potential industrial applications, since they perform reactions under mild conditions. We isolated a phenol oxidase from…”
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Efficient production of Arthromyces ramosus peroxidase by Aspergillus awamori by Lokman, B.Christien, Joosten, Vivi, Hovenkamp, Jacqueline, Gouka, Robin J., Verrips, C.Theo, van den Hondel, Cees A.M.J.J.

“…The heterologous production of Arthromyces ramosus peroxidase (ARP) was analysed in the filamentous fungus Aspergillus awamori under control of the inducible…”
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Development of a new transformant selection system for Penicillium chrysogenum: isolation and characterization of the P. chrysogenum acetyl-coenzyme A synthetase gene (facA) and its use as a homologous selection marker by Gouka, R.J, Hartingsveldt, W. van, Bovenberg, R.A.L, Zeijl, C.M.J. van, Hondel, C.A.M.J.J. van den, Gorcom, R.F.M. van

“…A new transformation system for the filamentous fungus Penicillium chrysogenum is described, based on the use of the homologous acetyl-coenzyme A synthetase…”
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Cloning of the nitrate-nitrite reductase gene cluster of Penicillium chrysogenum and use of the niaD gene as a homologous selection marker by Gouka, R J, van Hartingsveldt, W, Bovenberg, R A, van den Hondel, C A, van Gorcom, R F

“…A new homologous transformation system for the filamentous fungus Penicillium chrysogenum is described. The system is based on complementation of niaD mutants…”
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Expression and production of llama variable heavy-chain antibody fragments (V(HH)s) by Aspergillus awamori by Joosten, Vivi, Gouka, Robin J, van den Hondel, Cees A M J J, Verrips, C Theo, Lokman, B Christien

“…We report the expression and production of llama variable heavy-chain antibody fragments (V(HH)s) by Aspergillus awamori. Fragments encoding V(HH)s were cloned…”
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