Search Results - "Goudar, Chetan T."

A framework for real-time glycosylation monitoring (RT-GM) in mammalian cell culture by Tharmalingam, Tharmala, Wu, Chao-Hsiang, Callahan, Susan, T. Goudar, Chetan

“…ABSTRACT Glycosylation is a critical characteristic of biotherapeutics because of its central role in in vivo efficacy. Multiple factors including medium…”
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Engineering protein glycosylation in CHO cells to be highly similar to murine host cells by Gupta, Shivani, Shah, Bhavana, Fung, Coral Shek, Chan, Pik Kay, Wakefield, Devin L, Kuhns, Scott, Goudar, Chetan T, Piret, James M

“…Since 2015 more than 34 biosimilars have been approved by the FDA. This new era of biosimilar competition has stimulated renewed technology development focused…”
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Turbulent Flow Friction Factor Calculation Using a Mathematically Exact Alternative to the Colebrook–White Equation by Sonnad, Jagadeesh R, Goudar, Chetan T

“…We present a novel, mathematically equivalent representation of the Colebrook–White equation to compute friction factor for turbulent flow in rough pipes. This…”
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From chemostats to high‐density perfusion: the progression of continuous mammalian cell cultivation by Wong, H. Edward, Chen, Chun, Le, Huong, Goudar, Chetan T.

“…Continuous cultivation of mammalian cells originated in chemostats in the 1950s and, through a series of technological advancements, has evolved into…”
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A high-resolution multi-attribute method for product characterization, process characterization, and quality control of therapeutic proteins by Guan, Xiaoyan, Eris, Tamer, Zhang, Le, Ren, Da, Ricci, Margaret S., Thiel, Tiffany, Goudar, Chetan T.

“…During the manufacturing of therapeutic proteins, Critical Quality Attributes (CQAs) have been monitored by conventional methods, such as cation exchange…”
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Evaluation of two public genome references for chinese hamster ovary cells in the context of rna‐seq based gene expression analysis by Chen, Chun, Le, Huong, Goudar, Chetan T.

“…ABSTRACT RNA‐Seq is a powerful transcriptomics tool for mammalian cell culture process development. Successful RNA‐Seq data analysis requires a high quality…”
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Upstream process intensification and continuous manufacturing by Chen, Chun, Wong, H Edward, Goudar, Chetan T

“…Manufacturing process of biopharmaceuticals usually starts from culturing recombinant protein producing cells, predominately Chinese Hamster Ovary (CHO) cells,…”
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Recent advances in the understanding of biological implications and modulation methodologies of monoclonal antibody N-linked high mannose glycans by Shi, Helen H., Goudar, Chetan T.

“…ABSTRACT With the prevalence of therapeutic monoclonal antibodies (mAbs) in the biopharmaceutical industry, the use of mammalian cell culture systems,…”
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Evaluating the impact of high Pluronic® F68 concentrations on antibody producing CHO cell lines by Tharmalingam, Tharmala, Goudar, Chetan T.

“…ABSTRACT Pluronic® F68 (P‐F68) is an important component of chemically‐defined cell culture medium because it protects cells from hydrodynamic and…”
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Elucidating the role of copper in CHO cell energy metabolism using (13)C metabolic flux analysis by Nargund, Shilpa, Qiu, Jinshu, Goudar, Chetan T

“…(13)C-metabolic flux analysis was used to understand copper deficiency-related restructuring of energy metabolism, which leads to excessive lactate production…”
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Logistic Equations Effectively Model Mammalian Cell Batch and Fed-Batch Kinetics by Logically Constraining the Fit by Goudar, Chetan T., Joeris, Klaus, Konstantinov, Konstantin B., Piret, James M.

“…A four‐parameter logistic equation was used to fit batch and fed‐batch time profiles of viable cell density in order to estimate net growth rates from the…”
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Integration of systems biology in cell line and process development for biopharmaceutical manufacturing by Chen, Chun, Le, Huong, Goudar, Chetan T.

“…•We discuss systems biology approaches in cell line and process development.•We summarize key enablers for systems biology applications in CHO cells.•We…”
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An evaluation of public genomic references for mapping RNA-Seq data from Chinese hamster ovary cells by Le, Huong, Chen, Chun, Goudar, Chetan T.

“…ABSTRACT While RNA‐Seq is increasingly used as the method of choice for transcriptome analysis of mammalian cell culture processes, no universal genomic…”
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An automated RNA-Seq analysis pipeline to identify and visualize differentially expressed genes and pathways in CHO cells by Chen, Chun, Le, Huong, Goudar, Chetan T.

“…Recent advances in RNA‐Seq based comparative transcriptomics have opened up a unique opportunity to understand the mechanisms of different phenotypes in…”
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Enabling development, manufacturing, and regulatory approval of biotherapeutics through advances in mass spectrometry by Apostol, Izydor, Bondarenko, Pavel V, Ren, Da, Semin, David J, Wu, Chao-Hsiang, Zhang, Zhongqi, Goudar, Chetan T

“…Rapid technological advances have significantly improved the capability, versatility, and robustness of mass spectrometers which has led to them playing a…”
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State-of-the-art and emerging trends in analytical approaches to pharmaceutical-product commercialization by Blue, Laura E, Guan, Xiaoyan, Joubert, Marisa K, Kuhns, Scott T, Moore, Stephanie, Semin, David J, Wikström, Mats, Wypych, Jette, Goudar, Chetan T

“…The biopharmaceutical landscape continues to evolve rapidly, and associated modality complexity and the need to improve molecular understanding require…”
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Characterization of intrinsic variability in time-series metabolomic data of cultured mammalian cells by Le, Huong, Jerums, Matthew, Goudar, Chetan T.

“…ABSTRACT In an attempt to rigorously characterize the intrinsic variability associated with Chinese Hamster Ovary (CHO) cell metabolomics studies, supernatant…”
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Accelerating attribute‐focused process and product development through the development and deployment of autonomous process analytical technology platform system by Wu, Chao‐Hsiang (Richard), Chan, Becky, Sarich, Zac, Duan, Yaokai, Chen, Janice, Song, Jiu‐Li, Berke, Mike, Miranda, Les P., Goudar, Chetan T.

“…Enabling real‐time monitoring and control of the biomanufacturing processes through product quality insights continues to be an area of focus in the…”
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Methods for Estimating the Probability of Clonality in Cell Line Development by Chen, Chun, Le, Kim, Le, Huong, Daris, Kristi, Soice, Neil, Stevens, Jennitte, Goudar, Chetan T.

“…Mammalian cell banks for biopharmaceutical production are usually derived from a single progenitor cell. Different methods to estimate the probability that the…”
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Progress curve analysis for enzyme and microbial kinetic reactions using explicit solutions based on the Lambert W function by Goudar, Chetan T., Harris, Steve K., McInerney, Michael J., Suflita, Joseph M.

“…We present a simple method for estimating kinetic parameters from progress curve analysis of biologically catalyzed reactions that reduce to forms analogous to…”
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