Gene expression analysis using oligonucleotide arrays produced by maskless photolithography

Microarrays containing 195,000 in situ synthesized oligonucleotide features have been created using a benchtop, maskless photolithographic instrument. This instrument, the Maskless Array Synthesizer (MAS), uses a digital light processor (DLP) developed by Texas Instruments. The DLP creates the patte...

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Bibliographic Details
Published in:	Genome research Vol. 12; no. 11; pp. 1749 - 1755
Main Authors:	Nuwaysir, Emile F, Huang, Wei, Albert, Thomas J, Singh, Jaz, Nuwaysir, Kate, Pitas, Alan, Richmond, Todd, Gorski, Tom, Berg, James P, Ballin, Jeff, McCormick, Mark, Norton, Jason, Pollock, Tim, Sumwalt, Terry, Butcher, Lawrence, Porter, DeAnn, Molla, Michael, Hall, Christine, Blattner, Fred, Sussman, Michael R, Wallace, Rodney L, Cerrina, Franco, Green, Roland D
Format:	Journal Article
Language:	English
Published:	United States Cold Spring Harbor Laboratory Press 01-11-2002
Subjects:	Animals DNA - biosynthesis DNA - chemistry DNA - genetics Drosophila melanogaster - genetics Gene Expression Profiling - instrumentation Gene Expression Profiling - methods Genes - genetics Genes, Insect - genetics Methods Mice Oligonucleotide Array Sequence Analysis - instrumentation Oligonucleotide Array Sequence Analysis - methods Oligonucleotide Probes - chemistry Photochemistry - instrumentation Photochemistry - methods
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Summary:	Microarrays containing 195,000 in situ synthesized oligonucleotide features have been created using a benchtop, maskless photolithographic instrument. This instrument, the Maskless Array Synthesizer (MAS), uses a digital light processor (DLP) developed by Texas Instruments. The DLP creates the patterns of UV light used in the light-directed synthesis of oligonucleotides. This digital mask eliminates the need for expensive and time-consuming chromium masks. In this report, we describe experiments in which we tested this maskless technology for DNA synthesis on glass surfaces. Parameters examined included deprotection rates, repetitive yields, and oligonucleotide length. Custom gene expression arrays were manufactured and hybridized to Drosophila melanogaster and mouse samples. Quantitative PCR was used to validate the gene expression data from the mouse arrays.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Corresponding author.
ISSN:	1088-9051 1549-5469
DOI:	10.1101/gr.362402