Vitrification of cat ovarian tissue: Does fragment size matters?

Vitrification of cat ovarian tissue: Does fragment size matters?

Contents The aim of the present study was to investigate whether or not the size of the ovarian fragment influences its resistance to cryostorage. For that purpose, ovaries were collected from 34 queens (various breeds, age 1–5 year) by routine ovariectomy, transported to the laboratory and then sec...

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Bibliographic Details
Published in:Reproduction in domestic animals Vol. 53; no. S3; pp. 125 - 132
Main Authors: Gorricho, Camila M., Tavares, Mariana R., Apparício, Maricy, Fonseca‐Alves, Carlos E., Macente, Beatrice I., Mansano, Cleber F.M., Toniollo, Gilson H.
Format: Journal Article
Language:English
Published: Germany Blackwell Publishing Ltd 01-11-2018
Subjects:
Animals
Apoptosis
Apoptosis - physiology
Caspase
Caspase 3
Cats
cryopreservation
Cryopreservation - methods
Cryopreservation - veterinary
Cryoprotective Agents
Cryoprotectors
Epithelial Cells
felids
Female
Follicles
Fragmentation
Fragments
histology
Immunohistochemistry
Ovarian Follicle
Ovariectomy
Ovaries
ovary
Ovary - physiology
Perfusion
Queens
Vitrification
apoptosis
ovary
felids
immunohistochemistry
histology
cryopreservation
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Summary:Contents The aim of the present study was to investigate whether or not the size of the ovarian fragment influences its resistance to cryostorage. For that purpose, ovaries were collected from 34 queens (various breeds, age 1–5 year) by routine ovariectomy, transported to the laboratory and then sectioned in different sizes (3 mm × 3 mm × 3 mm, 5 mm × 3 mm × 3 mm and 7 mm × 3 mm × 3 mm) and randomly assigned to a control (GC3, GC5 and GC7, respectively) or vitrified (GV3, GV5 and GV7, respectively) groups. Vitrified‐warmed fragments were evaluated by histomorphology and immunohistochemistry (for apoptotic rates by using cleaved caspase‐3). Histological examination reveals that 72.97% of the follicles in GV3 and 72.58% in GV5 were normal while only 42.86% of the follicles in GV7. The main morphological alteration presented in all groups was a detachment of the epithelial cells. Similarly, immunohistochemistry evaluation using caspase 3 revealed a small proportion of apoptotic cells in GV3 (8.43%) while in GV7 30.43% of the cells expressed cleaved caspase‐3. These findings indicate that fragments sectioned in 3 mm × 3 mm × 3 mm (27 mm3) seem more adequate for perfusion of the cryoprotectant, causing less damage to the cell after vitrification‐warming.
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ISSN:0936-6768
1439-0531
DOI:10.1111/rda.13357