Search Results - "Gorn, Vladimir"

Quantitative Reverse Transcription–Polymerase Chain Reaction to Study mRNA Decay: Comparison of Endpoint and Real-Time Methods by Schmittgen, Thomas D., Zakrajsek, Brian A., Mills, Alan G., Gorn, Vladimir, Singer, Michael J., Reed, Michael W.

“…Four quantitative reverse transcription–PCR (RT-PCR) methods were compared to evaluate the time course of mRNA formation and decay. Mouse fibroblasts (NIH 3T3)…”
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3'-minor groove binder-DNA probes increase sequence specificity at PCR extension temperatures by Kutyavin, I V, Afonina, I A, Mills, A, Gorn, V V, Lukhtanov, E A, Belousov, E S, Singer, M J, Walburger, D K, Lokhov, S G, Gall, A A, Dempcy, R, Reed, M W, Meyer, R B, Hedgpeth, J

“…DNA probes with conjugated minor groove binder (MGB) groups form extremely stable duplexes with single-stranded DNA targets, allowing shorter probes to be used…”
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Novel DNA probes with low background and high hybridization-triggered fluorescence by Lukhtanov, Eugeny A, Lokhov, Sergey G, Gorn, Vladimir V, Podyminogin, Mikhail A, Mahoney, Walt

“…Novel fluorogenic DNA probes are described. The probes (called Pleiades) have a minor groove binder (MGB) and a fluorophore at the 5'-end and a non-fluorescent…”
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Reduced aggregation and improved specificity of G‐rich oligodeoxyribonucleotides containing pyrazolo[3,4‐d]pyrimidine guanine bases by Kutyavin, Igor V., Lokhov, Sergey G., Afonina, Irina A., Dempcy, Robert, Gall, Alexander A., Gorn, Vladimir V., Lukhtanov, Eugene, Metcalf, Mark, Mills, Alan, Reed, Michael W., Sanders, Sylvia, Shishkina, Irina, Vermeulen, Nicolaas M. J.

“…Guanine (G)‐rich oligodeoxyribonucleotides (ODNs) can form undesired complexes by self association through non‐Watson–Crick interactions. These aggregates can…”
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Oligonucleotides Containing 2-Aminoadenine and 2-Thiothymine Act as Selectively Binding Complementary Agents by Kutyavin, Igor V, Rhinehart, Rebecca L, Lukhtanov, Eugeny A, Gorn, Vladimir V, Meyer, Rich B, Gamper, Howard B

“…A pair of complementary oligodeoxynucleotides (ODNs) uniformly substituted with 2-aminoadenine (A‘) in place of adenine and 2-thiothymine (T‘) in place of…”
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Synthesis and Reactivity of Aryl Nitrogen Mustard−Oligodeoxyribonucleotide Conjugates by Reed, Michael W, Lukhtanov, Eugeny A, Gorn, Vladimir, Kutyavin, Igor, Gall, Alexander, Wald, Ansel, Meyer, Rich B

“…A versatile method is described for preparing aryl nitrogen mustard−oligodeoxyribonucleotide (mustard−ODN) conjugates under anhydrous conditions. The chemistry…”
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Sequence and Structure Dependence of the Hybridization-Triggered Reaction of Oligonucleotides Bearing Conjugated Cyclopropapyrroloindole by Lukhtanov, Eugeny A, Kutyavin, Igor V, Gorn, Vladimir V, Reed, Michael W, Adams, A. David, Lucas, Deborah D, Meyer, Rich B

“…Oligodeoxyribonucleotides (ODNs) with conjugated reactive groups are potential sequence-specific gene inactivating agents. The antitumor antibiotic CC-1065…”
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Structure-Activity Relationships of Cytotoxic Cholesterol-Modified DNA Duplexes by Reed, Michael W, Lukhtanov, Eugeny, Gorn, Vladimir, Lucas, Deborah D, Zhou, James H, Pai, S. Balakrishna, Cheng, Yung-chi, Meyer, Rich B

“…Short DNA duplexes with cholesterol linked at the 3'-terminus of each strand have unique, selective cytotoxic properties. The structural requirements for…”
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novel endonuclease IV post-PCR genotyping system by Kutyavin, Igor V, Milesi, Dave, Belousov, Yevgeniy, Podyminogin, Mikhail, Vorobiev, Alexei, Gorn, Vladimir, Lukhtanov, Eugeny A, Vermeulen, Nicolaas M.J, Mahoney, Walt

“…Here we describe a novel endonuclease IV (Endo IV) based assay utilizing a substrate that mimics the abasic lesions that normally occur in double-stranded DNA…”
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Antisense Oligonucleotides Containing Modified Bases Inhibit in Vitro Translation of Leishmania amazonensis mRNAs by Invading the Mini-exon Hairpin by Compagno, D, Lampe, J N, Bourget, C, Kutyavin, I V, Yurchenko, L, Lukhtanov, E A, Gorn, V V, Gamper, Jr, H B, Toulmé, J J

“…Complementary oligodeoxynucleotides (ODNs) that contain 2-aminoadenine and 2-thiothymine interact weakly with each other but form stable hybrids with…”
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3'-Minor groove binder-DNA probes increase sequence specificity at PCR extension temperatures by Kutyavin, I. V.

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Synthesis and Evaluation of a Triplex-Forming Oligonucleotide−Pyrrolobenzodiazepine Conjugate by Zhilina, Zhanna V, Ziemba, Amy J, Trent, John O, Reed, Michael W, Gorn, Vladimir, Zhou, Qun, Duan, Wenhu, Hurley, Laurence, Ebbinghaus, Scot W

“…In most cases, unmodified oligonucleotides designed as antigene molecules are incapable of binding to DNA with sufficient stability to prevent gene expression…”
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Repair of triplex-directed DNA alkylation by nucleotide excision repair by Ziemba, A, Derosier, L C, Methvin, R, Song, C Y, Clary, E, Kahn, W, Milesi, D, Gorn, V, Reed, M, Ebbinghaus, S

“…Triplex-forming oligonucleotides (TFOs) are being investigated as highly specific DNA binding agents to inhibit the expression of clinically relevant genes. So…”
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Reduced aggregation and improved specificity of G-rich oligodeoxyribonucleotides containing pyrazolo3,4-dpyrimidine guanine bases by Kutyavin, Igor V, Lokhov, Sergey G, Afonina, Irina A, Dempcy, Robert, Gall, Alexander A, Gorn, Vladimir V, Lukhtanov, Eugene, Metcalf, Mark, Mills, Alan, Reed, Michael W, Sanders, Sylvia, Shishkina, Irina, Vermeulen, Nicolaas M J

“…Guanine (G)-rich oligodeoxyribonucleotides (ODNs) can form undesired complexes by self association through non-Watson-Crick interactions. These aggregates can…”
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Minor Groove DNA Alkylation Directed by Major Groove Triplex Forming Oligodeoxyribonucleotides by Lukhtanov, Eugeny A., Mills, Alan G., Kutyavin, Igor V., Gorn, Vladimir V., Reed, Michael W., Meyer, Rich B.

“…We describe sequence-specific alkylation in the minor groove of double-stranded DNA by a hybridization-triggered reactive group conjugated to a triplex forming…”
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Synthesis of oligonucleotide conjugates in anhydrous dimethyl sulfoxide by Milesi, D, Kutyavin, I, Lukhtanov, E A, Gorn, V V, Reed, M W

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Solution structure of a highly stable DNA duplex conjugated to a minor groove binder by Kumar, Surat, Foti, Matthew, West, John, Schweitzer, Barry I., Reed, Michael W., Gamper, Howard B., Gorn, Vladimir V., Lukhtanov, Eugeny A., Meyer, Rich B.

“…The tripeptide 1,2-dihydro-(3H)-pyrrolo[3,2-e]indole-7-carboxylate (CDPI3) binds to the minor groove of DNA with high affinity. When this minor groove binder…”
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The affinity of the Klenow fragment of E. coli DNA-polymerase 1 to primers containing bases noncomplementary to the template and hairpin-like elements by Ljach, Marija V., Kolocheva, Tatjana I., Gorn, Vladimir V., Levina, Asja S., Nevinsky, Georgy A.

“…The K m and V max values for a set of primers: d(pT) n (pC)(pT) m ( n=3–9. m=0–7) and d(pT) 4 (pCpG) 4 (pT) 4 ( k=1–5) have been estimated. Poly(dA) was used…”
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