Determination of adenosine deaminase activity and its isoenzymes for diagnosis of pleural effusions

We aimed to investigate adenosine deaminase (ADA) activity and the activities of its ADA1 and ADA2 isoenzymes in pleural effusions and also sera with different aetiological origins. The pleural effusions of 87 patients were examined. The patients were separated into four groups: transudates, parapne...

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Bibliographic Details
Published in:	Respirology (Carlton, Vic.) Vol. 5; no. 4; pp. 321 - 324
Main Authors:	Gorguner, M, Cerci, M, Gorguner, I
Format:	Journal Article
Language:	English
Published:	Australia 01-12-2000
Subjects:	Adenosine Deaminase - analysis Adenosine Deaminase - blood Adult Biomarkers - blood Colorimetry Exudates and Transudates Female Heart Failure - complications Humans Isoenzymes - analysis Isoenzymes - blood Liver Cirrhosis - complications Male Middle Aged Neoplasms - complications Pleural Effusion - chemistry Pleural Effusion - diagnosis Pleural Effusion - enzymology Pleural Effusion - etiology Pneumonia - complications Renal Insufficiency - complications Sensitivity and Specificity Tuberculosis, Pleural - complications
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Summary:	We aimed to investigate adenosine deaminase (ADA) activity and the activities of its ADA1 and ADA2 isoenzymes in pleural effusions and also sera with different aetiological origins. The pleural effusions of 87 patients were examined. The patients were separated into four groups: transudates, parapneumonic, malignant, and tuberculous effusions. The cases were also designated as tuberculous or non-tuberculous group. Adenosine deaminase activity was determined by the colorimetric method described by Giusti and Galanti. The intermean differences were statistically significant for total ADA, ADA1 and ADA2, except for pleural fluid ADA1 in the malignant group when compared to the tuberculous effusion group. The intermean differences between the tuberculous and non-tuberculous group were statistically significant for all three parameters except for pleural fluid and serum ADA1 activity. The sensitivities of total ADA, ADA1 and ADA2 activities for tuberculosis were 91, 57 and 93%, respectively; their specificities 89, 88 and 92%, respectively; their positive predictive values 82, 70 and 86%; and their diagnostic accuracies 89, 76 and 92%, respectively, in pleural fluid. Determination of ADA and its isoenzymes can help to differentiate the causes of pleural effusion. Increased ADA2 activity is a striking marker of tuberculous effusions. In contrast, increased ADA1 activity was significantly elevated in parapneumonic effusions.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1323-7799 1440-1843
DOI:	10.1046/j.1440-1843.2000.00270.x