Chromatographic profile, in silico and in vivo study of the pharmacokinetic and toxicological properties of major constituent present in kefir, the kefiran

Kefiran is a polysaccharide present in kefir grains that have been widely explored due to its potential health benefits. The objective of this work was to characterize and quantify the components present in the ethanolic extract of milk kefir grains; to study its pharmacokinetic and toxicological pr...

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Bibliographic Details
Published in:	Toxicology research (Cambridge) Vol. 11; no. 3; pp. 520 - 528
Main Authors:	Barros, Susy Érika de Lima, de Lima, Henrique Barros, Gonçalves, Leandra Karoline Alves, Correia, Lenir Cabral, Brito, Maiara de Fátima de Brito, Barcelos, Mariana Pegrucci, Silva, Guilherme Martins, da Silva, Carlos Henrique Tomich de Paula, da Costa, Rafael Garrett, Cruz, Rodrigo Alves Soares, Carvalho, José Carlos Tavares, da Silva Hage-Melim, Lorane Izabel
Format:	Journal Article
Language:	English
Published:	England Oxford University Press 01-06-2022
Subjects:	functional foods kefiran in silico polysaccharide ADME/Tox kefir
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Summary:	Kefiran is a polysaccharide present in kefir grains that have been widely explored due to its potential health benefits. The objective of this work was to characterize and quantify the components present in the ethanolic extract of milk kefir grains; to study its pharmacokinetic and toxicological properties in silico and evaluate the acute toxicity of the kefiran in zebrafish. The prediction of pharmacokinetic properties was performed by QikProp software. In silico toxicity assessment was performed using the DEREK (deductive estimate of risk from existing knowledge) software. In the chromatographic, kefiran was identified as the major component. Results showed that the kefiran had low human oral absorption and intestinal absorption its due poor solubility profile; low logP value, indicating its lipophilicity and the low MDCK and Caco-2 cells permability, and unable to cross the blood-brain barrier. Kefiran did not present any structural warning for in silico toxicity. In zebrafish, the dose of 2,000 mg/kg of kefiran produced nonsignificant alterations in the analyzed organs. It can be said then that kefiran has an acceptable degree of safety for use in the development of drugs or functional foods. Further research such as in vivo testing to confirm its pharmacological potential is currently underway.
ISSN:	2045-452X 2045-4538 2045-4538
DOI:	10.1093/toxres/tfac032