Search Results - "Gommers, F J"

The C-terminal KDEL sequence increases the expression level of a single-chain antibody designed to be targeted to both the cytosol and the secretory pathway in transgenic tobacco by Schouten, A, Roosien, J, van Engelen, F A, de Jong, G A, Borst-Vrenssen, A W, Zilverentant, J F, Bosch, D, Stiekema, W J, Gommers, F J, Schots, A, Bakker, J

“…The effects of subcellular localization on single-chain antibody (scFv) expression levels in transgenic tobacco was evaluated using an scFv construct of a…”
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Gene pool similarities of potato cyst nematode populations assessed by AFLP analysis by Folkertsma, R.T. (Wageningen Agricultural University, Wageningen, The Netherlands.), Rouppe van der Voort, J.N.A.M, Groot, K.E. de, Zandvoort, P.M. van, Schots, A, Gommers, F.J, Helder, J, Bakker, J

“…AFLP was used to characterize 24 potato cyst nematode populations. This novel DNA fingerprinting technique enabled the identification of 987 marker loci by…”
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Changing concepts and molecular approaches in the management of virulence genes in potato cyst nematodes by Bakker, J, Folkertsma, R.T, Rouppe van der Voort, J.N.A.M, Boer, J.M. de, Goomers, F.J

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In planta monitoring of the activity of two constitutive promoters, CaMV 35S and TR2', in developing feeding cells induced by Globodera rostochiensis using green fluorescent protein in combination with confocal laser scanning microscopy by GOVERSE, A, BIESHEUVEL, J, WIJERS, G.-J, GOMMERS, F. J, BAKKER, J, SCHOTS, A, HELDER, J

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Endogenous Cellulases in Animals: Isolation of β -1,4-Endoglucanase Genes from two Species of Plant-Parasitic Cyst Nematodes by Smant, Geert, Jack P. W. G. Stokkermans, Yan, Yitang, De Boer, Jan M., Baum, Thomas J., Wang, Xiaohong, Hussey, Richard S., Gommers, Fred J., Henrissat, Bernard, Davis, Eric L., Helder, Johannes, Schots, Arjen, Bakker, Jaap

“…β -1,4-Endoglucanases (EGases, EC 3.2.1.4) degrade polysaccharides possessing β -1,4-glucan backbones such as cellulose and xyloglucan and have been found…”
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Secretory granule proteins from the subventral esophageal glands of the potato cyst nematode identified by monoclonal antibodies to a protein fraction from second-stage juveniles by Boer, J.M. de (Wageningen Agricultural University, Wageningen, The Netherlands.), Smant, G, Goverse, A, Davis, E.L, Overmars, H.A, Pomp, H.R, Gent-Pelzer, M. van, Zilverentant, J.F, Stokkermans, J.P.W.G, Hussey, R.S

“…Sodium dodecyl sulfate-extracted proteins from second stage juveniles (J2) of the potato cyst nematode Globodera rostochiensis were fractionated by preparative…”
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Single juveniles of the potato cyst nematodes Globodera rostochiensis and G. pallida differentiated by randomly amplified polymorphic DNA by Roosien, J, Van Zandvoort, P M, Folkertsma, R T, Van der Voort, J N, Goverse, A, Gommers, F J, Bakker, J

“…Random amplified polymorphic DNA (RAPD) offers a potential basis for the development of a diagnostic assay to differentiate the potato cyst nematode species…”
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Photoactivation of the nematicidal compound alpha-terthienyl from roots of marigolds (Tagetes species). A possible singlet oxygen role by Bakker, J, Gommers, F J, Nieuwenhuis, I, Wynberg, H

“…The nematicidal compound alpha-terthienyl from roots of Tagetes species generates upon irradiation with near ultraviolet light reactive oxygen species on which…”
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Use of allele specificity of comigrating AFLP markers to align genetic maps from different potato genotypes by Rouppe van der Voort, J N, van Zandvoort, P, van Eck, H J, Folkertsma, R T, Hutten, R C, Draaistra, J, Gommers, F J, Jacobsen, E, Helder, J, Bakker, J

“…The allele specificity of AFLP markers was assessed in five relatively unrelated potato genotypes. To this end, two diploid mapping populations of potato, F1SH…”
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Cluster analysis of 36 Globodera pallida field populations using two sets of molecular markers by Folkertsma, R T, De Groot, KE, Van Koert, PHG, Van Gent-Pelzer, MPE, Van der Voort, JNAMR, Schots, A, Bakker, J, Gommers, F J, Helder, J

“…Thirty-six populations of the potato cyst nematode Globodera pallida, all collected in the Netherlands, were analysed twice: by two-dimensional gel…”
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Improving scFv antibody expression levels in the plant cytosol by Schouten, Alexander, Roosien, Jan, de Boer, Jan M, Wilmink, Annemiek, Rosso, Marie-Noëlle, Bosch, Dirk, Stiekema, Willem J, Gommers, Fred J, Bakker, Jaap, Schots, Arjen

“…Expression of single-chain antibody fragments (scFvs) in the plant cytosol is often cumbersome. It was unexpectedly shown that addition at the C-terminus of…”
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Coordinate expression of antibody subunit genes yields high levels of functional antibodies in roots of transgenic tobacco by Engelen, F.A. van (Centrum voor Plantenveredelings- en Reproduktieonderzoek DLO, Wageningen (Netherlands)), Schouten, A, Molthoff, J.W, Roosien, J, Salinas, J, Dirkse, W.G, Schots, A, Bakker, J, Gommers, F.J, Jongsma, M.A, Bosch, D, Stiekema, W.J

“…To explore the feasibility of employing antibodies to obtain disease resistance against plant root pathogens, we have studied the expression of genes encoding…”
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Circumventing the diapause of potato cyst nematodes by Janssen, R., Bakker, J., Gommers, F. J.

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Nuclear and mitochondrial DNA polymorphisms in three mitotic parthenogenetic Meloidogyne spp by DAUTOVA, Makedonka, OVERMARS, Hein, BAKKER, Jaap, SMANT, Geert, GOMMERS, Fred J

“…In order to expand our understanding of the genetics of root-knot nematodes, variation in nuclear DNA and mitochondrial DNA in Meloidogyne incognita, M…”
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Expression and Functional Characterization of a Single Chain FV Antibody Directed against Secretions Involved in Plant Nematode Infection Process by Rosso, Marie-Noëlle, Schouten, Alexander, Roosien, Jan, Borst-Vrenssen, Tanja, Hussey, Richard S., Gommers, Fred J., Bakker, Japp, Schots, Arjen, Abad, Pierre

“…Expression in plants of antibodies directed against proteins essential for pathogenesis could provide an alternative approach to engineer new resistance traits…”
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Inter- and intraspecific variation between populations of Globodera rostochiensis and G. pallida revealed by random amplified polymorphic DNA by Folkertsma, R.T. (Wageningen Agricultural University, Wageningen, Netherlands), Rouppe van der Voort, J.N.A.M, Gent-Pelzer, M.P.E. van, Groot, K.E. de, Bos, W.J. van den, Schots, A, Bakker, J, Gommers, F.J

“…The genetic relationships between populations of the potato cyst nematode species Globodera rostochiensis and G. pallida were analyzed using random amplified…”
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Water transport through tomato roots infected with Meloidogyne incognita by Dorhout, R. (University of Utrecht, Utrecht, The Netherlands), Gommers, F.J, Kolloffel, C

“…The effect of Meloidogyne incognita on water flow in tomato roots was investigated in rooted split-stem cuttings. Total water flow through infected root parts…”
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Investigation of P and S distributions in the roots of Tagetes patula L. using micro-PIXE by Makjanic, J, Vis, R.D, Groneman, A.F, Gommers, F.J, Henstra, S

“…A proton microprobe for energy-dispersive PIXE-analysis has been used to measure elemental distributions of sulphur and other elements in roots of Tagetes…”
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Transport of an apoplastic fluorescent dye to feeding sites induced in tomato roots by Meloidogyne incognita by Dorhout, R, Kolloffel, C, Gommers, F.J

“…The fluorescent dye Tinopal CBS was used to investigate whether there is an apoplastic pathway between the stele and the cortes in tomato roots infected with…”
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Species-specific and thermostable proteins from second-stage larvae of Globodera rostochiensis and G. pallida by Bakker, J, Schots, A, Bouwman-Smits, L, Gommers, F.J

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