Search Results - "Gomi, Keiko"

Improvement in the thermal stability of Mucor prainii-derived FAD-dependent glucose dehydrogenase via protein chimerization by Masakari, Yosuke, Hara, Chiaki, Araki, Yasuko, Gomi, Keiko, Ito, Kotaro

“…•Mr144–297 was more heat resistant and stable than glucose oxidase from A. niger.•Mr144–297 had both high affinity and accurate substrate specificity for…”
Get full text

Soy sauce-like seasoning enhances the growth of Agathobacter rectalis and the production of butyrate, propionate, and lactate by HAYASHI, Kanako, UCHIDA, Riichiro, HORIBA, Taro, KAWAGUCHI, Tomohiro, GOMI, Keiko, GOTO, Yoshiyuki

“…The short-chain fatty acids responsible for gut homeostasis are volatile fatty acids produced by commensal bacteria in the gut as fermentation products from…”
Get full text

High-level heterologous protein production using an attenuated selection marker in Aspergillus sojae by Araki, Yasuko, Yuzuki, Masanobu, Masakari, Yosuke, Sato, Atsushi, Gomi, Keiko, Hara, Seiichi

“…Filamentous fungi, including Aspergillus sojae, are essential for the industrial production of enzymes. Although multi-copy introduction of a gene encoding the…”
Get full text

Heterologous production of ascofuranone and ilicicolin A in Aspergillus sojae by Araki, Yasuko, Shinohara, Yasutomo, Hara, Seiichi, Sato, Atsushi, Sakaue, Ryoichi, Gomi, Keiko, Kita, Kiyoshi, Ito, Kotaro

“…Ascofuranone and its precursor, ilicicolin A, are secondary metabolites with various pharmacological activities that are produced by Acremonium egyptiacum. In…”
Get full text

Synthesis and inhibitory activity of substrate-analog fructosyl peptide oxidase inhibitors by Watanabe, Bunta, Ichiyanagi, Atsushi, Hirokawa, Kozo, Gomi, Keiko, Nakatsu, Toru, Kato, Hiroaki, Kajiyama, Naoki

“…[Display omitted] Fructosyl peptide oxidases (FPOXs) play a crucial role in the diagnosis of diabetes. Their main function is to cleave fructosyl amino acids…”
Get full text

Improving the Stability of Protein–Protein Interaction Assay FlimPIA Using a Thermostabilized Firefly Luciferase by Ohmuro-Matsuyama, Yuki, Gomi, Keiko, Shimoda, Takuya, Yamaji, Hideki, Ueda, Hiroshi

“…The protein–protein interaction assay is a key technology in various fields, being applicable in drug screening as well as in diagnosis and inspection, wherein…”
Get full text

Discrimination of methicillin-resistant Staphylococcus aureus from methicillin-susceptible Staphylococcus aureus or coagulase-negative staphylococci by detection of penicillin-binding protein 2 and penicillin-binding protein 2′ using a bioluminescent enzyme immunoassay by Shiga, Kazuki, Gomi, Keiko, Nishimura, Motoi, Watanabe, Masaharu, Nomura, Fumio, Kajiyama, Naoki

“…For the discrimination of methicillin-resistant S. aureus (MRSA) from methicillin-susceptible S. aureus (MSSA) or coagulase-negative staphylococci (CNS), we…”
Get full text

Oxyluciferin, a Luminescence Product of Firefly Luciferase, Is Enzymatically Regenerated into Luciferin by Gomi, Keiko, Kajiyama, Naoki

“…The activity regenerating luciferin from the luminescent product oxyluciferin was found in the protein fraction of a lantern extract from Photinus pyralis. The…”
Get full text

Molecular cloning and expression of the cDNAs encoding luciferin-regenerating enzyme from Luciola cruciata and Luciola lateralis by Gomi, Keiko, Hirokawa, Kozo, Kajiyama, Naoki

“…In the firefly light organ, oxyluciferin, a product of the light-emitting reaction of firefly luciferase, is thought to be converted into luciferin…”
Get full text

Crystallization and preliminary crystallographic analysis of two eukaryotic fructosyl peptide oxidases by Ichiyanagi, Atsushi, Hirokawa, Kozo, Gomi, Keiko, Nakatsu, Toru, Kato, Hiroaki, Kajiyama, Naoki

“…Fructosyl peptide oxidase (FPOX) catalyses the oxidation of α‐glycated dipeptides such as Nα‐(1‐deoxy‐D‐fructos‐1‐yl)‐L‐valyl‐L‐histidine (Fru‐ValHis) and is…”
Get full text

Distribution and properties of novel deglycating enzymes for fructosyl peptide in fungi by HIROKAWA, Kozo, GOMI, Keiko, BAKKE, Mikio, KAJIYAMA, Naoki

“…Our fungal culture collection was screened for fructosyl peptide oxidase, an enzyme that could be used for the determination of glycated hemoglobin in diabetic…”
Get full text

Heterologous production of ascofuranone and ilicicolin A in Aspergillus sojae by Araki, Yasuko, Shinohara, Yasutomo, Hara, Seiichi, Sato, Atsushi, Sakaue, Ryoichi, Gomi, Keiko, Kita, Kiyoshi, Ito, Kotaro

“…Ascofuranone and its precursor, ilicicolin A, are secondary metabolites with various pharmacological activities that are produced by Acremonium egyptiacum. In…”
Get full text

Evaluation of protein-ligand interactions using the luminescent interaction assay FlimPIA with streptavidin-biotin linkage by Ohmuro-Matsuyama, Yuki, Yamashita, Takahiro, Gomi, Keiko, Yamaji, Hideki, Ueda, Hiroshi

“…Post-translational modifications, such as phosphorylation, are crucial in the regulation of protein-protein interactions and protein function in cell…”
Get full text

Soy sauce-like seasoning enhances the growth of Agathobacter rectalis and the production of butyrate, propionate, and lactate by HAYASHI, Kanako, UCHIDA, Riichiro, HORIBA, Taro, KAWAGUCHI, Tomohiro, GOMI, Keiko, GOTO, Yoshiyuki

“…The short-chain fatty acids responsible for gut homeostasis are volatile fatty acids produced by commensal bacteria in the gut as fermentation products from…”
Get full text

High-level heterologous protein production using an attenuated selection marker in Aspergillus sojae by Araki, Yasuko, Yuzuki, Masanobu, Masakari, Yosuke, Sato, Atsushi, Gomi, Keiko, Hara, Seiichi

“…Filamentous fungi, including Aspergillus sojae, are essential for the industrial production of enzymes. Although multi-copy introduction of a gene encoding the…”
Get full text

Novel glucose dehydrogenase from Mucor prainii: Purification, characterization, molecular cloning and gene expression in Aspergillus sojae by Satake, Ryoko, Ichiyanagi, Atsushi, Ichikawa, Keiichi, Hirokawa, Kozo, Araki, Yasuko, Yoshimura, Taro, Gomi, Keiko

“…Glucose dehydrogenase (GDH) is of interest for its potential applications in the field of glucose sensors. To improve the performance of glucose sensors, GDH…”
Get full text

Cloning and expression of a highly active recombinant alkaline phosphatase from psychrotrophic Cobetia marina by Nasu, Eriko, Ichiyanagi, Atsushi, Gomi, Keiko

“…Alkaline phosphatase catalyzes the hydrolysis of phosphomonoesters and is widely used in molecular biology techniques and clinical diagnostics. We expressed a…”
Get full text

An isomorphous replacement method for efficient de novo phasing for serial femtosecond crystallography by Yamashita, Keitaro, Pan, Dongqing, Okuda, Tomohiko, Sugahara, Michihiro, Kodan, Atsushi, Yamaguchi, Tomohiro, Murai, Tomohiro, Gomi, Keiko, Kajiyama, Naoki, Mizohata, Eiichi, Suzuki, Mamoru, Nango, Eriko, Tono, Kensuke, Joti, Yasumasa, Kameshima, Takashi, Park, Jaehyun, Song, Changyong, Hatsui, Takaki, Yabashi, Makina, Iwata, So, Kato, Hiroaki, Ago, Hideo, Yamamoto, Masaki, Nakatsu, Toru

“…Serial femtosecond crystallography (SFX) with X-ray free electron lasers (XFELs) holds great potential for structure determination of challenging proteins that…”
Get full text

Highly sensitive and rapid tandem bioluminescent immunoassay using aequorin labeled Fab fragment and biotinylated firefly luciferase by Ito, Katsutoshi, Nishimura, Waka, Maeda, Masako, Gomi, Keiko, Inouye, Satoshi, Arakawa, Hidetoshi

“…We established a simultaneous bioluminescent assay utilizing aequorin (Aq) and biotinylated firefly luciferase (b-Luc); furthermore, we developed a highly…”
Get full text

Senescence marker protein-30 is a unique enzyme that hydrolyzes diisopropyl phosphorofluoridate in the liver by Kondo, Yoshitaka, Ishigami, Akihito, Kubo, Sachiho, Handa, Setsuko, Gomi, Keiko, Hirokawa, Kozo, Kajiyama, Naoki, Chiba, Tsuyoshi, Shimokado, Kentaro, Maruyama, Naoki

“…Senescence marker protein-30 (SMP30) was originally identified as a novel protein in the rat liver, the expression of which decreases androgen-independently…”
Get full text